Original Article. Study of the cytotoxic/toxic potential of the novel anticancer selenodiazoloquinolone on fibroblast cells and 3D skin model

Jantová, Soňa; Topoľská, Dominika; Janošková, Michaela; Panik, Miroslav; Milata, Viktor

doi:10.1515/intox-2016-0014

Cited by 8 publications

(4 citation statements)

References 34 publications

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“…10 ). The monocyte/macrophage-like cell line RAW 264.7, originating from Abelson leukemia virus transformed cell line derived from BALB/c mice, has been one of the most widely used cell line for evaluating anti-inflammatory property [ 71 ] . The cell lines were obtained from healthy mouse model.…”

Section: Resultsmentioning

confidence: 99%

Valorization of coffee bean processing waste for the sustainable extraction of biologically active pectin

Divyashri,

Krishna Murthy,

Ragavan

et al. 2023

Heliyon

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Section: Resultsmentioning

confidence: 99%

Valorization of coffee bean processing waste for the sustainable extraction of biologically active pectin

Divyashri,

Krishna Murthy,

Ragavan

et al. 2023

Heliyon

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“…It is worth mentioning that the AF-Ac at 50 µg/mL was not cytotoxic for PBMC and fibroblast cells. The objective of using PBMC and fibroblast cells as controls is to demonstrate that the AF-Ac fraction is not cytotoxic to healthy cells, since these human non-cancer cells are potentially useful models for cell viability testing using plant extracts [47][48][49][50]. Also, PBMC cells represent the whole metabolic status and an excellent model for assessing the differences or changes associated with pathophysiological conditions [47,51].…”

Section: Discussionmentioning

confidence: 99%

Alkaloid and acetogenin-rich fraction fromAnnona crassiflorafruit peel inhibits proliferation and migration of human liver cancer HepG2 cells

Justino

Florentino

França

et al. 2021

Preprint

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Plant species from Annonaceae are commonly used in traditional medicine to treat various cancer types. This study aimed to investigate the antiproliferative potential of an alkaloid and acetogenin-rich fraction from the fruit peel of Annona crassiflora in HepG2 cells. A liquid-liquid fractionation was carried out on the ethanol extract of A. crassiflora fruit peel in order to obtain an alkaloid and acetogenin-rich fraction (AF-Ac). Cytotoxicity, proliferation and migration were evaluated in the HepG2 cells, as well as the proliferating cell nuclear antigen (PCNA), vinculin and epidermal growth factor receptor (EGFR) expression. In addition, intracellular Ca2+ was determined using Fluo4-AM and fluorescence microscopy. First, 9 aporphine alkaloids and 4 acetogenins that had not yet been identified in the fruit peel of A. crassiflora were found in AF-Ac. The treatment with 50 µg/mL AF-Ac reduced HepG2 cell viability, proliferation and migration (p < 0.001), which is in accordance with the reduced expression of PCNA and EGFR levels (p < 0.05). Furthermore, AF-Ac increased intracellular Ca2+ in the HepG2 cells, mobilizing intracellular calcium stores, which might be involved in the anti-migration and anti-proliferation capacities of AF-Ac. Our results support the growth-inhibitory potential of AF-Ac on HepG2 cells and suggest that this effect is triggered, at least in part, by PCNA and EGFR modulation and mobilization of intracellular Ca2+. This study showed biological activities not yet described for A. crassiflora fruit peel, which provide new possibilities for further in vivo studies to assess the antitumoral potential of A. crassiflora, especially its fruit peel.

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“…It is worth mentioning that the AF-Ac at 50 μg/mL was not cytotoxic for PBMC and fibroblast cells. The objective of using PBMC and fibroblast cells as controls is to demonstrate that the AF-Ac fraction is not cytotoxic to healthy cells, since these human non-cancer cells are potentially useful models for cell viability testing using plant extracts [47][48][49][50]. Also, PBMC cells represent the whole metabolic status and an excellent model for assessing the differences or changes associated with pathophysiological conditions [47,51].…”

Section: Plos Onementioning

confidence: 99%

Alkaloid and acetogenin-rich fraction from Annona crassiflora fruit peel inhibits proliferation and migration of human liver cancer HepG2 cells

et al. 2021

View full text Add to dashboard Cite

Plant species from Annonaceae are commonly used in traditional medicine to treat various cancer types. This study aimed to investigate the antiproliferative potential of an alkaloid and acetogenin-rich fraction from the fruit peel of Annona crassiflora in HepG2 cells. A liquid-liquid fractionation was carried out on the ethanol extract of A. crassiflora fruit peel in order to obtain an alkaloid and acetogenin-rich fraction (AF-Ac). Cytotoxicity, proliferation and migration were evaluated in the HepG2 cells, as well as the proliferating cell nuclear antigen (PCNA), vinculin and epidermal growth factor receptor (EGFR) expression. In addition, intracellular Ca2+ was determined using Fluo4-AM and fluorescence microscopy. First, 9 aporphine alkaloids and 4 acetogenins that had not yet been identified in the fruit peel of A. crassiflora were found in AF-Ac. The treatment with 50 μg/mL AF-Ac reduced HepG2 cell viability, proliferation and migration (p < 0.001), which is in accordance with the reduced expression of PCNA and EGFR levels (p < 0.05). Furthermore, AF-Ac increased intracellular Ca2+ in the HepG2 cells, mobilizing intracellular calcium stores, which might be involved in the anti-migration and anti-proliferation capacities of AF-Ac. Our results support the growth-inhibitory potential of AF-Ac on HepG2 cells and suggest that this effect is triggered, at least in part, by PCNA and EGFR modulation and mobilization of intracellular Ca2+. This study showed biological activities not yet described for A. crassiflora fruit peel, which provide new possibilities for further in vivo studies to assess the antitumoral potential of A. crassiflora, especially its fruit peel.

show abstract

Original Article. Study of the cytotoxic/toxic potential of the novel anticancer selenodiazoloquinolone on fibroblast cells and 3D skin model

Cited by 8 publications

References 34 publications

Valorization of coffee bean processing waste for the sustainable extraction of biologically active pectin

Valorization of coffee bean processing waste for the sustainable extraction of biologically active pectin

Alkaloid and acetogenin-rich fraction fromAnnona crassiflorafruit peel inhibits proliferation and migration of human liver cancer HepG2 cells

Alkaloid and acetogenin-rich fraction from Annona crassiflora fruit peel inhibits proliferation and migration of human liver cancer HepG2 cells

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