Original article Thin-layer chromatography and in vitro free radical scavenging activity of few medicinal plants from the surroundings of Junagadh, Gujarat, India

Abstract

A collection of medicinal plants was done from the ambience of Junagadh district, Gujarat, India. These plants were subsequently screened to know their phytochemical potential. The in vitro antioxidant activity was also evaluated using DPPH and nitric oxide scavenging methods. Qualitative phytochemical screening and total phenolic content have been evaluated for each plant extract. TLC showed the presence of various phytochemicals like alkaloids, flavonoids, phenolics, glycosides, saponins, sterols and tannins. Methanol and water extracts of plants exhibited the highest amount of phenolic content. There was a difference in per cent inhibition of DPPH and nitric oxide scavenging activity showed by extracts. In the case of DPPH scavenging activity, water extracts of Peltophorum pterocarpum (DC.) K. Heyne leaves and Syzygium cumini (L.) Skeels leaves showed the highest free radical inhibition up to 76.37 ± 0.42% and 67.49 ± 0.51%, respectively. In the case of nitric oxide scavenging action, all the extracts of Bauhinia variegata L. bark and P. pterocarpum leaves showed nitric oxide inhibition more than 75%. The plants like B. variegata, P. pterocarpum and S. cumini revealed high level of phenolic compounds. In conclusion, extracts of B. variegata, P. pterocarpum and S. cumini leaves can be served as the vital source of phenoilcs and flavonoids for having antioxidant potential.

Antidiabetic, antioxidant and anti-inflammatory activity of medicinal plants collected from nearby area of Junagadh, Gujarat

“…It is postulated that antioxidant activity is mainly due to the phenolic content present in the plant. As an important phytochemical bearing very reactive hydroxyl group, phenolics have attributed antioxidant action (Bidchol et al, 2011;Bhatt et al, 2019a). In this study, in vitro antiinflammatory activity has not been seen with fruits of L. echinata.…”

“…Saurashtra region of Gujarat is a large biodiversity region with several medicinal plants. Since a long time, these medicinal plants are used in the ethnoveterinary practices as well as in the ailments of human or animals (Bhatt et al, 2019a). The main advantages of medicinal plants are, they are economical, readily available and has no side effects.…”

Status of Indian Medicinal Plants in Terms of Antioxidant Activity (In Vitro)

“…Coriandrum sativum L. and Spilanthes acmella L. was investigated by Bala et al, (2019), Kumari et al, (2019), Jeya et al, 2019 andThakur et al, (2019), using DPPH free radical scavenging assay. Bhatt et al, (2019) selected some plants from Junagadh, Gujarat for their antioxidant activity analysis and concluded that among all plants and different solvent extracts, aqueous extract of Peltophorum pterocarpum (DC.) K. Heyne and Syzygium cumini (L.) Skeels leaves presented highest DPPH radical scavenging ability while all the extracts of Bauhinia variegate L. Nag et al, (2019), using DNA protection assay for their antioxidant potential.…”