OsDCL3b affects grain yield and quality in rice

Liao, Pengfei; Ouyang, Jiexiu; Zhang, Jianjun; Yang, Lan; Wang, Xin; Peng, Xiaojue; Wang, Dong; Zhu, Youlin; Li, Shaobo

doi:10.1007/s11103-018-0806-x

Cited by 17 publications

(11 citation statements)

References 59 publications

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“…Unlike OsACBP2 which increases yield by enhancing grain weight, rice VIN3‐LIKE2 (OsVIL2) affected yield by suppressing cytokinin degradation, increasing grain number per panicle (Yang et al ., ). In contrast, knockdown of rice DICER‐LIKE3b ( OsDCL3b ) that functions in gene alternative splicing in rice panicles, reduced seed setting and impaired yield (Liao et al ., ). Interestingly, unlike OsACBP2‐OEs, there was no effect on grain weight in OsVIL2‐OE and OsDCL3b ‐RNAi lines (Liao et al ., ; Yang et al ., ).…”

Section: Discussionmentioning

confidence: 97%

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The overexpression of rice ACYL‐CoA‐BINDING PROTEIN2 increases grain size and bran oil content in transgenic rice

et al. 2019

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As Oryza sativa (rice) seeds represent food for over three billion people worldwide, the identification of genes that enhance grain size and composition is much desired. Past reports have indicated that Arabidopsis thaliana acyl-CoA-binding proteins (ACBPs) are important in seed development but did not affect seed size. Herein, rice OsACBP2 was demonstrated not only to play a role in seed development and germination, but also to influence grain size. OsACBP2 mRNA accumulated in embryos and endosperm of germinating seeds in qRT-PCR analysis, while b-glucuronidase (GUS) assays on OsACBP2pro::GUS rice transformants showed GUS expression in embryos, as well as the scutellum and aleurone layer of germinating seeds. Deletion analysis of the OsACBP2 5'-flanking region revealed five copies of the seed cis-element, Skn-I-like motif (À1486/À1482, À956/À952, À939/À935, À826/À822, and À766/À762), and the removal of any adversely affected expression in seeds, thereby providing a molecular basis for OsACBP2 expression in seeds. When OsACBP2 function was investigated using osacbp2 mutants and transgenic rice overexpressing OsACBP2 (OsACBP2-OE), osacbp2 was retarded in germination, while OsACBP2-OEs performed better than the wild-type and vector-transformed controls, in germination, seedling growth, grain size and grain weight. Transmission electron microscopy of OsACBP2-OE mature seeds revealed an accumulation of oil bodies in the scutellum cells, while confocal laser scanning microscopy indicated oil accumulation in OsACBP2-OE aleurone tissues. Correspondingly, OsACBP2-OE seeds showed gain in triacylglycerols and long-chain fatty acids over the vector-transformed control. As dietary rice bran contains beneficial bioactive components, OsACBP2 appears to be a promising candidate for enriching seed nutritional value.

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Section: Discussionmentioning

confidence: 97%

“…In contrast, knockdown of rice DICER‐LIKE3b ( OsDCL3b ) that functions in gene alternative splicing in rice panicles, reduced seed setting and impaired yield (Liao et al ., ). Interestingly, unlike OsACBP2‐OEs, there was no effect on grain weight in OsVIL2‐OE and OsDCL3b ‐RNAi lines (Liao et al ., ; Yang et al ., ).…”

Section: Discussionmentioning

confidence: 97%

The overexpression of rice ACYL‐CoA‐BINDING PROTEIN2 increases grain size and bran oil content in transgenic rice

et al. 2019

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“…The other report reported that there was another gene that affected the grain of rice, called OsDCL3b. Know down of this gene decreased grain yield, but it increased the quality of rice grain [47]. The other gene that was reported to affect the yield of rice was ARE1.…”

Section: Yield Componentsmentioning

confidence: 95%

West Sumatera Brown Rice Resistance to Fe

Dwipa

Suliansyah

Swasti

2019

Int. J. Adv. Sci. Eng. Inf. Technol.

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Brown rice is favored nutritious rice consumed by urban people whole the world as carbohydrate substitutes. This rice is appropriate for an urban community that concerns to a healthy lifestyle and diabetes people as the main staple food. One indicator of superior brown rice is resistant to abiotic stress, such as Fe. Fe is one of the main problems in cultivated land in Indonesia. The study aimed to study the West Sumatera brown rice resistance to Fe stress. West Sumatera belongs to the one province in Indonesia that can supply 2.5% of Indonesia rice requirement or 2 million tons in 2017. The research was conducted in the shade net house of Faculty of Agriculture, Andalas University, Padang, West Sumatera, Indonesia, from October 2017-January 2018. A completely randomized design was used in the assay and replied 3 times. 15 brown rice genotypes that consisted of 13 brown rice (Surian,

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“…A marked reduction of 21nt phasiRNAs was observed in the osdcl4-1 panicles [16]. The OsDCL3b RNAi lines led to reduced pollen fertility, seed setting rate and decreased grain yield in rice [44]. In addition, osdcl3b mutants specifically affected the generation of 24-nt phasiRNAs [16].…”

Section: Phas Loci Involved In Male Sterilitymentioning

confidence: 98%

Evolution of PHAS loci in the young spike of Allohexaploid wheat

Zhang

Huang

et al. 2020

BMC Genomics

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Background: PhasiRNAs (phased secondary siRNAs) play important regulatory roles in the development processes and biotic or abiotic stresses in plants. Some of phasiRNAs involve in the reproductive development in grasses, which include two categories, 21-nt (nucleotide) and 24-nt phasiRNAs. They are triggered by miR2118 and miR2275 respectively, in premeiotic and meiotic anthers of rice, maize and other grass species. Wheat (Triticum aestivum) with three closely related subgenomes (subA, subB and subD), is a model of allopolyploid in plants. Knowledge about the role of phasiRNAs in the inflorescence development of wheat is absent until now, and the evolution of PHAS loci in polyploid plants is also unavailable. Results: Using 261 small RNA expression datasets from various tissues, a batch of PHAS (phasiRNA precursors) loci were identified in the young spike of wheat, most of which were regulated by miR2118 and miR2275 in their target site regions. Dissection of PHAS and their trigger miRNAs among the diploid (AA and DD), tetraploid (AABB) and hexaploid (AABBDD) genomes of Triticum indicated that distribution of PHAS loci were dominant randomly in local chromosomes, while miR2118 was dominant only in the subB genome. The diversity of PHAS loci in the three subgenomes of wheat and their progenitor genomes (AA, DD and AABB) suggested that they originated or diverged at least before the occurrence of the tetraploid AABB genome. The positive correlation between the PHAS loci or the trigger miRNAs and the ploidy of genome indicated the expansion of genome was the major drive force for the increase of PHAS loci and their trigger miRNAs in Triticum. In addition, the expression profiles of the PHAS transcripts suggested they responded to abiotic stresses such as cold stress in wheat.Conclusions: Altogether, non-coding phasiRNAs are conserved transcriptional regulators that display quick plasticity in Triticum genome. They may be involved in reproductive development and abiotic stress in wheat. It could be referred to molecular research on male reproductive development in Triticum.

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OsDCL3b affects grain yield and quality in rice

Cited by 17 publications

References 59 publications

The overexpression of rice ACYL‐CoA‐BINDING PROTEIN2 increases grain size and bran oil content in transgenic rice

The overexpression of rice ACYL‐CoA‐BINDING PROTEIN2 increases grain size and bran oil content in transgenic rice

West Sumatera Brown Rice Resistance to Fe

Evolution of PHAS loci in the young spike of Allohexaploid wheat

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