Osteogenesis versus chondrogenesis by BMP-2 and BMP-7 in adipose stem cells

Knippenberg, Marlene; Helder, Marco N.; Zandieh-Doulabi, Behrouz; Wuisman, P.; Klein‐Nulend, Jenneke

doi:10.1016/j.bbrc.2006.02.052

Cited by 231 publications

(181 citation statements)

References 46 publications

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“…The procedures for collection and isolation of cells from adipose tissue were made from research by Knippenber et al 25 . These authors have proposed techniques to harvest and isolation of fat cells in the goat model.…”

Section: Discussionmentioning

confidence: 99%

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Protocols for obtainment and isolation of two mesenchymal stem cell sources in sheep

Fadel

Viana²,

Feitosa

et al. 2011

Acta Cir. Bras.

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Purpose:To evaluate different protocols to isolate stem cells from ovine umbilical cord blood and adipose tissue. Methods: There were used 5 samples of umbilical blood and 5 samples of perirenal adipose tissue from 10 female sheep. All the samples were obtained through surgery, to harvest aseptic samples. There were used 3 protocols for obtainment and culture of umbilical cord blood stem cells and 4 protocols for ovine adipose tissue stem cells. Results: It was possible to observe only one successful protocol for the obtainment of umbilical cord blood stem cells. When analyzing the techniques used to obtain adipose tissue stem cells, only one of the methods was effective as well. Through colony forming unit assay, there were obtained 58 colonies of cells after seven days in culture. Flow citometry tests revealed the cells were positive to CD44 and exhibited negative reaction to CD38, CD45, CD41/61. These cells showed a growth curve with very well defined phases LOG, LAG and PLATEAU. This phases are typically seem in mesenchymal stem cells growth curves. Conclusions: The isolation and culture of mesenchymal stem cells from ovine umbilical cord blood are complex and request more detailed assays. Stem cells from fat tissue sheep showed mesenchymal characteristics, according to their cell growth curve, ability to origin colonies of fibroblastoid cells and positive reactivity with the antibody CD44 by flow citometry. Key words: Stem Cells. Umbilical Cord. Adipose Tissue. Sheep. RESUMO Objetivo:Testar diferentes protocolos para o isolamento de células tronco a partir de sangue de cordão umbilical e tecido adiposo de ovinos. Métodos: Foram utilizadas cinco amostras de sangue de cordão umbilical e cinco amostras de tecido adiposo perirrenal de 10 fêmeas de ovelha. A coleta das amostras foi realizada através de procedimento cirúrgico para coleta do material de forma mais asséptica possível. Foram realizados três protocolos de isolamento e cultivo das células-tronco do cordão umbilical e quatro protocolos para o isolamento e cultivo das células-tronco de gordura de ovinos Resultados: Somente um dos protocolos utilizados para o isolamento das células-tronco de cordão umbilical foi efetivo. Dos quatro protocolos utilizados para isolamento das células-tronco de gordura, da mesma forma, apenas um obteve sucesso. Foi realizado o ensaio de unidades formadoras de colônias destas células, sendo contadas 58 colônias ao final de sete dias. Na citometria de fluxo essas células mostraram-se positivas para CD44 e negativas para CD38, CD45, CD41/61. Estas células apresentaram curva de crescimento com fases de LOG, LAG e PLATEAU bem definidas, características das curvas de crescimento das células-tronco de origem mesenquimal. Conclusões: O isolamento e cultivo das células-tronco mesenquimais do cordão umbilical de ovinos é de difícil realização, exigindo maiores ensaios e estudos profundos. Células tronco do tecido adiposo de ovelhas demonstraram características mesenquimais, de acordo com a curva de crescimento, habilidade de ...

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Section: Discussionmentioning

confidence: 99%

“…The procedure of collecting cells from the perirenal region was reported by Knippenberg et al 25 . His execution did not represent very complexity and there were no post-operative complications.…”

Section: Discussionmentioning

confidence: 99%

Protocols for obtainment and isolation of two mesenchymal stem cell sources in sheep

Fadel

Viana²,

Feitosa

et al. 2011

Acta Cir. Bras.

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“…In vitro studies have identified biochemical cues (Knippenberg et al 2006;Pittenger et al 1999), mechanical signals such as hydrostatic pressure and tissue deformation (Haudenschild et al 2009;Takahashi et al 1998), as well as substrate stiffness (Engler et al 2006;Park et al 2011) and oxygen availability (Holzwarth et al 2010;Fehrer et al 2007;Yun et al 2002;Kanichai et al 2008;Meyer et al 2010;Hirao et al 2006) as regulators of MSC differentiation. Identifying relationships between such stimuli and temporal and spatial patterns of tissue differentiation using in vivo experiment alone is complex, expensive and often impractical.…”

Section: Discussionmentioning

confidence: 99%

Substrate stiffness and oxygen availability as regulators of mesenchymal stem cell differentiation within a mechanically loaded bone chamber

Burke

Khayyeri

Kelly

2014

Biomech Model Mechanobiol

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Mechanical stimuli such as tissue deformation and fluid flow are often implicated as regulators of mesenchymal stem cell (MSC) differentiation during regenerative events in vivo. However, in vitro studies have identified several other physical and biochemical environmental cues, such as substrate stiffness and oxygen availability, as key regulators of stem cell fate. Hypotheses for how MSC differentiation is regulated in vivo can be either corroborated or rejected based on the ability of in silico models to accurately predict spatial and temporal patterns of tissue differentiation observed experimentally. The goal of this study was to employ a previously developed computational framework to test the hypothesis that substrate stiffness and oxygen availability regulate stem cell differentiation during tissue regeneration within an implanted bone chamber. To enable a prediction of the oxygen levels within the bone chamber, a lattice model of angiogenesis was implemented where blood vessel progression was dependent on the local mechanical environment. The model successfully predicted key aspects of MSC differentiation, including the correct spatial development of bone, marrow and fibrous tissue within the unloaded bone chamber. The model also successfully predicted chondrogenesis within the chamber upon the application of mechanical loading. This study provides further support for the hypothesis that substrate stiffness and oxygen availability regulate stem cell differentiation in vivo. These simulations also highlight the indirect role that mechanics may play in regulating MSC fate by inhibiting blood vessel progression and hence disrupting oxygen availability within regenerating tissues.

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“…Their potential strongly depends on induction by growth factors, type of scaffold and age of the patient. Growth factors from the TGFβ superfamily, such as BMPs and GDF, have demonstrated effective induction potential, especially on BMSC and ASC (Barry et al, 2001;Feng et al, 2008;Hennig et al, 2007;Hildner et al, 2010a;Knippenberg et al, 2006). However, it may take decades from the discovery of a growth factor to its clinical approval, as was the case for BMP-2 and BMP-7 in bone regeneration.…”

Section: Discussionmentioning

confidence: 99%

“…However, the proteins responsible for the induction remained unknown until the late 1980s, when BMP-2, and BMP-4 were isolated and cloned (Luyten et al, 1989;Wozney et al, 1988;Wozney, 1992). Knippenberg et al (2006) investigated the ability of BMP-2 and BMP-7 to induce differentiation and found that a short treatment for only 15 min with BMP-2 is capable of directing ASC towards the osteogenic lineage, while BMP-7 stimulates ASC to differentiate towards a chondrogenic phenotype. The addition of 500 ng/ml BMP-6 to the differentiation medium increased aggrecan gene expression by 200-fold and collagen II gene expression by 38-fold compared to the untreated control (Estes et al, 2006a).…”

Section: The Impact Of Growth Factorsmentioning

confidence: 99%

State of the art and future perspectives of articular cartilage regeneration: a focus on adipose-derived stem cells and platelet-derived products

Hildner

Albrecht

Gabriel

et al. 2011

J Tissue Eng Regen Med

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Trauma, malposition and age-related degeneration of articular cartilage often result in severe lesions that do not heal spontaneously. Many efforts over the last centuries have been undertaken to support cartilage healing, with approaches ranging from symptomatic treatment to structural cartilage regeneration. Microfracture and matrix-associated autologous chondrocyte transplantation (MACT) can be regarded as one of the most effective techniques available today to treat traumatic cartilage defects. Research is focused on the development of new biomaterials, which are intended to provide optimized physical and biochemical conditions for cell proliferation and cartilage synthesis. New attempts have also been undertaken to replace chondrocytes with cells that are more easily available and cause less donor site morbidity, e.g. adipose derived stem cells (ASC). The number of in vitro studies on adult stem cells has rapidly increased during the last decade, indicating that many variables have yet to be optimized to direct stem cells towards the desired lineage. The present review gives an overview of the difficulties of cartilage repair and current cartilage repair techniques. Moreover, it reviews new fields of cartilage tissue engineering, including stem cells, co-cultures and platelet-rich plasma (PRP).

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Osteogenesis versus chondrogenesis by BMP-2 and BMP-7 in adipose stem cells

Cited by 231 publications

References 46 publications

Protocols for obtainment and isolation of two mesenchymal stem cell sources in sheep

Protocols for obtainment and isolation of two mesenchymal stem cell sources in sheep

Substrate stiffness and oxygen availability as regulators of mesenchymal stem cell differentiation within a mechanically loaded bone chamber

State of the art and future perspectives of articular cartilage regeneration: a focus on adipose-derived stem cells and platelet-derived products

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