Osteogenic potency of human bone marrow mesenchymal stem cells from femoral atrophic non-union fracture site

Dilogo, Ismail Hadisoebroto; Phedy, Phedy; Kholinne, Erica; Djaja, Yoshi Pratama; Kusnadi, Yuyus; Sandhow, Lakshmi

doi:10.5799/ahinjs.01.2014.02.0382

Cited by 1 publication

(2 citation statements)

References 16 publications

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“…We detected significantly lower osteogenic marker levels in uncultured NU BM- CD271 high CD45 low cells despite the osteogenic ability shown for culture-expanded cells in this study and previous work 41 . In NU-serum supplemented cultures, all osteogenic genes, except ALPL showed comparable levels versus U-cultures.…”

Section: Discussioncontrasting

confidence: 95%

“…Noteworthy, lower numbers of BM-derived colony-forming cells were reported for atrophic non-union than healed fractures patients 39 ; however, no difference was found in BM-MSC numbers expressing osteoprogenitor marker, CD271 high in another study 40 . Other studies have reported a similar osteogenic function of MSCs resident within non-union tissues relative to donor-matched BM-MSCs but without comparison to union-MSCs 36,37,41,42 . Furthermore, the immunoregulatory potential of non-union BM-MSCs is mostly unknown despite being an important part of the bone healing process.…”

Section: Introductionmentioning

confidence: 85%

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Defective Proliferation and Osteogenic Potential with Altered Immunoregulatory phenotype of Native Bone marrow-Multipotential Stromal Cells in Atrophic Fracture Non-Union

El-Jawhari

Kleftouris

El-Sherbiny

et al. 2019

Sci Rep

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Bone marrow-Multipotential stromal cells (BM-MSCs) are increasingly used to treat complicated fracture healing e.g., non-union. Though, the quality of these autologous cells is not well characterized. We aimed to evaluate bone healing-related capacities of non-union BM-MSCs. Iliac crest-BM was aspirated from long-bone fracture patients with normal healing (U) or non-united (NU). Uncultured (native) CD271highCD45low cells or passage-zero cultured BM-MSCs were analyzed for gene expression levels, and functional assays were conducted using culture-expanded BM-MSCs. Blood samples were analyzed for serum cytokine levels. Uncultured NU-CD271highCD45low cells significantly expressed fewer transcripts of growth factor receptors, EGFR, FGFR1, and FGRF2 than U cells. Significant fewer transcripts of alkaline phosphatase (ALPL), osteocalcin (BGLAP), osteonectin (SPARC) and osteopontin (SPP1) were detected in NU-CD271highCD45low cells. Additionally, immunoregulation-related markers were differentially expressed between NU- and U-CD271highCD45low cells. Interestingly, passage-zero NU BM-MSCs showed low expression of immunosuppressive mediators. However, culture-expanded NU and U BM-MSCs exhibited comparable proliferation, osteogenesis, and immunosuppression. Serum cytokine levels were found similar for NU and U groups. Collectively, native NU-BM-MSCs seemed to have low proliferative and osteogenic capacities; therefore, enhancing their quality should be considered for regenerative therapies. Further research on distorted immunoregulatory molecules expression in BM-MSCs could potentially benefit the prediction of complicated fracture healing.

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Section: Discussioncontrasting

confidence: 95%