Osmanicin, a Polyketide Alkaloid Isolated from Streptomyces osmaniensis CA-244599 Inhibits Elastase in Human Fibroblasts

Samy, Mamdouh Nabil; Goff, Géraldine Le; Lopes, Philippe; Georgousaki, K; Gumeni, Sentiljana; Almeida, Celso; González, Ignacio; Genilloud, Olga; Trougakos, Ioannis P.; Fokialakis, Nikolas; Ouazzani, Jamal

doi:10.3390/molecules24122239

Cited by 10 publications

(6 citation statements)

References 38 publications

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“…S. osmaniensis OU-63 was firstly isolated from garden soil samples on the campus of Osmania University, Hyderabad, India (Reddy et al 2010). Samy et al (2019) succeeded in isolating S. osmaniensis CA-244599 from soil samples in a savanna environment in Bangouamafsakoa, in the Comoros Islands. S. osmaniensis CA-244599 is known to be able to produce osmanicin compounds, streptazoline, streptazone C, streptazone B1, streptenol, nocardamine, and desmethylenyl-nocardamine.…”

Section: Discussionmentioning

confidence: 99%

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The presence of endophytic actinobacteria in mangosteen peel (Garcinia mangostana) and its antioxidant activity

2020

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Abstract. Larasati F, Batubara I, Lestari Y. 2020. The presence of endophytic actinobacteria in mangosteen peel (Garcinia mangostana L.) and its antioxidant activity. Biodiversitas 21: 1488-1497. Mangosteen (Garcinia mangostana L.) is a family member of Clusiaceae which is rich in secondary metabolite compounds that can function as antioxidants. Besides being produced by its host plant, the bioactive compounds can also be produced by endophytic actinobacteria. The purpose of this study was to explore the presence of endophytic actinobacteria from mangosteen peel and determine its antioxidant activity. The actinobacteria were isolated, purified, morphologically characterized, molecularly identified, extracted with ethyl acetate and tested for antioxidant properties. The antioxidant activity was assayed using DPPH (2.2-diphenyl-1-picrylhydrazyl) and ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) methods. The components of extracts were separated by Thin Layer Chromatography (TLC) and bioautography was done to determine the antioxidant bands. As a result, five isolates of endophytic actinobacteria in mangosteen peel showed to have difference in aerial mycelium color, substrate mycelium color, and types of spore chains. Based on 16S rRNA gene analysis, AGM3.2 isolate showed similarity with Streptomyces griseochromogenes ATCC 14511 (T) 99.06%. AGM3.1 had similarity with Streptomyces osmaniensis OU-63 (T) 98.35%. Meanwhile, AGM2.3 were similar to Streptomyces xanthophaeus NBRC B-5414 (T) 99.82%, AGM2.2 had similarity with Streptomyces xanthophaeus NBRC B-5414 (T) 98.95%. In addition, AGM2.1 has homology with Streptomyces goshikiensis NBRC 12868 (T) 99.52%. Using both DPPH and ABTS, supernatant of AGM2.1 showed the highest antioxidant activity indicated by 36.96 and 98.80 inhibition, respectively. Antioxidant capacity of ethyl acetate extract of AGM2.1 was 22.22 μg AEAC/mg extract (DPPH) and 20.34 μg AEAC/mg extract (ABTS). Meanwhile, ethyl acetate extract of mangosteen peel had antioxidant capacity by 21.17 µg AEAC/mg extract (DPPH) and 18.75 µg AEAC/mg extract (ABTS). Antioxidant bioautographic analysis of mangosteen peel ethyl acetate extract was compared with alpha mangosteen standard. The results showed that alpha mangosteen presence in the mangosteen extract with the same Rf value of 0.64 with standard. Meanwhile, actinobacterial ethyl acetate extract from AGM3.1, AGM2.3, AGM2.2, AGM2.1 each have the same Rf value with the alpha mangosteen standard. However, the spot for alpha mangosteen had dark red color, while spots of the four actinobacterial isolates showed to have blue color indicating different antioxidant compounds. The blue spot indicates the flavone, flavanone, flavonol, and isoflavone. These compounds include a subgroup of flavonoid compounds. Ethyl acetate extract AGM3.2 does not have spot compounds with the same Rf value as the alpha mangosteen standard. Study clearly shows that endophytic actinobacteria from mangosteen peel have potency as antioxidant.

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Section: Discussionmentioning

confidence: 99%

“…It is known that osmanicin has the strongest activity with IC50 value of 3.7 µM. Osmanicin compounds are known to be effective in antiaging treatment of the skin (Samy et al 2019). AGM3.2 isolate had similarity with S. griseochromogenes of 99.06%.…”

Section: Discussionmentioning

confidence: 99%

The presence of endophytic actinobacteria in mangosteen peel (Garcinia mangostana) and its antioxidant activity

2020

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“…As a hotspot of biodiversity, the Comoros Archipelago, situated off the southeastern coast of Africa, in the Mozambique channel, is poorly explored for natural products chemistry [ 2 ]. Hence, the archipelago represents a great potential for the discovery of original biologically active metabolites [ 3 , 4 ]. In the course of our research aimed at the discovery of bioactive metabolites with potential application in the treatment of age-related diseases and skin aging such as anti-CDK7, anti-tyrosinase or sirtuin 1 activators substances, 44 marine sponges were collected from the lagoon of Mayotte and evaluated.…”

Section: Introductionmentioning

confidence: 99%

New Metabolites from the Marine Sponge Scopalina hapalia Collected in Mayotte Lagoon

Hassane¹,

Herbette

Garayev

et al. 2022

Marine Drugs

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The biological screening of 44 marine sponge extracts for the research of bioactive molecules, with potential application in the treatment of age-related diseases (cancer and Alzheimer’s disease) and skin aging, resulted in the selection of Scopalina hapalia extract for chemical study. As no reports of secondary metabolites of S. hapalia were found in the literature, we undertook this research to further extend current knowledge of Scopalina chemistry. The investigation of this species led to the discovery of four new compounds: two butenolides sinularone J (1) and sinularone K (2), one phospholipid 1-O-octadecyl-2-pentanoyl-sn-glycero-3-phosphocholine (3) and one lysophospholipid 1-O-(3-methoxy-tetradecanoyl)-sn-glycero-3-phosphocholine (4) alongside with known lysophospholipids (5 and 6), alkylglycerols (7–10), epidioxysterols (11 and 12) and diketopiperazines (13 and 14). The structure elucidation of the new metabolites (1–4) was determined by detailed spectroscopic analysis, including 1D and 2D NMR as well as mass spectrometry. Molecular networking was also explored to complement classical investigation and unravel the chemical classes within this species. GNPS analysis provided further information on potential metabolites with additional bioactive natural compounds predicted.

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“…Among various concentration techniques, Amberlite XAD resin trapping of organic compounds gained recent interest and finds application in plant [ 5 , 6 ] marine invertebrate [ 7 , 8 ], and microbial [ 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 ] specialized metabolite extraction.…”

Section: Introductionmentioning

confidence: 99%

“…We have recently extended the exploitation of in-situ XAD-16 extraction of mycelium forming microorganisms, actinomycetes and fungi [ 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 ], known as the major providers of valuable bioactive compounds [ 19 , 20 ]. Compared to submerged cultivation, we showed that in-situ XAD-16 extraction significantly impacts the diversity and the yields of target compounds [ 9 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Solid-Phase Extraction Embedded Dialysis (SPEED), an Innovative Procedure for the Investigation of Microbial Specialized Metabolites

et al. 2021

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Solid-phase extraction embedded dialysis (SPEED technology) is an innovative procedure developed to physically separate in-situ, during the cultivation, the mycelium of filament forming microorganisms, such as actinomycetes and fungi, and the XAD-16 resin used to trap the secreted specialized metabolites. SPEED consists of an external nylon cloth and an internal dialysis tube containing the XAD resin. The dialysis barrier selects the molecular weight of the trapped compounds, and prevents the aggregation of biomass or macromolecules on the XAD beads. The external nylon promotes the formation of a microbial biofilm, making SPEED a biofilm supported cultivation process. SPEED technology was applied to the marine Streptomyces albidoflavus 19-S21, isolated from a core of a submerged Kopara sampled at 20 m from the border of a saltwater pond. The chemical space of this strain was investigated effectively using a dereplication strategy based on molecular networking and in-depth chemical analysis. The results highlight the impact of culture support on the molecular profile of Streptomyces albidoflavus 19-S21 secondary metabolites.

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Osmanicin, a Polyketide Alkaloid Isolated from Streptomyces osmaniensis CA-244599 Inhibits Elastase in Human Fibroblasts

Cited by 10 publications

References 38 publications

The presence of endophytic actinobacteria in mangosteen peel (Garcinia mangostana) and its antioxidant activity

The presence of endophytic actinobacteria in mangosteen peel (Garcinia mangostana) and its antioxidant activity

New Metabolites from the Marine Sponge Scopalina hapalia Collected in Mayotte Lagoon

Solid-Phase Extraction Embedded Dialysis (SPEED), an Innovative Procedure for the Investigation of Microbial Specialized Metabolites

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