Osteocalcin biomarker level evaluation on fracture healing with bone defect after stromal vascular fraction application in murine model

Dradjat, Respati Suryanto; Sananta, Panji; Rosandi, Rizqi Daniar; Siahaan, Lasa Dhakka

doi:10.1016/j.amsu.2021.103020

Cited by 8 publications

(5 citation statements)

References 21 publications

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“…It can be inferred that the mineral deposition by osteoblast-like MG-63 cells was enhanced on 3D-printed porous implants compared to that on NP. The mineralization by MG-63 cells helps in the formation of calcium nodules and a mineralized matrix, which is a crucial step in bone regeneration . The present in vitro cell culture results showed that the porous-channel Ti6Al4 V dental implants fabricated by DMLS exhibited favorable cytocompatibility; however, in vivo experiments are needed to validate their potential for clinical translation.…”

Section: Discussionmentioning

confidence: 77%

3D Printing of Ti-6Al-4V-Based Porous-Channel Dental Implants: Computational, Biomechanical, and Cytocompatibility Analyses

Chakraborty,

Das,

Datta

et al. 2023

ACS Appl. Bio Mater.

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Loosening of dental implants due to resorption of the surrounding bone is one of the challenging clinical complications in prosthetic dentistry. Generally, stiffness mismatch between an implant and its surrounding bone is one of the major factors. In order to prevent such clinical consequences, it is essential to develop implants with customized stiffness. The present study investigates the computational and experimental biomechanical responses together with cytocompatibility studies of threedimensional (3D)-printed Ti-6Al-4V-based porous dental implants with varied stiffness properties. Methods: Additive manufacturing (direct metal laser sintering, DMLS) was utilized to create Ti-6Al-4V implants having distinct porosities and pore sizes (650 and 1000 μm), along with a nonporous (solid) implant. To validate the compression testing of the constructed implants and to probe their biomechanical response, finite element models were employed. The cytocompatibility of the implants was assessed using MG-63 cells, in vitro. Results: Both X-ray microcomputed tomography (μ-CT) and scanning electron microscopy (SEM) studies illustrated the ability of DMLS to produce implants with the designed porosities. Biomechanical analysis results revealed that the porous implants had less stiffness and were suitable for providing the appropriate peri-implant bone strain. Although all of the manufactured implants demonstrated cell adhesion and proliferation, the porous implants in particular supported better bone cell growth and extracellular matrix deposition. Conclusions: 3D-printed porous implants showed tunable stiffness properties with clinical translational potential.

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Section: Discussionmentioning

confidence: 77%

3D Printing of Ti-6Al-4V-Based Porous-Channel Dental Implants: Computational, Biomechanical, and Cytocompatibility Analyses

Chakraborty,

Das,

Datta

et al. 2023

ACS Appl. Bio Mater.

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“…The OCN protein was a product of differentiated osteoblasts in the late stage of osteogenic differentiation. 52,53 As for the secretion of OCN protein (Figure 5d−f), diluted MBG extracts except for 1/32-MBG extracts hardly improved the OCN protein secretion at 3rd and 7th days. In contrast, the diluted 1V-MBG extract significantly promoted the OCN secretion at 3, 7, and 14 days in contrast with control groups (P < 0.01).…”

Section: T H Imentioning

confidence: 88%

Vanadium-Doped Mesoporous Bioactive Glass Promotes Osteogenic Differentiation of rBMSCs via the WNT/β-Catenin Signaling Pathway

Cong,

Zhang,

et al. 2023

ACS Appl. Bio Mater.

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Pentavalent vanadium [V(V)] has been studied as bioactive ions to improve the bone defect repair; however, its osteogenic promotion mechanism is still not fully understood so far. In this study, a V-doped mesoporous bioactive glass (V-MBG) was prepared, and its effects on osteogenic differentiation of rat bone marrow mesenchymal stem cells (rBMSCs) and potential signaling pathways were investigated. The physicochemical characterization revealed that the incorporation of V slightly reduced the specific surface area and increased the mesoporous pore size, and the abundant mesopores of V-MBG were beneficial to the sustained dissolution of V(V) ions as well as calcium, silicon, and phosphorus ions. Cell proliferation results indicated that the high dilution ratio (>16) V-MBG extract markedly promoted the proliferation of rBMSCs compared with the control group and the same dilution ratio MBG extract. Compared with the same dilution ratio MBG extract, diluted V-MBG extracts markedly promoted the secretion of alkaline phosphatase (ALP) and osteocalcin (OCN) protein at day 7 but insignificantly stimulated the runt-related transcription factor 2 (RUNX2) and vascular endothelial growth factor (VEGF) protein synthesis. In depth, the diluted V-MBG extracts remarkably up-regulated the expression of WNT/β-catenin pathway direct target genes, including WNT3a, β-catenin, and AXIN2 genes in contrast to the same dilution ratio MBG extracts, suggesting that the released V(V) ions might promote osteogenic differentiation of rBMSCs via the WNT/β-catenin signaling pathway.

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“…The reviewed studies collectively demonstrate the potential applications of SVF as stimulator in the bone fracture healing process (Figure 3). The purification process ensures that unwanted components or non-functional elements are removed, leaving behind a highly enriched fraction that can be safely and effectively used for regenerative purposes [29,51,52]. The purification and analysis of SVF requires a thorough assessment of its molecular and cellular components.…”

Section: Discussionmentioning

confidence: 99%

“…The findings of Kamenaga et al [30] illuminated the potential of both freshly isolated and cryopreserved SVF cells to enhance bone healing in non-healing fracture models, thereby broadening the scope and accessibility of SVF utilization in clinical settings. Dradjat et al [29] also reported higher osteocalcin biomarker expressions in groups treated with SVF, indicating its promising role in bone metabolism and turnover.…”

Section: Steps Of Svfmentioning

confidence: 94%

Comparative Analysis of Stromal Vascular Fraction and Alternative Mechanisms in Bone Fracture Stimulation to Bridge the Gap between Nature and Technological Advancement: A Systematic Review

Goncharov,

Koval,

Nikolaevich Bezuglov

et al. 2024

Biomedicines

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Background: Various stimulation methods, including electrical, ultrasound, mechanical, and biological interventions, are explored, each leveraging intricate cellular and molecular dynamics to expedite healing. The advent of stromal vascular fraction (SVF) marks a significant stride, offering multifarious benefits in bone healing, from enhanced bone formation to optimal vascular integration, drawing a harmonious balance between innate mechanisms and scientific advancements. Methods: This systematic review was conducted focusing on literature from 2016 to 2023 and encompassing various bone healing stimulation mechanisms like SVF, electrical, ultrasound, and mechanical stimulation. The extracted data underwent meticulous synthesis and analysis, emphasizing comparative evaluations of mechanisms, applications, and outcomes of each intervention. Results: The reviewed studies reveal the potential of SVF in bone fracture healing, with its regenerative and anti-inflammatory effects. The purification of SVF is crucial for safe therapeutic use. Characterization involves flow cytometry and microscopy. Studies show SVF’s efficacy in bone regeneration, versatility in various contexts, and potential for clinical use. SVF appears superior to electrical, ultrasound, and mechanical stimulation, with low complications. Conclusions: This review compares bone healing methods, including SVF. It provides valuable insights into SVF’s potential for bone regeneration. However, due to limited human studies and potential bias, cautious interpretation is necessary. Further research is essential to validate these findings and determine the optimal SVF applications in bone healing.

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Osteocalcin biomarker level evaluation on fracture healing with bone defect after stromal vascular fraction application in murine model

Cited by 8 publications

References 21 publications

3D Printing of Ti-6Al-4V-Based Porous-Channel Dental Implants: Computational, Biomechanical, and Cytocompatibility Analyses

3D Printing of Ti-6Al-4V-Based Porous-Channel Dental Implants: Computational, Biomechanical, and Cytocompatibility Analyses

Vanadium-Doped Mesoporous Bioactive Glass Promotes Osteogenic Differentiation of rBMSCs via the WNT/β-Catenin Signaling Pathway

Comparative Analysis of Stromal Vascular Fraction and Alternative Mechanisms in Bone Fracture Stimulation to Bridge the Gap between Nature and Technological Advancement: A Systematic Review

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