Osteopontin is differentially expressed in renal cell tumors

Woźniak, Marta; Pawelak, Agnieszka; Makuch, Sebastian; Martuszewski, Adrian; Winograd, Kinga; Ziółkowski, Piotr; Agrawal, Siddarth

doi:10.1080/01478885.2019.1710041

Cited by 1 publication

(1 citation statement)

References 18 publications

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“…Furthermore, over expression of SPP1 has been found in bladder ( 15 ), prostate ( 16 ), breast ( 17 ), colon ( 18 ), and lung cancers ( 19 ). Besides, the SPP1 expression level in cancer is related to tumor stage and aggressiveness ( 20 ). Ahmed et al reported that SPP1 secreted by macrophages could combine with CD44s on the surface of cancer cells ( 11 ).…”

Section: Introductionmentioning

confidence: 99%

SPP1 mRNA determination based on molecular beacon for the recurrence prognosis of bladder cancer

Wu,

Wang,

Zhang

et al. 2023

Transl Androl Urol

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Background Bladder cancer (BC) has attracted significant attention on account of its recurrence as well as mortality. Tumor recurrence plays a significant role in cancer patients’ individual treatment. Secreted phosphoprotein 1 (SPP1) has been recognized as a potential target for treating BC and served as a useful biomarker for prognosis; it is commonly tested by immunohistochemistry (IHC). However, this conventional method has the disadvantage of being time-consuming and costly. This study aimed to develop a molecular beacon (MB) for the detection of SPP1 messenger RNA (mRNA) for the recurrence prognosis of BC. Methods An MB was constructed and applied to image SPP1 mRNA level at both molecular and cellular level. The fluorescence spectra were recorded with a fluorescence spectrophotometer. The effect of SPP1 MB toward the cell viability was performed by Cell Counting Kit-8 (CCK-8) assays. The SPP1 mRNA expression level was measured by quantitative real-time polymerase chain reaction (qRT-PCR). Cancer cells and tissues were analyzed with confocal fluorescence imaging. Correlation, sensitivity, and specificity parameters were calculated. Results It was demonstrated that both cancer cells and BC tissues expressed high signal which reflected the expression of SPP1. In addition, 42 cases were detected by MB and divided into two groups according to the fluorescence intensity. The results further suggested that highly expressed SPP1 could predict early tumor recurrence in BC. Conclusions The SPP1 MB could be applied as an appropriate approach to predict BC recurrence and patients’ prognosis.

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