Osteoclast Activity: Bone Resorption, Polarization, Acidification, Proton Pumps, and Chloride Channels

Li, Yiping; Wu, Mengrui; Chen, Wei

doi:10.1016/b978-0-12-801238-3.11165-1

Encyclopedia of Bone Biology 2020

DOI: 10.1016/b978-0-12-801238-3.11165-1

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Osteoclast Activity: Bone Resorption, Polarization, Acidification, Proton Pumps, and Chloride Channels

Yiping Li

Mengrui Wu

Wei Chen

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2024

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Cited by 1 publication

References 133 publications

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Multiomics coupled with vibrational spectroscopy identify early mechanisms of experimental aortic valve stenosis

Anousakis-Vlachochristou,

Mavroidis,

Miliotis

et al. 2024

Preprint

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Calcific aortic valve stenosis (CAVS), characterized by calcium deposition in the aortic valve in a multiannual process, is associated with high mortality and morbidity. To understand phenomena at its early stages, reliable animal models are needed. Here, we used a critically revised high-fat vitamin D2 diet rabbit model to unveil the earliest in vivo-derived mechanisms linked to CAVS progression. We modeled the inflammation-calcification temporal pattern seen in human disease and investigated molecular changes before inflammation. Coupling comprehensive multiomics and vibrational spectroscopy revealed that among the many procedures involved, mechanotransduction, peroxisome activation, DNA damage-response, autophagy, phospholipid signaling, native ECM proteins upregulation, protein cross-linking and self-folding, are the most relevant driving mechanisms. Activation of Complement 3 receptor, Immunoglobulin J and TLR6 were the earliest signs of inflammation. Among several identified key genes were AXIN2, FOS, and JUNB. Among 10 identified miRNAs, miR-21-5p and miR-204-5p dominated fundamental cellular processes, phenotypic transition, inflammatory modulation, and were validated in human samples. The enzymatic biomineralization process mediated by TNAP was complemented by V-type proton ATPase overexpression, and the substitution of Mg-pyrophosphate with Ca-pyrophosphate. These data extend our understanding on CAVS progression, facilitate the refinement of pathophysiological hypotheses and provide a basis for novel pharmaceutical therapy investigations.

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Multiomics coupled with vibrational spectroscopy identify early mechanisms of experimental aortic valve stenosis

Anousakis-Vlachochristou,

Mavroidis,

Miliotis

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

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Osteoclast Activity: Bone Resorption, Polarization, Acidification, Proton Pumps, and Chloride Channels

Cited by 1 publication

References 133 publications

Multiomics coupled with vibrational spectroscopy identify early mechanisms of experimental aortic valve stenosis

Multiomics coupled with vibrational spectroscopy identify early mechanisms of experimental aortic valve stenosis

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