OsDof25 expression alters carbon and nitrogen metabolism in Arabidopsis under high N-supply

Santos, Leandro Azevedo; Souza, Sônia Regina de; Fernandes, Mânlio Silvestre

doi:10.1007/s11816-012-0227-2

Cited by 44 publications

(27 citation statements)

References 43 publications

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“…Dof genes are reported to be involved in a wide range of plant biological processes, such as seed germination (Papi et al, 2000;Gualberti et al, 2002;Boccaccini et al, 2014), accumulation of storage proteins (Vicente-Carbajosa et al, 1997;Yamamoto et al, 2006;Dong et al, 2007) and lipids (Wang et al, 2007) in developing seeds, regulation of genes involved in plant nitrogen assimilation Rueda-L opez et al, 2008;Kurai et al, 2011;Santos et al, 2012), and carbon fixation (Yanagisawa, 2000;Tanaka et al, 2009;Kurai et al, 2011). Dof TFs also play important roles in the regulation of secondary metabolic processes, such as biosynthesis of glucosinolates and flavonoids (Skirycz et al, 2006(Skirycz et al, , 2007, and cell cycle regulation (Skirycz et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis and expression profiling of DNA-binding with one zinc finger (Dof) transcription factor family in potato

Venkatesh

Park

2015

Plant Physiology and Biochemistry

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Section: Introductionmentioning

confidence: 99%

Genome-wide analysis and expression profiling of DNA-binding with one zinc finger (Dof) transcription factor family in potato

Venkatesh

Park

2015

Plant Physiology and Biochemistry

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“…trichocarpa genome sequence by in silico analysis [55, 56]. The decrease in ammonium uptake in the transgenics could be explained by the regulation of some of these AMT genes in the plants overexpressing Dof5 as previously described [54,57]. …”

Section: Discussionmentioning

confidence: 98%

Overexpression of a pine Dof transcription factor in hybrid poplars: A comparative study in trees growing under controlled and natural conditions

et al. 2017

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In this work, the role of the pine transcriptional regulator Dof 5 in carbon and nitrogen metabolism has been examined in poplar trees. The overexpression of the gene and potential effects on growth and biomass production were compared between trees growing in a growth chamber under controlled conditions and trees growing in a field trial during two growth seasons. Ten-week-old transgenic poplars exhibited higher growth than untransformed controls and exhibited enhanced capacity for inorganic nitrogen uptake in the form of nitrate. Furthermore, the transgenic trees accumulated significantly more carbohydrates such as glucose, fructose, sucrose and starch. Lignin content increased in the basal part of the stem likely due to the thicker stem of the transformed plants. The enhanced levels of lignin were correlated with higher expression of the PAL1 and GS1.3 genes, which encode key enzymes involved in the phenylalanine deamination required for lignin biosynthesis. However, the results in the field trial experiment diverged from those observed in the chamber system. The lines overexpressing PpDof5 showed attenuated growth during the two growing seasons and no modification of carbon or nitrogen metabolism. These results were not associated with a decrease in the expression of the transgene, but they can be ascribed to the nitrogen available in the field soil compared to that available for growth under controlled conditions. This work highlights the paramount importance of testing transgenic lines in field trials.

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“…DAG1 (DOF AFFECTING GERMINATION 1) is involved in seed germination in Arabidopsis (Papi et al ., ). In rice ( Oryza sativa ), OsDOF4 and OsDOF12 participate in flowering time regulation (Li et al ., ; Wu et al ., ), and OsDOF24 and OsDOF25 are involved in carbon and nitrogen metabolism (Santos et al ., ; Zhang et al ., ; Wu et al ., ; Yu et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Rice OsDOF15 contributes to ethylene‐inhibited primary root elongation under salt stress

et al. 2019

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Summary In early seedlings, the primary root adapts rapidly to environmental changes through the modulation of endogenous hormone levels. The phytohormone ethylene inhibits primary root elongation, but the underlying molecular mechanism of how ethylene‐reduced root growth is modulated in environmental changes remains poorly understood. Here, we show that a novel rice (Oryza sativa) DOF transcription factor OsDOF15 positively regulates primary root elongation by regulating cell proliferation in the root meristem, via restricting ethylene biosynthesis. Loss‐of‐function of OsDOF15 impaired primary root elongation and cell proliferation in the root meristem, whereas OsDOF15 overexpression enhanced these processes, indicating that OsDOF15 is a key regulator of primary root elongation. This regulation involves the direct interaction of OsDOF15 with the promoter of OsACS1, resulting in the repression of ethylene biosynthesis. The control of ethylene biosynthesis by OsDOF15 in turn regulates cell proliferation in the root meristem. OsDOF15 transcription is repressed by salt stress, and OsDOF15‐mediated ethylene biosynthesis plays a role in inhibition of primary root elongation by salt stress. Thus, our data reveal how the ethylene‐inhibited primary root elongation is finely controlled by OsDOF15 in response to environmental signal, a novel mechanism of plants responding to salt stress and transmitting the information to ethylene biosynthesis to restrict root elongation.

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OsDof25 expression alters carbon and nitrogen metabolism in Arabidopsis under high N-supply

Cited by 44 publications

References 43 publications

Genome-wide analysis and expression profiling of DNA-binding with one zinc finger (Dof) transcription factor family in potato

Genome-wide analysis and expression profiling of DNA-binding with one zinc finger (Dof) transcription factor family in potato

Overexpression of a pine Dof transcription factor in hybrid poplars: A comparative study in trees growing under controlled and natural conditions

Rice OsDOF15 contributes to ethylene‐inhibited primary root elongation under salt stress

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