OsDMC1 Is Not Required for Homologous Pairing in Rice Meiosis

Wang, Hongjun; Hu, Qing; Tang, Ding; Liu, Xiaofei; Du, Guijie; Shen, Yi; Li, Yafei; Cheng, Zhukuan

doi:10.1104/pp.16.00167

Cited by 69 publications

(70 citation statements)

References 63 publications

(82 reference statements)

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“…No significant difference in gH2AX signals was observed between meiocytes of zygo1 and the wild type at early prophase I ( Figures 5A and 5B), suggesting that the initiation of homologous recombination was not disturbed in zygo1. We also investigated the localizations of OsCOM1, OsDMC1, and OsZIP4, which are all essential elements involved in rice DSB repair (Ji et al, 2012;Shen et al, 2012;Wang et al, 2016). In the wild type, the signals of OsCOM1, OsDMC1, and OsZIP4 were observed as punctuate foci at zygotene.…”

Section: Zygo1 Is Not Required For Early Recombination Processesmentioning

confidence: 99%

“…The primary antibodies against OsREC8, PAIR2, PAIR3, g-H2AX, OsCOM1, OsDMC1, OsZIP4, and HEI10 were previously reported (Wang et al, 2011Ji et al, 2012;Shen et al, 2012;Wang et al, 2016). The guinea pig antibody against ZEP1 was generated with the ZEP1 fusion protein (amino acids 426-612) based on the report of the mouse antibody against ZEP1 (Wang et al, 2010).…”

Section: Antibody Productionmentioning

confidence: 99%

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The F-Box Protein ZYGO1 Mediates Bouquet Formation to Promote Homologous Pairing, Synapsis, and Recombination in Rice Meiosis

et al. 2017

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Telomere bouquet formation, a highly conserved meiotic event, plays an important role in homologous pairing and therefore progression of meiosis; however, the underlying molecular mechanism remains largely unknown. Here, we identified ZYGOTENE1 (ZYGO1), a novel F-box protein in rice (Oryza sativa), and verified its essential role in bouquet formation during early meiosis. In zygo1 mutants, zygotene chromosome aggregation and telomere clustering failed to occur. The suppressed telomere clustering in homologous pairing aberration in rice meiosis1 (pair1) zygo1 and rice completion of meiotic recombination (Oscom1) zygo1 double mutants, together with the altered localization of OsSAD1 (a SUN protein associated with the nuclear envelope) in zygo1, showed that ZYGO1 has a significant function in bouquet formation. In addition, the interaction between ZYGO1 and rice SKP1-like protein 1 suggested that ZYGO1 might modulate bouquet formation as a component of the SKP1-Cullin1-F-box complex. Although double-strand break formation and early recombination element installation occurred normally, zygo1 mutants showed defects in full-length pairing and synaptonemal complex assembly. Furthermore, crossover (CO) formation was disturbed, and foci of Human enhancer of invasion 10 were restricted to the partially synapsed chromosome regions, indicating that CO reduction might be caused by the failure of full-length chromosome alignment in zygo1. Therefore, we propose that ZYGO1 mediates bouquet formation to efficiently promote homolog pairing, synapsis, and CO formation in rice meiosis.

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Section: Zygo1 Is Not Required For Early Recombination Processesmentioning

confidence: 99%

Section: Antibody Productionmentioning

confidence: 99%

The F-Box Protein ZYGO1 Mediates Bouquet Formation to Promote Homologous Pairing, Synapsis, and Recombination in Rice Meiosis

et al. 2017

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“…OsCOM1 participates in meiotic DSB end resection, while OsDMC1 mediates the single-strand invasion in recombination (Ji et al, 2012;Wang et al, 2016). Both of these proteins are required for homologous recombination during meiosis in rice.…”

Section: Early Homologous Recombination Is Not Disturbed In Osrad1mentioning

confidence: 99%

“…Previous studies have shown that DMC1 mediates single-strand invasion and double Holliday junction formation during HR. Our previous study showed that OsDMC1A and DMC1B were functionally redundant in HR during rice meiosis (Wang et al, 2016). The situation of the Osdmc1a Osdmc1b double mutant was investigated.…”

Section: Osrad1 Functions In Parallel With Osrad51c and Osdmc1 Duringmentioning

confidence: 99%

The endonuclease homolog OsRAD1 promotes accurate meiotic double-strand break repair by suppressing non-homologous end joining

Tang

Wang

et al. 2016

Plant Physiol.

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“…In osmtopVIB-1 meiocytes, PAIR2 and PAIR3 loaded 57 normally onto the chromosomes and colocalized with OsREC8, which is consistent 58 with the wild type (Supplemental Figure 4). In the wild type, ZEP1 appeared as Rice DMC1 mediates single-strand invasion during meiotic recombination and is 67 essential for recombination dependent synapsis (Wang et al, 2016). MER3 is required 68 for the formation of interference-sensitive COs, the major pathway in rice (Wang et al,69 2009).…”

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confidence: 99%

The DNA Topoisomerase VI–B Subunit OsMTOPVIB Is Essential for Meiotic Recombination Initiation in Rice

Wang

Xue

et al. 2016

Molecular Plant

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In flowering plants, the life cycle alternates between diploid sporophyte and haploid 7 gametophyte generations. Development of the male organ and germ cells is essential 8 for successful plant fertility and crop yield. Male reproduction is a highly orchestrated 9 process controlled by various intrinsic components. Although significant advances 10 have been made in understanding male gametophyte formation, the molecular 11 mechanisms controlling in this process are still largely unknown (Zhang et al., 2016). 13To identify male sterility genes in rice, we have constructed a mutant library by 14 treating the rice cultivar 9522 (Oryza sativa ssp. Japonica) with gamma ray radiation. 15This has led to the identification of two allelic male sterile mutants that we have that OsMTOPVIB is essential for DSB formation in rice ( Figure 1D). and that pre-DSB sites originate in the chromosome loops before moving to the axes.

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OsDMC1 Is Not Required for Homologous Pairing in Rice Meiosis

Cited by 69 publications

References 63 publications

The F-Box Protein ZYGO1 Mediates Bouquet Formation to Promote Homologous Pairing, Synapsis, and Recombination in Rice Meiosis

The F-Box Protein ZYGO1 Mediates Bouquet Formation to Promote Homologous Pairing, Synapsis, and Recombination in Rice Meiosis

The endonuclease homolog OsRAD1 promotes accurate meiotic double-strand break repair by suppressing non-homologous end joining

The DNA Topoisomerase VI–B Subunit OsMTOPVIB Is Essential for Meiotic Recombination Initiation in Rice

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