Osteoinduction Using a Prefabricated Scaffold in the Gluteus Muscle of Wistar Rats

Abstract

Introduction: Bone grafts are very significant for the reconstruction of the trauma and the deformations created thereafter. Various bone forming and promoting agents are widely studied. Hence, in this study, we aim to evaluate the osteoinduction effect of scaffold impregnated with Simvastatin in wistor rats in an observational study. Material and Methods: We implanted 18 Wistar rats to be equally divided as controls and test groups. Polycaprolactone coated with 20 mg concentration of Simavastatin under sterile condition was used as test and hydroxy appetite as control. Then, we observed the histopathological Heamatoxilin and Eosin and ABH, sections at 4, 12, and 26 weeks. We noted the calcium deposition and bone formation. Results: We observed a significant variation among groups at 4, 12, and 26 weeks in the calcium deposition. While for the bone formation no significant variation were noted among groups at 4 and 12 weeks, however, there was a significant difference noted at 26 weeks. At the end of 12 weeks, mild fibroblast proliferation was seen in the surrounding area. After 26 weeks, ABH sections showed focal area of fibroblast proliferation with mild mononuclear infiltration was noticed; but implant could not be located. Conclusion: We conclude that Simavastatin showed an anabolic effect on bone healing.