Osteomatrix as a personalized 3D tissue-specific invasion test-bed for oral carcinoma

Sukanya, V S; Giri, Pravin Shankar; Nellore, Vijaykumar; Rath, Subha Narayan

doi:10.1039/d2bm01870e

Cited by 4 publications

(4 citation statements)

References 77 publications

(81 reference statements)

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“…39 On top of hydrogel coatings, 5 × 10 4 cells were seeded in each well of a 24-well plate, and assays were performed at time intervals of 3, 7, and 14 days. 39 Macrophage Activation for Control Macrophages. THP-1 monocytes at a cell density of 4 × 10 5 were seeded in each well of a 24well plate.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…Subsequently, THP-1 cells at a density of 30,000 per well were introduced onto the coated plate. 39 At specific time intervals of day 7 and day 14, the cells were washed with 1 × PBS and incubated with FDA (1 μg/mL) for 15 min, followed by a PI (20 μg/mL) solution for 2 min. After an additional wash with 1 × PBS, images were captured by using a fluorescence microscope (EVOS M7000).…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…The THP-1 cell line acquired from NCCS, Pune, India, was cultured in RPMI-1640 (PAN-Biotech, Germany) containing 10% FBS and 1% P/S in a CO 2 incubator maintaining 5% CO 2 , 95% humidity, and temperature of 37 °C. For differentiation into classical M0, pro-inflammatory M1, and anti-inflammatory M2 macrophages, THP-1 cells were activated with 10 ng/mL PMA (phorbol 12-myristate 13-acetate, (Cayman Chemicals, USA)), 100 ng/mL LPS (lipopolysaccharide) (Cayman Chemicals, USA), and 5 ng/mL IL-4 (interleukin 4) (ProSpec-Tany, TechnoGene, Israel), respectively . On top of hydrogel coatings, 5 × 10 4 cells were seeded in each well of a 24-well plate, and assays were performed at time intervals of 3, 7, and 14 days …”

Section: Methodsmentioning

confidence: 99%

“…In a 24-well tissue culture plate, 100 μL of each hydrogel was coated and cross-linked. Subsequently, THP-1 cells at a density of 30,000 per well were introduced onto the coated plate . At specific time intervals of day 7 and day 14, the cells were washed with 1 × PBS and incubated with FDA (1 μg/mL) for 15 min, followed by a PI (20 μg/mL) solution for 2 min.…”

Section: Methodsmentioning

confidence: 99%

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Macrophage Polarization Dynamics in Biomaterials: Implications for in Vitro Wound Healing

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2024

ACS Appl. Bio Mater.

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The interaction between biomaterials and the immune system plays a pivotal role in determining the success or failure of implantable devices. Macrophages, as key orchestrators of immune responses, exhibit diverse reactions that influence tissue integration or lead to implant failure. This study focuses on unraveling the intricate relationship between macrophage phenotypes and biomaterials, specifically hydrogels, by employing THP-1 cells as a model. Through a comprehensive investigation using polysaccharide, polymer, and protein-based hydrogels, our research sheds light on how the properties of hydrogels influence macrophage polarization. Phenotypic observations, biochemical assays, surface marker expression, and gene expression profiles collectively demonstrate the differential macrophage polarization abilities of polysaccharide-, polymer-, and protein-based hydrogels. Moreover, our indirect coculture studies reveal that hydrogels fostering M2 polarization exhibit exceptional wound-healing capabilities. These findings highlight the crucial role of the hydrogel microenvironment in adjusting macrophage polarization, offering a fresh avenue for refining biomaterials to bolster advantageous immune responses and improve tissue integration. This research contributes valuable insights for designing biomaterials with tailored properties that can guide macrophage behavior, ultimately improving the overall success of implantable devices.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Macrophage Polarization Dynamics in Biomaterials: Implications for in Vitro Wound Healing

Giri,

Rath

2024

ACS Appl. Bio Mater.

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Differential osteo-specific invasion of patient-derived cancer cells in a microfluidic co-culture model

Sukanya.,

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et al. 2024

Chemical Engineering Journal

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Drug screening on digital microfluidics for cancer precision medicine

Zhai,

Liu,

et al. 2024

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Drug screening based on in-vitro primary tumor cell culture has demonstrated potential in personalized cancer diagnosis. However, the limited number of tumor cells, especially from patients with early stage cancer, has hindered the widespread application of this technique. Hence, we developed a digital microfluidic system for drug screening using primary tumor cells and established a working protocol for precision medicine. Smart control logic was developed to increase the throughput of the system and decrease its footprint to parallelly screen three drugs on a 4 × 4 cm2 chip in a device measuring 23 × 16 × 3.5 cm3. We validated this method in an MDA-MB-231 breast cancer xenograft mouse model and liver cancer specimens from patients, demonstrating tumor suppression in mice/patients treated with drugs that were screened to be effective on individual primary tumor cells. Mice treated with drugs screened on-chip as ineffective exhibited similar results to those in the control groups. The effective drug identified through on-chip screening demonstrated consistency with the absence of mutations in their related genes determined via exome sequencing of individual tumors, further validating this protocol. Therefore, this technique and system may promote advances in precision medicine for cancer treatment and, eventually, for any disease.

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Osteomatrix as a personalized 3D tissue-specific invasion test-bed for oral carcinoma

Abstract: The schematic illustration of the workflow to develop a 3D in vitro patient-specific bone invasion model for oral carcinoma.

Cited by 4 publications

References 77 publications

Macrophage Polarization Dynamics in Biomaterials: Implications for in Vitro Wound Healing

Macrophage Polarization Dynamics in Biomaterials: Implications for in Vitro Wound Healing

Differential osteo-specific invasion of patient-derived cancer cells in a microfluidic co-culture model

Drug screening on digital microfluidics for cancer precision medicine

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