Osteoblasts proliferation and differentiation stimulating activities of the main components of Fructus Psoraleae corylifoliae

Li, Wei D.; Cui, Yan; Wu, Yu; Weng, Zebin; Yin, Fei; Yang, Guang; Cai, Bao Chang; Chen, Zhi P.

doi:10.1016/j.phymed.2013.09.015

Cited by 99 publications

(59 citation statements)

References 27 publications

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“…Total flavones of Epimedium (TFE) inhibited reduction of procollagen type I N-terminal propeptide (PINP), and increased serum osteocalcin and type I collagen in OVX rats (13,14). Icariin, one of the major components of Herba Epimedium, decreased activities of serum tartrate-resistant acid phosphatase (TRAP) and bioactive components could stimulate the proliferation, differentiation and mineralization of osteoblasts (12,(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40), suppress the adipogenesis of bone marrow-derived mesenchymal stem cells (BMSCs) (41)(42)(43)(44), inhibit the proliferation and differentiation of osteoclasts (45)(46)(47), and induce apoptosis and cell cycle arrest and suppress bone resorption of osteoclasts (48). In addition, icariin was found to significantly attenuate oxidative stress and apoptosis and preserve viability and osteogenic potential of osteoblasts exposed to hypoxia, which indicated that its anti-osteoporotic effect might be attributed to its anti-hypoxic activity (49).…”

Section: In Vivo Findingsmentioning

confidence: 99%

Chinese single herbs and active ingredients for postmenopausal osteoporosis: From preclinical evidence to action mechanism

Lin

Wang

Zhang

et al. 2017

BST

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Section: In Vivo Findingsmentioning

confidence: 99%

Chinese single herbs and active ingredients for postmenopausal osteoporosis: From preclinical evidence to action mechanism

Lin

Wang

Zhang

et al. 2017

BST

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“…Previous studies have demonstrated the effect of psoralen on osteoblast proliferation in vitro (29,30). To elucidate the mechanism of action of psoralen on osteoblast proliferation, the effects of psoralen on three primary signaling pathways, namely extracellular signal-related kinase (ERK), c-Jun N-terminal kinase (JNK) and MAPK, were determined by evaluating signal pathway activity and cell proliferation (Fig.…”

Section: Hfob119 Cell Proliferation Depends On Nk-κb-mapk Signalingmentioning

confidence: 99%

Psoralen stimulates osteoblast proliferation through the activation of nuclear factor-κB-mitogen-activated protein kinase signaling

Feimeng

Huang³

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. Osteoporosis is a systemic skeletal disease that leads to increased bone fragility and susceptibility to fracture. Approximately 50% of postmenopausal women develop osteoporosis as a result of postmenopausal estrogen deficiency. To reduce fractures related to osteoporosis in women, previous studies have focused on therapeutic strategies that aim to increase bone formation or decrease bone resorption. However, pharmacological agents that aim to improve bone fracture susceptibility exhibit side effects. Current studies are investigating natural alternatives that possess the benefits of selective estrogen receptor modulators (SERMs) without the adverse effects. Recent studies have indicated that phytoestrogen may be an ideal natural SERM for the treatment of osteoporosis. In Chinese herbal medicine, psoralen, as the predominant substance of Psoralea corylifolia, is considered to be a phytoestrogen and is used as a remedy for osteoporosis. A number of studies have demonstrated the efficacy of psoralen in bone formation. However, the pathways and underlying molecular mechanisms that participate in psoralen-induced osteoblast formation are not well understood. In the present study, hFOB1.19 cells were treated with psoralen at different concentrations (0, 5, 10, 15 and 20 µM) for 0, 24, 36, 48 and 72 h, respectively. Reverse transcription-quantitative polymerase chain reaction and western blot assays were performed to detect glucose transporter 3 (GLUT3) expression. A cell counting kit-8 assay was used to analyze cell proliferation. In addition the effects of mitogen activated protein kinase inhibitors on extracellular signal-regulated kinase (ERK), phosphorylated (p)-ERK, p38, p-p38, c-Jun N-terminal kinase (JNK) and p-JNK expressions and cell proliferation were measured, as was the effect of nuclear factor (NF)-κB inhibitor on P65 and GLUT3 expressions and cell proliferation. The results indicated that psoralen stimulates hFOB1.19 cell proliferation in a dose-dependent manner (P<0.05). Phospho-ERK, p38 and JNK were markedly increased by psoralen compared with the control group (P<0.05), and the specific inhibitors of ERK (SCH772984), p38 (SB203580) and JNK (SP600125) reversed the stimulatory effects of psoralen on signal marker phosphorylation (P<0.05). The rate of psoralen-induced cell proliferation was significantly suppressed by inhibitors of ERK, JNK and p38 compared with psoralen treatment alone (P<0.05). In addition, psoralen stimulated osteoblast proliferation via the NF-κB signaling pathway. Therefore, the present findings suggest that psoralen may be a potential natural alternative to SERMs in the treatment of osteoporosis and fractures.

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“…Dry and ripe seeds of leguminous plant Psoralea corylifolia L. (bu-gu-zhi in Chinese) have been widely used for treating asthma, diarrhea, waist and knee psychroalgia (Luo et al, 2014), osteoporosis (Huang et al, 2014;Li et al, 2014), kidney insufficiency, enuresis, vitiligo and alopecia areata (Pharmacopeia Committee of P.R. China, 2010).…”

Section: Introductionmentioning

confidence: 99%

Structural elucidation ofin vitrometabolites of bavachinin in rat liver microsomes by LC-ESI-MSⁿand chemical synthesis

Fan

et al. 2015

Xenobiotica

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1. Bavachinin isolated from Psoralea corylifolia has various activities, such as antimicrobial, antiallergic, antitumor and so on. Our previous study showed that natural bavachinin exhibits peroxisome proliferator-activated receptor γ-agonist activity. 2. In vitro studies on bavachinin metabolism were conducted using rat liver microsomes incubated at 37 °C for 60 min. 3. Structures of eight metabolites of the incubation mixtures were cautiously characterized using electrospray tandem mass spectra and three synthetic compounds. The results indicated that eight metabolites of bavachinin were biotransformed mainly through oxidation. 4. The metabolic pathways of bavachinin were elucidated in vitro. These results contribute to the understanding of bavachinin's in vivo metabolism.

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Osteoblasts proliferation and differentiation stimulating activities of the main components of Fructus Psoraleae corylifoliae

Cited by 99 publications

References 27 publications

Chinese single herbs and active ingredients for postmenopausal osteoporosis: From preclinical evidence to action mechanism

Chinese single herbs and active ingredients for postmenopausal osteoporosis: From preclinical evidence to action mechanism

Psoralen stimulates osteoblast proliferation through the activation of nuclear factor-κB-mitogen-activated protein kinase signaling

Structural elucidation ofin vitrometabolites of bavachinin in rat liver microsomes by LC-ESI-MSⁿand chemical synthesis

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Osteoblasts proliferation and differentiation stimulating activities of the main components of Fructus Psoraleae corylifoliae

Cited by 99 publications

References 27 publications

Chinese single herbs and active ingredients for postmenopausal osteoporosis: From preclinical evidence to action mechanism

Chinese single herbs and active ingredients for postmenopausal osteoporosis: From preclinical evidence to action mechanism

Psoralen stimulates osteoblast proliferation through the activation of nuclear factor-κB-mitogen-activated protein kinase signaling

Structural elucidation ofin vitrometabolites of bavachinin in rat liver microsomes by LC-ESI-MSnand chemical synthesis

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Structural elucidation ofin vitrometabolites of bavachinin in rat liver microsomes by LC-ESI-MSⁿand chemical synthesis