Osteoblast and Osteoclast Activity Affect Bone Remodeling Upon Regulation by Mechanical Loading-Induced Leukemia Inhibitory Factor Expression in Osteocytes

Du, Jingke; Yang, Juhua; He, Zihao; Cui, Junqi; Yang, Yiqi; Xu, Mingming; Qu, Xinhua; Zhao, Ning; Yan, Mi; Li, Hanjun; Yu, Zhifeng

doi:10.3389/fmolb.2020.585056

Cited by 23 publications

(13 citation statements)

References 44 publications

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“…LIF secretion by osteocytes can increase the tartrate-resistant acid phosphatase activity of BMM-derived osteoclasts and enhance alkaline phosphatase activity during the osteogenic differentiation of BMSCs. Mechanistically, LIF increases the phosphorylation of STAT3, which is associated with osteoclast and osteoblast formation [ 108 ] (Fig. 5 ).…”

Section: Cell–cell Interactions Via Stat3 Activationmentioning

confidence: 99%

STAT3-mediated osteogenesis and osteoclastogenesis in osteoporosis

Hou

Tian

2022

Cell Commun Signal

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Osteoporosis is a common skeletal disease with marked bone loss, deterioration of the bone microstructure and bone fragility. An abnormal bone remodelling cycle with relatively increased bone resorption is the crucial pathophysiological mechanism. Bone remodelling is predominantly controlled by osteoblasts and osteoclasts, which are specialized cell types that are regulated by a variety of osteogenic and osteoclastic factors, including cytokines expressed within the bone microenvironment under local or systemic inflammatory conditions. Signal transducer and activator of transcription 3 (STAT3) plays a prominent role in the communication between cytokines and kinases by binding downstream gene promotors and is involved in a wide range of biological or pathological processes. Emerging evidence suggests that STAT3 and its network participate in bone remodelling and the development of osteoporosis, and this factor may be a potent target for osteoporosis treatment. This review focuses on the role and molecular mechanism of the STAT3 signalling pathway in osteogenesis, osteoclastogenesis and osteoporosis, particularly the bone-related cytokines that regulate the osteoblastic differentiation of bone marrow stromal cells and the osteoclastic differentiation of bone marrow macrophages by initiating STAT3 signalling. This review also examines the cellular interactions among immune cells, haematopoietic cells and osteoblastic/osteoclastic cells.

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Section: Cell–cell Interactions Via Stat3 Activationmentioning

confidence: 99%

STAT3-mediated osteogenesis and osteoclastogenesis in osteoporosis

Hou

Tian

2022

Cell Commun Signal

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“…A recent study showed that the leukemia inhibitory factor (LIF) enhanced signal transducer and activator of transcription 3 (STAT3) phosphorylation in BMSCs and increased the expression of ALP and osteogenic genes. However, in the in vitro microgravity environment, the secretion of LIF by bone cells was inhibited, which weakened the osteogenic effect ( Du et al, 2020 ). Microgravity also increases the level of oxidative damage markers in the body and weakens the total antioxidant capacity.…”

Section: Mechanical Stimulation With Osteoblastsmentioning

confidence: 99%

Effects of Mechanical Stress Stimulation on Function and Expression Mechanism of Osteoblasts

Liu

Wang

et al. 2022

Front. Bioeng. Biotechnol.

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Osteoclasts and osteoblasts play a major role in bone tissue homeostasis. The homeostasis and integrity of bone tissue are maintained by ensuring a balance between osteoclastic and osteogenic activities. The remodeling of bone tissue is a continuous ongoing process. Osteoclasts mainly play a role in bone resorption, whereas osteoblasts are mainly involved in bone remodeling processes, such as bone cell formation, mineralization, and secretion. These cell types balance and restrict each other to maintain bone tissue metabolism. Bone tissue is very sensitive to mechanical stress stimulation. Unloading and loading of mechanical stress are closely related to the differentiation and formation of osteoclasts and bone resorption function as well as the differentiation and formation of osteoblasts and bone formation function. Consequently, mechanical stress exerts an important influence on the bone microenvironment and bone metabolism. This review focuses on the effects of different forms of mechanical stress stimulation (including gravity, continuously compressive pressure, tensile strain, and fluid shear stress) on osteoclast and osteoblast function and expression mechanism. This article highlights the involvement of osteoclasts and osteoblasts in activating different mechanical transduction pathways and reports changings in their differentiation, formation, and functional mechanism induced by the application of different types of mechanical stress to bone tissue. This review could provide new ideas for further microscopic studies of bone health, disease, and tissue damage reconstruction.

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“…Leukemia inhibitory factor (LIF) is a member of the interleukin-6 family of cytokines with highly pleiotropic biological activities. LIF expression has been reported for all three main bone cells by different groups [ 55 ]. Recently, LIF was shown to be released into the blood at high levels of mechanical stimulation; conversely, its bone and serum levels decrease in tail-suspended mice, as well as in MLO-Y4 cells grown under random positioning machine (RPM) [ 55 ].…”

Section: Recent Updates On the Responses Of Osteocytes To Weightlessness At The Cellular And Molecular Levelmentioning

confidence: 99%

“…LIF expression has been reported for all three main bone cells by different groups [ 55 ]. Recently, LIF was shown to be released into the blood at high levels of mechanical stimulation; conversely, its bone and serum levels decrease in tail-suspended mice, as well as in MLO-Y4 cells grown under random positioning machine (RPM) [ 55 ]. Khosla’s group identified LIF as one of the factors present in both serum and bone whose levels decrease in post-menopausal women.…”

Section: Recent Updates On the Responses Of Osteocytes To Weightlessness At The Cellular And Molecular Levelmentioning

confidence: 99%

“…RT-PCR analysis shows that MLO-Y4 subjected to simulated microgravity express higher levels of the pro-apoptotic marker Bax and Bad [ 55 ], providing some, though by far not conclusive, evidence that unloading induced apoptosis occurs in vitro under simulated microgravity conditions. Obviously, it is hard to unequivocally attribute programmed cell death to microgravity as opposed to sub-optimal culture conditions.…”

Section: Is Space-flight Bone Loss Linked To Osteocyte Apoptosis or Senescence?mentioning

confidence: 99%

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Osteocytes and Weightlessness

Iandolo

Strigini

Guignandon

et al. 2021

Curr Osteoporos Rep

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Purpose of Review Osteocytes are considered to be the cells responsible for mastering the remodeling process that follows the exposure to unloading conditions. Given the invasiveness of bone biopsies in humans, both rodents and in vitro culture systems are largely adopted as models for studies in space missions or in simulated microgravity conditions models on Earth. Recent Findings After a brief recall of the main changes in bone mass and osteoclastic and osteoblastic activities in space-related models, this review focuses on the potential role of osteocytes in directing these changes. The role of the best-known signalling molecules is questioned, in particular in relation to osteocyte apoptosis. Summary The mechanotransduction actors identified in spatial conditions and the problems related to fluid flow and shear stress changes, probably enhanced by the alteration in fluid flow and lack of convection during spaceflight, are recalled and discussed.

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Osteoblast and Osteoclast Activity Affect Bone Remodeling Upon Regulation by Mechanical Loading-Induced Leukemia Inhibitory Factor Expression in Osteocytes

Cited by 23 publications

References 44 publications

STAT3-mediated osteogenesis and osteoclastogenesis in osteoporosis

STAT3-mediated osteogenesis and osteoclastogenesis in osteoporosis

Effects of Mechanical Stress Stimulation on Function and Expression Mechanism of Osteoblasts

Osteocytes and Weightlessness

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