Osteoinduction in human fat-derived stem cells by recombinant human bone morphogenetic protein-2 produced in Escherichia coli

Bessa, Paulo C.; Pedro, A. J.; Klösch, Burkhard; Nobre, Alexandra; Griensven, Martijn van; Reis, Rui L.; Casal, Margarida

doi:10.1007/s10529-007-9501-8

Cited by 25 publications

(25 citation statements)

References 30 publications

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“…In this study, the system of gelatin hydrogels for combined release of SDF-1 and BMP-2 synergistically enhanced bone regeneration. SDF-1 has an inherent ability to enhance the recruitment of hematopoietic cells [14e17] and BMP-2 has a potential to promote the osteogenic differentiation of bone progenitor cells [20,21] and to recruit the cells [8,9]. If the release of two protein factors works well, it is theoretically possible that the recruitment of hematopoietic and bone progenitor cells will be promoted.…”

Section: Discussionmentioning

confidence: 99%

“…Among osteogenic growth factors, bone morphogenetic protein-2 (BMP-2) is well-known as a strong inducer of bone tissue formation by enhancing the recruitment of osteoblast progenitor cells [8], angiogenesis [19], and promoting the osteogenic differentiation of mesenchymal stem cells [20,21]. The BMP-2 treatment induced complete bone regeneration at a bone defect [22] and formed bone tissues ectopically for the animal models [8,9].…”

Section: Introductionmentioning

confidence: 99%

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Synergistic effects of the dual release of stromal cell-derived factor-1 and bone morphogenetic protein-2 from hydrogels on bone regeneration

et al. 2011

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The objective of this study is to evaluate the activity of gelatin hydrogels incorporating combined stromal cell-derived factor-1 (SDF-1) and bone morphogenetic protein-2 (BMP-2) on the in vivo bone regeneration at an ulna critical-sized defect and subcutaneous site of rats, and compared with that of those incorporating either SDF-1 or BMP-2. The similar release profile of SDF-1 and BMP-2 from the hydrogels was observed with or without the combination of BMP-2 and SDF-1, respectively. An enhanced bone regeneration by the hydrogels incorporating combined SDF-1 and BMP-2 was observed. In addition, the implantation of hydrogels incorporating combined SDF-1 and BMP-2 enhanced the expression level of CXC chemokine cell-surface receptor-4 (Cxcr4), Runt-related factor-2 (Runx2), and Osteocalcin genes. The experiments with green fluorescent protein (GFP)-positive Chimeric mice revealed that the recruitment of bone marrow-derived cells was promoted and a vascular-like structure together with strong accumulation of CD31- and CD34-positive cells was observed at the site of hydrogels incorporating combined SDF-1 and BMP-2 implanted. In addition, a large fraction of CD29- and CD44-positive non-hematopoietic cells was detected. It is concluded that the combined release of SDF-1 and BMP-2 enhanced the recruitment of osteogenic cells and angiogenesis, resulting in the synergistic effect on bone regeneration.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synergistic effects of the dual release of stromal cell-derived factor-1 and bone morphogenetic protein-2 from hydrogels on bone regeneration

et al. 2011

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“…In early studies, the poor efficacy of ErhBMP-2 was thought to be due to the inefficient refolding process of the BMP-2 protein after expression in E. coli [15]. In recent years, successful methods for restoring the biologic activity after rhBMP-2 expression in E. coli have made it possible to produce fully functional BMP-2 from E. coli [10,24]. In addition, it was shown that post-translational glycosylation was not critical for a fully functional ErhBMP-2, because the basic glycosylated N-terminal domains of rhBMP-2 were not obligatory for receptor activation [25].…”

Section: Discussionmentioning

confidence: 99%

The induction of bone formation in rat calvarial defects and subcutaneous tissues by recombinant human BMP-2, produced in Escherichia coli

Lee

Kim

Choi³

et al. 2010

Biomaterials

101

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“…Fat-derived stem cells have been used in the repair of bone (Bessa et al, 2008;Betz et al, 2010), and are regarded as potentially suitable for the repair of soft-tissue defects (Vallée et al, 2009). Applications to the repair of airway defects are, however, so far few.…”

Section: Gm Roomansmentioning

confidence: 99%

Tissue engineering and the use of stem/progenitor cells for airway epithelium repair

Roomans

2010

eCM

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Stem/progenitor cells can be used to repair defects in the airway wall, resulting from e.g., tumors, trauma, tissue reactions following long-time intubations, or diseases that are associated with epithelial damage. Several potential sources of cells for airway epithelium have been identified. These can be divided into two groups. The first group consists of endogenous progenitor cells present in the respiratory tract. This group can be subdivided according to location into (a) a ductal cell type in the submucosal glands of the proximal trachea, (b) basal cells in the intercartilaginous zones of the lower trachea and bronchi, (c) variant Clara cells (Clara v -cells) in the bronchioles and (d) at the junctions between the bronchioles and the alveolar ducts, and (e) alveolar type II cells. This classification of progenitor cell niches is, however, controversial. The second group consists of exogenous stem cells derived from other tissues in the body. This second group can be subdivided into: (a) embryonic stem (ES) cells, induced pluripotent stem (iPS) cells, or amniotic fluid stem cells, (b) side-population cells from bone marrow or epithelial stem cells present in bone marrow or circulation and (c) fat-derived mesenchymal cells. Airway epithelial cells can be co-cultured in a system that includes a basal lamina equivalent, extracellular factors from mesenchymal fibroblasts, and in an air-liquid interface system. Recently, spheroid-based culture systems have been developed. Several clinical applications have been suggested: cystic fibrosis, acute respiratory distress syndrome, chronic obstructive lung disease, pulmonary fibrosis, pulmonary edema, and pulmonary hypertension. Clinical applications so far are few, but include subglottic stenosis, tracheomalacia, bronchiomalacia, and emphysema.

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Osteoinduction in human fat-derived stem cells by recombinant human bone morphogenetic protein-2 produced in Escherichia coli

Cited by 25 publications

References 30 publications

Synergistic effects of the dual release of stromal cell-derived factor-1 and bone morphogenetic protein-2 from hydrogels on bone regeneration

Synergistic effects of the dual release of stromal cell-derived factor-1 and bone morphogenetic protein-2 from hydrogels on bone regeneration

The induction of bone formation in rat calvarial defects and subcutaneous tissues by recombinant human BMP-2, produced in Escherichia coli

Tissue engineering and the use of stem/progenitor cells for airway epithelium repair

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