Osteoinductive activity of biphasic calcium phosphate with different rhBMP-2 doses in rats

Jang, Jyongsik; Yun, Jin-Mun; Lee, Kwang; Jang, Ju Woong; Jung, Ui Won; Kim, Chang Sung; Choi, Seong Ho; Cho, Kyoo-Sung

doi:10.1016/j.tripleo.2011.04.013

Cited by 70 publications

(59 citation statements)

References 33 publications

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“…Jang et al assessed BCP loaded with different doses of rhBMP-2 in critical-sized calvarial defects in rats. Percentages of new bone were significantly greater in the rhBMP-2-treated groups than in the pure BCP group [18]. These results indicated BCP was a good carrier and scaffold for rhBMP-2.…”

Section: Discussionmentioning

confidence: 73%

“…BCP could promote adhesion, proliferation and differentiation of osteocytes [16,17]. However, pure BCP has limited bone regeneration capacity; the bioactivity could be enhanced by growth factors such as recombinant bone morphogenetic protein-2 (rhBMP-2) [18]. In another way, BCP is a good carrier for rhBMP-2 owing to excellent biocompatibility, biomechanics, high porosity and pore structure [19,20].…”

Section: Introductionmentioning

confidence: 99%

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Lumbar interbody fusion with porous biphasic calcium phosphate enhanced by recombinant bone morphogenetic protein-2/silk fibroin sustained-released microsphere: an experimental study on sheep model

Chen

Liu

et al. 2015

J Mater Sci: Mater Med

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Biphasic calcium phosphate (BCP) has been investigated extensively as a bone substitute nowadays. However, the bone formation capacity of BCP is limited owing to lack of osteoinduction. Silk fibroin (SF) has a structure similar to type I collagen, and could be developed to a microsphere for the sustained-release of rhBMP-2. In our previous report, bioactivity of BCP could be enhanced by rhBMP-2/SF microsphere (containing 0.5 µg rhBMP-2) in vitro. However, the bone regeneration performance of the composite in vivo was not investigated. Thus, the purpose of this study was to evaluate the efficacy of BCP/rhBMP-2/SF in a sheep lumbar fusion model. A BCP and rhBMP-2/SF microsphere was developed, and then was integrated into a BCP/rhBMP-2/SF composite. BCP, BCP/rhBMP-2 and BCP/rhBMP-2/SF were implanted randomly into the disc spaces of 30 sheep at the levels of L1/2, L3/4 and L5/6. After sacrificed, the fusion segments were evaluated by manual palpation, CT scan, biomechanical testing and histology at 3 and 6 months, respectively. The composite demonstrated a burst-release of rhBMP-2 (39.1 ± 2.8 %) on the initial 4 days and a sustained-release (accumulative 81.3 ± 4.9 %) for more than 28 days. The fusion rates, semi-quantitative CT scores, fusion stiffness in bending in all directions and histologic scores of BCP/rhBMP-2/SF were significantly greater than BCP and BCP/rhBMP-2 at each time point, respectively (P < 0.05). These findings indicate that the SF microspheres containing a very low dose of rhBMP-2 improve fusion in sheep using BCP constructs.

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Section: Discussionmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Lumbar interbody fusion with porous biphasic calcium phosphate enhanced by recombinant bone morphogenetic protein-2/silk fibroin sustained-released microsphere: an experimental study on sheep model

Chen

Liu

et al. 2015

J Mater Sci: Mater Med

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“…To clarify, BCP is a combination of HA and ␤-TCP and has been widely applied in clinical and research fields because of its capacity for osteoconductivity and osteoinductivity. 37,38 The results of the study showed that the residual biomaterials occupied a large portion of newly formed bone area in the BCP sites, and all CCP sites showed no residual biomaterials. This could be due to the HA composition of BCP and the difference of particle size between BCP and CCP.…”

Section: Discussionmentioning

confidence: 94%

Guided Bone Regeneration Using Cyanoacrylate-Combined Calcium Phosphate in a Dehiscence Defect: A Histologic Study in Dogs

Lee¹,

Ko²,

Kim³

et al. 2012

Journal of Oral and Maxillofacial Surgery

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“…This would mean that, in order to assess growth factor synergy, the volume of the chambers should be enlarged to a size where vascularisation is critical for bone formation. Furthermore, the concentration of BMP-2 was based on findings in the literature (Jang et al, 2012), and was supraphysiological. Together with the confined space offered by the chamber, this may have led to maximal bone induction, hereby masking possible synergistic or additive effects of the other growth factors, that are well established for VEGF (Kanczler et al, 2010;Kempen et al, 2009) and also reported for SDF-1α (Ratanavaraporn et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Growth factor-induced osteogenesis in a novel radiolucent bone chamber

Poldervaart¹,

Stok²,

Haas³

et al. 2015

eCM

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Treatment of large bone defects is currently performed using mainly autograft or allograft bone. There are important drawbacks to bone grafting, such as limited availability, donor site morbidity in the case of autograft and inferior performance of allografts. Therefore, there is a great need for a suitable bone graft substitute. In order to evaluate efficiently newly developed biomaterials and factors intended for orthopaedic surgery, the bone chamber is a very suitable model. To allow longitudinal investigation of bone growth with μCT, a new bone chamber made of radiolucent polyether ether ketone (PEEK) was developed and studied for its feasibility. Therefore, PEEK bone chambers were placed on rat tibiae, and filled with vehicle (Matrigel without growth factors, negative controls), with bone morphogenetic protein 2 (BMP-2, positive controls), or a mix of growth factors combining BMP-2, vascular endothelial growth factor and the chemokine stromal cell-derived factor 1α, all laden on gelatin microspheres for controlled release (combined growth factors). Growth factor presence led to a significant increase in bone formation after 8 weeks, which subsided after 12 weeks, underlining the importance of longitudinal analysis. We conclude that the PEEK-bone chamber is a suitable translational animal model to assess orthotopic bone formation in a longitudinal manner.

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Osteoinductive activity of biphasic calcium phosphate with different rhBMP-2 doses in rats

Cited by 70 publications

References 33 publications

Lumbar interbody fusion with porous biphasic calcium phosphate enhanced by recombinant bone morphogenetic protein-2/silk fibroin sustained-released microsphere: an experimental study on sheep model

Lumbar interbody fusion with porous biphasic calcium phosphate enhanced by recombinant bone morphogenetic protein-2/silk fibroin sustained-released microsphere: an experimental study on sheep model

Guided Bone Regeneration Using Cyanoacrylate-Combined Calcium Phosphate in a Dehiscence Defect: A Histologic Study in Dogs

Growth factor-induced osteogenesis in a novel radiolucent bone chamber

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