ORIGINAL RESEARCH: Evaluation &amp; control of potential sensitizing &amp; irritating chemical components in natural rubber latex extracted from the industrial crop guayule

Cornish, Katrina; Williams, Jali; Kirk, Melissa; Teetor, Valerie H.; Ray, Dennis T.

doi:10.1089/ind.2009.5.245

Cited by 15 publications

(8 citation statements)

References 19 publications

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“…For humans, the sensitizing power of guayulin A is much smaller than for laboratory animals. Hence, the possibility that traces of guayulin A may be present in the guayule rubber at a sensitizing level seems to be remote at the moment [16]. In analogy to what was observed for sesquiterpene lactones in most of the species of Parthenum genus, it is likely that guayulins may also exhibit other significant biological properties (i.e., antibacterial and anticancer activities) [15].…”

Section: Introductionmentioning

confidence: 88%

“…Until now, the analytical methods reported in literature for the measurement of the amount of guayulins in Guayule resin [3,4,8,11,13,[17][18][19] or latex [16] are scarce and all lacking of any validation protocol. Obviously, chromatographic methods are dominant in the analytical characterization of such analytes and, among them, HPLC is the preferred approach.…”

Section: Introductionmentioning

confidence: 99%

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Assessment, Validation and Application to Real Samples of an RP-HPLC Method for the Determination of Guayulins A, B, C and D in Guayule Shrub

et al. 2018

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Guayule (Parthenium argentatum Gray) is a shrub native to the arid regions of Mexico. In the last decades, significant attention to its cultivation has arisen because it is the raw material for the production of hypoallergenic natural rubber. Guayule biomass also contains high amounts of resin, which is not normally exploited in any way. Among other sesquiterpenic esters, guayulins (i.e., the parteniol esters of cinnamic acid, guayulin A, or of anisic acid, guayulin B) are contained in resin. In addition, minor amounts of guayulin C and guayulin D are formed by degradation/oxidation of guayulins A and B, respectively. Guayulins likely act as cinnamate and p-anisate reservoirs for the Guayule shrub; in addition, it has been postulated that they might have a key role in the chemical defense system of Guayule. Furthermore, it seems reasonable that guayulins may possess significant biological properties (e.g., antibacterial and anticancer activities), in close analogy with those shown by sesquiterpene lactones contained in many other species of Parthenum genus. As a matter of fact, guayulins A and B play an important role in the synthesis of antineoplastics used in breast cancer treatment. In this contribution we propose an original and validated RP-HPLC approach to the simultaneous quantification of guayulins A, B, C and D. The procedure of resin extraction from Guayule biomass has been optimized in terms of both extraction method and solvent. RP-HPLC separation has been accomplished by an Ascentis ® C18 column under isocratic elution with a 80:20 (v:v) acetonitrile:water mixture. Validation was carried out in terms of limits of detection and quantification, linearity, precision, and trueness. Finally, the method was tested with a number of fresh and seasoned samples of spontaneous Guayule shrub from Mexico.

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Section: Introductionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Assessment, Validation and Application to Real Samples of an RP-HPLC Method for the Determination of Guayulins A, B, C and D in Guayule Shrub

et al. 2018

View full text Add to dashboard Cite

show abstract

“…It is evident that the continuous rise of interest in the properties of guayulins needs the development of reliable, accurate and sensitive analytical methods for their identification and quantification. Until now, the analytical methods reported in literature for the measurement of the amount of guayulins in Guayule resin [3,4,8,11,13,[17][18][19] or latex [16] are scarce and all lacking of any validation protocol. Obviously, chromatographic methods are dominant in the analytical characterization of such analytes and, among them, HPLC is the preferred approach.…”

Section: Scheme 1 Structures Of Guayulins a And B (A) And Guayulins mentioning

confidence: 99%

Assessment, Validation and Application to Real Samples of a RP-HPLC Method for the Determination of Guayulins A, B, C and D in Guayule Shrub

Spano¹,

Meloni²,

Idda³

et al. 2018

Preprint

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Guayule (Parthenium argentatum Gray) is a shrub native of the arid regions of Mexico. In the last decades, significant attention for its cultivation has risen because it is the raw material for the production of hypoallergenic natural rubber. Guayule biomass contains also high amounts of resin, which is not normally exploited in any way. Among other sesquiterpenic esters, guayulins (i.e. the parteniol esters of cinnamic acid, guayulin A, or of anisic acid, guayulin B) are contained in resin. In addition, minor amounts of guayulin C and guayulin D are formed by degradation/oxidation of guayulins A and B, respectively. Guayulins likely act as cinnamate and p-anisate reservoirs for Guayule shrub, in addition, it has been postulated that they might have a key role in the chemical defense system of Guayule. Furthermore, it seems reasonable that guayulins can possess significant biological properties (e.g, antibacterial and anticancer activities), in close analogy with those shown by sesquiterpene lactones in many other species of Parthenum genus. As a matter of fact, guayulins A and B play an important role in the synthesis of antineoplastics used in breast cancer treatment. In this contribution we propose an original and validated RP-HPLC approach to the simultaneously quantification of guayulins A, B, C and D. The procedure of resin extraction from Guayule biomass has been optimized in terms of both nature of extraction method and solvent of extraction. RP-HPLC separation has been accomplished on an Ascentis® C18 column under isocratic elution with a 80:20 (v:v) acetonitrile:water mixture. Validation was carried out in terms of both the limit of detection, limit of quantification, linearity, precision, and trueness. Finally, the method was tested with a number of fresh and seasoned samples of spontaneous Guayule shrub from Mexico.

show abstract

“…For humans, the sensitizing power of guayulin A is much smaller than for laboratory animals. Hence, the possibility that traces of guayulin A may be present in the guayule rubber at a sensitizing level seems to be remote at the moment [16]. In analogy to what observed for sesquiterpene lactones in the most of the species of Parthenum genus, it is likely that guayulins may also exhibit other significant biological properties (i.e.…”

Section: Introductionmentioning

confidence: 85%

Assessment, Validation and Application to Real Samples of a RP-HPLC Method for the Determination of Guayulins A, B, C and D in Guayule Shrub

Spano¹,

Meloni²,

Idda³

et al. 2018

Preprint

View full text Add to dashboard Cite

Guayule (Parthenium argentatum Gray) is a shrub native of the arid regions of Mexico. In the last decades, significant attention for its cultivation has risen because it is the raw material for the production of hypoallergenic natural rubber. Guayule biomass contains also high amounts of resin, which is not normally exploited in any way. Among other sesquiterpenic esters, guayulins (i.e. the parteniol esters of cinnamic acid, guayulin A, or of anisic acid, guayulin B) are contained in resin. In addition, minor amounts of guayulin C and guayulin D are formed by degradation/oxidation of guayulins A and B, respectively. Guayulins likely act as cinnamate and p-anisate reservoirs for Guayule shrub, in addition, it has been postulated that they might have a key role in the chemical defense system of Guayule. Furthermore, it seems reasonable that guayulins may possess significant biological properties (e.g. antibacterial and anticancer activities), in close analogy with those shown by sesquiterpene lactones contained in many other species of Parthenum genus. As a matter of fact, guayulins A and B play an important role in the synthesis of antineoplastics used in breast cancer treatment. In this contribution we propose an original and validated RP-HPLC approach to the simultaneous quantification of guayulins A, B, C and D. The procedure of resin extraction from Guayule biomass has been optimized in terms of both extraction method and solvent. RP-HPLC separation has been accomplished by an Ascentis® C18 column under isocratic elution with a 80:20 (v:v) acetonitrile:water mixture. Validation was carried out in terms of limits of detection and quantification, linearity, precision, and trueness. Finally, the method was tested with a number of fresh and seasoned samples of spontaneous Guayule shrub from Mexico.

show abstract

ORIGINAL RESEARCH: Evaluation & control of potential sensitizing & irritating chemical components in natural rubber latex extracted from the industrial crop guayule

Cited by 15 publications

References 19 publications

Assessment, Validation and Application to Real Samples of an RP-HPLC Method for the Determination of Guayulins A, B, C and D in Guayule Shrub

Assessment, Validation and Application to Real Samples of an RP-HPLC Method for the Determination of Guayulins A, B, C and D in Guayule Shrub

Assessment, Validation and Application to Real Samples of a RP-HPLC Method for the Determination of Guayulins A, B, C and D in Guayule Shrub

Assessment, Validation and Application to Real Samples of a RP-HPLC Method for the Determination of Guayulins A, B, C and D in Guayule Shrub

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