Orthotopic Kidney Transplantation in the Rat With the Use of a Sleeve Arterial Anastomosis Method and a Modified Stenting Technique for Renal Veins

Jin, Zhong-da; Xue, Lingjie; Peng, Lan

doi:10.1016/j.transproceed.2017.04.022

Cited by 5 publications

(5 citation statements)

References 18 publications

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“… 16 – 18 Experimental models on animals are used today to perfect the ORT in the pursuit of reducing the warm ischemia time. 19 Despite the high promise of these new techniques and improvements, extensive research is needed to embed this techniques and improvements into the medical practice.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous living donor orthotopic renal transplantation and bilateral nephrectomy for recurrent renal cell carcinoma and renal failure: case report and review of literature

Novotný

Marada

Chlupáč

et al. 2018

RRU

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BackgroundWe report the case of a 43-year-old female patient with systemic lupus erythematosus, class III lupus nephritis, with predialysis creatinine levels around 350 μmol/L (3.95 mg/dL) after partial resection of the left kidney with histologically verified papillary carcinoma in 2010. Preoperative computed tomography of the abdomen revealed a small 8 mm tumor in the left upper kidney pole. The patient was indicated for simultaneous bilateral nephrectomy and orthotopic renal transplantation with the aim to minimize invasiveness of the procedure as well as for curable tumor removal.MethodThe procedure was performed under the full anesthesia trough upper middle laparotomy. As the first step, bilateral transperitoneal nephrectomy was performed. The live donor surgery started in a parallel theater to shorten the cold ischemic time of the graft. The renal graft had singe vessels and ureter; it was placed into the recipient’s right orthotopic position. End-to-end anastomosis of the right renal vein and artery anastomosis were performed; ureter was anastomosed end-to-end using recipient’s ureter.ResultsThe postoperative period was uneventful with repeatedly excellent ultrasonography check-up of the graft’s perfusion. The patient was discharged after 13 days with a good renal function of the graft (urea: 15 mmol/L, creatinine 160 μmol/L [1.80 mg/dL]).ConclusionOrthotopic renal transplantation is a technically challenging but valid alternative for patients who are unsuitable candidates for heterotopic renal transplantation or in cases where there is a clear benefit of orthotopic renal transplantation.

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Section: Discussionmentioning

confidence: 99%

Simultaneous living donor orthotopic renal transplantation and bilateral nephrectomy for recurrent renal cell carcinoma and renal failure: case report and review of literature

Novotný

Marada

Chlupáč

et al. 2018

RRU

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“…Then, the right kidney of the surviving donor rat is incised and transplanted to the left side of the second recipient (Group 4, n = 15). In all groups, renal artery (RA) revascularization was performed in an end-in-end fashion via modified sleeve anastomosis [ 3 , 4 ]. Renal vein (RV) anastomosis was performed end-to-end using a modified stenting technique [ 4 , 5 ].…”

Section: Methodsmentioning

confidence: 99%

“…In all groups, renal artery (RA) revascularization was performed in an end-in-end fashion via modified sleeve anastomosis [ 3 , 4 ]. Renal vein (RV) anastomosis was performed end-to-end using a modified stenting technique [ 4 , 5 ]. The ureter was anastomosed end-to-end using 6 interrupted equally spaced sutures.…”

Section: Methodsmentioning

confidence: 99%

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Optimized method to harvest both kidneys from one donor rat for transplantation

Xue

Cheng

et al. 2018

BMC Surg

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BackgroundRat renal transplantation is an essential experimental model for studies of transplantation immunobiology. Harvesting both kidneys from one donor rat for transplantation is widely used to reduce the number of experimental animals. Using the conventional method, both kidneys of the donor rat are harvested simultaneously, which leads to the prolonged warm ischemic times during transplantation of the second donor kidney. Prolonged warm ischemia time is the main risk factor for delayed graft function.MethodsTwo different approaches are compared. Method 1, conventional method: both kidneys of the donor rat are harvested simultaneously and then transplanted into two recipients. During transplantation, the first and second donor kidneys were regarded as Group 1 and 2, respectively. Method 2, step-by-step method: after left nephrectomy, the donor rat survives, and we perform left renal transplantation (Group 3). Then, the right kidney of the surviving donor rat is incised and transplanted into the left side of the second recipient (Group 4).ResultsThe success rates were 86.7, 93.3, 93.3 and 86.7% in groups 1, 2, 3 and 4, respectively. The warm ischemia times increased significantly in group 2 compared with the other 3 groups (p < 0.05) but differed non-significantly between groups 3 and 4 (p > 0.05). Serum creatinine levels, blood urea nitrogen and 24-h urine protein level obviously increased after kidney transplantation in group 2 compared with other groups (p < 0.05).ConclusionsWe developed an optimized method for reducing warm ischemia time, thereby minimizing delayed graft function.

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“…Although numerous researchers have endeavored to enhance anastomosis techniques, the complexity and intricacy of these methods may render them challenging to fully and accurately describe. Hence, there is an urgent need for a readily implementable and well-documented anastomosis technique, which could serve as a valuable reference for other rodent organ transplantation models, such as heart [ 16 ], liver [ 17 ], and kidney [ 18 ] transplantation.…”

Section: Introductionmentioning

confidence: 99%

A modified orthotopic left lung transplantation model in rats

Li,

Yu,

Dong

et al. 2024

Heliyon

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Orthotopic Kidney Transplantation in the Rat With the Use of a Sleeve Arterial Anastomosis Method and a Modified Stenting Technique for Renal Veins

Cited by 5 publications

References 18 publications

Simultaneous living donor orthotopic renal transplantation and bilateral nephrectomy for recurrent renal cell carcinoma and renal failure: case report and review of literature

Simultaneous living donor orthotopic renal transplantation and bilateral nephrectomy for recurrent renal cell carcinoma and renal failure: case report and review of literature

Optimized method to harvest both kidneys from one donor rat for transplantation

A modified orthotopic left lung transplantation model in rats

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