Osmotic-shock produced by vitrification solutions improves immature human oocytes in vitro maturation

Molina, Inmaculada; Gómez, J. Gallego; Balasch, S.; Pellicer, Nuria; Novella-Maestre, Edurne

doi:10.1186/s12958-016-0161-1

Cited by 24 publications

(22 citation statements)

References 46 publications

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“…For oocyte maturation after IVM, it is important to evaluate the quality of the oocyte at various points; however, several aspects of this process remain to be elucidated. Nuclear maturation can be evaluated based on the form of the germinal vesicle (GV) and extrusion of the first polar body, but evaluation of cytoplasmic maturation is difficult . As with nuclear maturation, it is not clear whether the cytoplasm changes dramatically in the IVM process .…”

Section: Maturation Of Oocytes After In Vitro Maturation For Fertilitmentioning

confidence: 99%

“…When considering the results of cryopreservation of the oocytes in IVM, it is necessary to pay attention to whether the reference literature performed the slow‐freezing method or vitrification . Recent reports have not concluded whether it is preferable to cryopreserve immature oocytes at the GV or MII stage . Vitrification results in more normally formed spindles than the slow‐freezing method .…”

Section: Cryopreservation Of Oocytes For Fertility Preservationmentioning

confidence: 99%

“…In Figure , the resected ovaries of a patient with an endometrioid adenocarcinoma are shown, as well as the collected immature oocytes and mature oocytes after IVM. However, it is difficult to fertilize mature oocytes that have been obtained after IVM due to legal and ethical concerns . In addition, if the fertilization process or the development of the blastocyst cannot be evaluated, it is difficult to fully evaluate the oocyte's potential.…”

Section: Recent Research On Fertility Preservation Using In Vitro Matmentioning

confidence: 99%

“…This is a limitation of research on IVM and it is necessary to recognize that there is also a limitation on the provision of information to patients who are undergoing FP. Recently, as a substitute for the fertilization process, several researchers have studied parthenogenetic embryonic development in oocytes after IVM . Currently, it is necessary to evaluate fertilization and the development of blastocysts by simulation, to the extent that this is possible.…”

Section: Recent Research On Fertility Preservation Using In Vitro Matmentioning

confidence: 99%

“…Nuclear maturation can be evaluated based on the form of the germinal vesicle (GV) and extrusion of the first polar body, but evaluation of cytoplasmic maturation is difficult. 61 As with nuclear maturation, it is not clear whether the cytoplasm changes dramatically in the IVM process. 7,62 Furthermore, it is not known whether oocytes that have been matured by IVM are equivalent to oocytes that have developed in vivo by conventional ART and there is some evidence that the IVM oocytes might be inferior, from the standpoint of their developmental potential.…”

Section: T a B L E 3 Reports Of In Vitro Maturation For Fertility Prementioning

confidence: 99%

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In vitro maturation of human immature oocytes for fertility preservation and research material

Shirasawa

Terada

2017

Reprod Medicine & Biology

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AimIn recent years, the importance of fertility preservation (FP) has increased. In vitro maturation (IVM), an important technique in FP, has started to be used in the clinic, but controversies persist regarding this technique. Here, a survey of IVM for FP is provided.MethodsBased on a literature review, the applications of FP, methods of FP, IVM of oocytes that had been collected in vivo and ex vivo, maturation of oocytes after IVM for FP, cryopreservation of oocytes for FP, explanation of the procedures to patients, and recent research on FP using IVM were investigated.ResultsAlthough IVM for FP remains controversial, the application of FP is expected to expand. Depending on the age and disease status of the patient, various methods of oocyte collection and ovarian stimulation, as well as various needle types and aspiration pressures, have been reported. The maturation rate of IVM in FP ranges widely and requires optimization in the future. In regard to cryopreservation for matured oocytes, the vitrification method is currently recommended.ConclusionRegarding FP for patients with cancer, the treatment of cancer is prioritized; thus, the time and use of medicines are often constrained. As several key points regarding IVM remain unclear, well‐designed and specific counseling for patients is necessary.

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Section: Maturation Of Oocytes After In Vitro Maturation For Fertilitmentioning

confidence: 99%

Section: Cryopreservation Of Oocytes For Fertility Preservationmentioning

confidence: 99%

Section: Recent Research On Fertility Preservation Using In Vitro Matmentioning

confidence: 99%

Section: Recent Research On Fertility Preservation Using In Vitro Matmentioning

confidence: 99%

Section: T a B L E 3 Reports Of In Vitro Maturation For Fertility Prementioning

confidence: 99%

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In vitro maturation of human immature oocytes for fertility preservation and research material

Shirasawa

Terada

2017

Reprod Medicine & Biology

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Vitrification of human immature oocytes before and after in vitro maturation: a review

Khalili

Shahedi

Ashourzadeh

et al. 2017

J Assist Reprod Genet

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The use of immature oocytes subjected to in vitro maturation (IVM) opens interesting perspectives for fertility preservation where ovarian reserves are damaged by pathologies or therapies, as in PCO/PCOS and cancer patients. Human oocyte cryopreservation may offer some advantages compared to embryo freezing, such as fertility preservation in women at risk of losing fertility due to oncological treatment or chronic disease, egg donation and postponing childbirth. It also eliminates religious and/or other ethical, legal, and moral concerns of embryo freezing. In addition, a successful oocyte cryopreservation program could eliminate the need for donor and recipient menstrual cycle synchronization. Recent advances in vitrification technology have markedly improved the oocyte survival rate after warming, with fertilization and implantation rates comparable with those of fresh oocytes. Healthy live births can be achieved from the combination of IVM and vitrification, even if vitrification of in vivo matured oocytes is still more effective. Recently, attention is given to highlight whether vitrification procedures are more successful when performed before or after IVM, on immature GV-stage oocytes, or on in vitro matured MII-stage oocytes. In this review, we emphasize that, even if there are no differences in survival rates between oocytes vitrified prior to or post-IVM, reduced maturation rates of immature oocytes vitrified prior to IVM can be, at least in part, explained by underlying ultrastructural and biomolecular alterations.

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Vitrification of Human Oocytes Before or After Rescue-IVM Does not Impair Maturation Kinetics but Induces Meiotic Spindle Alterations

Marteil,

Metchat,

Dollet

et al. 2024

Reprod. Sci.

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Cryopreservation of in vitro matured oocytes is still considered as an experimental alternative to mature oocyte vitrification after ovarian stimulation. Here, we investigated whether rescue-IVM should be performed before or after vitrification. For this, 101 immature oocytes (germinal vesicle stage) from women undergoing ICSI were used. Oocytes were divided into three groups: freshly in vitro matured oocytes (IVM), freshly in vitro matured oocytes subsequently vitrified (IVM + VIT) and vitrified/warmed GV oocytes then in vitro matured (VIT + IVM). Oocyte maturation rates and kinetics were assessed using time-lapse technology. Spindle dimensions and polarity, chromosome alignment and cytoplasmic F-actin filament length and density were determined using confocal microscopy and quantitative image analyses. No differences in IVM rates (fresh IVM: 63.16% and IVM post-VIT: 59.38%, p = 0.72) and timings (17.73 h in fresh IVM, 17.33 h in IVM post-VIT, p = 0.72) were observed whether IVM is performed freshly or after vitrification. Meiotic spindles were shorter in VIT + IVM (10.47 µm vs 11.23 µm in IVM and 11.40 µm in IVM + VIT, p = 0.012 and p = 0.043) and wider in IVM + VIT (9.37 µm vs 8.12 µm in IVM and 8.16 µm VIT + IVM, p = 0.027 and p = 0.026). The length-to-width ratio was lower in vitrified groups (IVM + VIT: 1.19 and VIT + IVM: 1.26) compared to IVM (1.38), p = 0.013 and p = 0.014. No differences in multipolar spindle and chromosome misalignment occurrence and cytoplasmic F-actin filament length and density were observed between groups. Our results suggest vitrification before or after rescue-IVM does not seem to impair maturation rates and kinetics parameters but induces meiotic spindle alterations.

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Osmotic-shock produced by vitrification solutions improves immature human oocytes in vitro maturation

Cited by 24 publications

References 46 publications

In vitro maturation of human immature oocytes for fertility preservation and research material

In vitro maturation of human immature oocytes for fertility preservation and research material

Vitrification of human immature oocytes before and after in vitro maturation: a review

Vitrification of Human Oocytes Before or After Rescue-IVM Does not Impair Maturation Kinetics but Induces Meiotic Spindle Alterations

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