Osterix plays a critical role in BMP4-induced promoter activity of connexin43

Han, Younho; Cho, Dong Hyeok; Chung, Dong Jin; Lee, Kwang Youl

doi:10.1016/j.bbrc.2016.08.007

Cited by 12 publications

(8 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar to Osx expression pattern, expression of Cx43 at day 3 culture was higher for A1-, A2 and S1/PLGA scaffolds. This is consistent with literature showing important role of Osx in Cx43 expression[44][45]. Importantly, we also observed relatively higher levels of Cx43 for cells cultured on the scaffolds without P 2 O 5 .…”

supporting

confidence: 93%

Molecular Indicators of Biomaterials Osteoinductivity - Cell Migration, BMP Production and Signalling Turns a Key

Łukowicz

Zagrajczuk

Wieczorek

et al. 2021

Preprint

View full text Add to dashboard Cite

In this work we dissect the osteoinductive properties of selected bioactive materials obtained in a 3D form, and based on PLGA matrix and 2 types of gel-derived bioactive glasses (SBGs) of SiO2-CaO system, each with and without P2O5. The study is designed to avoid any osteogenic stimuli beyond the putative osteogenic bioactive glass compound of the studied materials. Previously we found that, when used as growth surfaces (i.e. material sheets), some of these materials were capable to support osteogenesis of bone marrow stromal cells (BMSC) without the need for any additional osteogenic cell treatment. In this work we explore further the role of BMP production and signaling upon BMSC culture on selected, SBG/PLGA 3D scaffolds as well as BMSC migration toward the condition media obtained from such cell-loaded materials. Our results show that BMP signaling of osteoprogenitor cells as well as their migration rate may present important indicators of materials osteoinductivity.

show abstract

supporting

confidence: 93%

Molecular Indicators of Biomaterials Osteoinductivity - Cell Migration, BMP Production and Signalling Turns a Key

Łukowicz

Zagrajczuk

Wieczorek

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Sp7 has been shown to upregulate transcription of various osteoblast-related genes, including Fmod (fibromodulin) [ 29 ], Col1a1 [ 30 , 31 ], Col1a2 [ 32 ], Col5a1 [ 33 ], Col5a3 [ 34 ], Ibsp [ 29 , 35 ], Sost [ 13 , 36 ], Bglap [ 37 ], Zbtb16 [ 38 ], Cx43 [ 39 ], Vegf (vascular endothelial growth factor) [ 40 ], Mmp9 (matrix metalloproteinase 9) [ 41 ], Mmp13 (matrix metalloproteinase 13) [ 42 , 43 ], Zip1 [ 44 ], Ucma (upper zone of growth plate and cartilage matrix associated) [ 45 ], and Enpp1 (pyrophosphatase/phosphodiesterase 1) [ 46 ] (extensively reviewed in [ 47 ]). This series of studies demonstrated that Sp7 bound to the typical GC-box (Sp1-binding sites) or CCAAT sequences around these genes ( Figure 1 ).…”

Section: Targets Of Sp7 In Osteoblastsmentioning

confidence: 99%

Sp7 Action in the Skeleton: Its Mode of Action, Functions, and Relevance to Skeletal Diseases

Hojo

Ohba

2022

IJMS

View full text Add to dashboard Cite

Osteoblast differentiation is a tightly regulated process in which key transcription factors (TFs) and their target genes constitute gene regulatory networks (GRNs) under the control of osteogenic signaling pathways. Among these TFs, Sp7 works as an osteoblast determinant critical for osteoblast differentiation. Following the identification of Sp7 and a large number of its functional studies, recent genome-scale analyses have made a major contribution to the identification of a “non-canonical” mode of Sp7 action as well as “canonical” ones. The analyses have not only confirmed known Sp7 targets but have also uncovered its additional targets and upstream factors. In addition, biochemical analyses have demonstrated that Sp7 actions are regulated by chemical modifications and protein–protein interaction with other transcriptional regulators. Sp7 is also involved in chondrocyte differentiation and osteocyte biology as well as postnatal bone metabolism. The critical role of SP7 in the skeleton is supported by its relevance to human skeletal diseases. This review aims to overview the Sp7 actions in skeletal development and maintenance, particularly focusing on recent advances in our understanding of how Sp7 functions in the skeleton under physiological and pathological conditions.

show abstract

“…It was reported that Cx43 expression was significantly repressed by the addition of shRNA against Osx, whereas overexpression of Osx resulted in enhanced Cx43 expression. Further studies have proven that Osx can directly occupy the promoter region of Cx43 and subsequently increases Cx43 promoter activity in a dosedependent manner (Han et al, 2016a;Chen et al, 2019). Recent studies have shown that bone marrow adipocytes not only suppress osteoblasts, but also promote bone resorption by the recruitment of osteoclasts, and Osx represses adipogenesis by negatively regulating PPARγ expression and transcriptional activity (Han et al, 2016b;Li et al, 2018b).…”

Section: The Function Of Osx In Osteoclastsmentioning

confidence: 99%

“…Osx increases the promoter activity of Cx43 by directly interacting with the Cx43 promoter and subsequently upregulates the expression level of Cx43. As a result, the expression and transcriptional activity of Cx43 were considerably affected by Osx (Han et al, 2016a).…”

Section: Osx Promotes Osteogenesis Through the Regulation Of Downstream Factorsmentioning

confidence: 99%

Recent Advances of Osterix Transcription Factor in Osteoblast Differentiation and Bone Formation

Liu

Wang

et al. 2020

Front. Cell Dev. Biol.

147

View full text Add to dashboard Cite

With increasing life expectations, more and more patients suffer from fractures either induced by intensive sports or other bone-related diseases. The balance between osteoblast-mediated bone formation and osteoclast-mediated bone resorption is the basis for maintaining bone health. Osterix (Osx) has long been known to be an essential transcription factor for the osteoblast differentiation and bone mineralization. Emerging evidence suggests that Osx not only plays an important role in intramembranous bone formation, but also affects endochondral ossification by participating in the terminal cartilage differentiation. Given its essentiality in skeletal development and bone formation, Osx has become a new research hotspot in recent years. In this review, we focus on the progress of Osx’s function and its regulation in osteoblast differentiation and bone mass. And the potential role of Osx in developing new therapeutic strategies for osteolytic diseases was discussed.

show abstract

Osterix plays a critical role in BMP4-induced promoter activity of connexin43

Cited by 12 publications

References 29 publications

Molecular Indicators of Biomaterials Osteoinductivity - Cell Migration, BMP Production and Signalling Turns a Key

Molecular Indicators of Biomaterials Osteoinductivity - Cell Migration, BMP Production and Signalling Turns a Key

Sp7 Action in the Skeleton: Its Mode of Action, Functions, and Relevance to Skeletal Diseases

Recent Advances of Osterix Transcription Factor in Osteoblast Differentiation and Bone Formation

Contact Info

Product

Resources

About