Ostreopsis cf. ovata from western Mediterranean Sea: Physiological responses under different temperature and salinity conditions

Carnicer, Olga; García‐Altares, María; Andrée, Karl B.; Tartaglione, Luciana; Dell’Aversano, Carmela; Ciminiello, Patrizia; Iglesia, Pablo de la; Diogène, Jorge; Fernández-Tejedor, Margarita

doi:10.1016/j.hal.2016.06.002

Cited by 28 publications

(11 citation statements)

References 54 publications

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“…Toxin profiles did not vary significantly among cells collected during the bloom (i.e., on artificial substrates, see Supplementary data S2), among cells collected on P. pavonica according to depth or among cells attached to the three macroalgae. Similar observations have previously been made in situ (Accoroni et al, 2011;Pfannkuchen et al, 2012;Brissard et al, 2014) and in controlled experiments (Pezzolesi et al, 2012;Vanucci et al, 2012;Pezzolesi et al, 2014;Carnicer et al, 2016;Pezzolesi et al, 2016;Mendes et al, 2017).…”

Section: Toxin Profilessupporting

confidence: 88%

“…Indeed, four different toxin profiles were reported for strains isolated from the Mediterranean Sea, differing qualitatively (i.e., in the number of OVTXs) and thus in terms of analogue proportions . The most common strain contains OVTX-a as the major analogue, followed by OVTX-b (Brissard et al, 2014;Ciminiello et al, 2014;García-Altares et al, 2015;Carnicer et al, 2016;Ninčević Gladan et al, 2019). The two other profiles were restricted to only four strains out of the fifty-five that were screened including non OVTX-producing strains (Tartaglione et al, 2017).…”

Section: Different Time Windows Of Blooms Can Be Observed In the Northwestmentioning

confidence: 99%

“…Effects of growth and OVTX-modulating factors on O. cf. ovata toxin content have been largely studied in laboratory experiments (Guerrini et al, 2010;Pistocchi et al, 2011;Nascimento et al, 2012;Pezzolesi et al, 2012;Brissard et al, 2014;Carnicer et al, 2016). However, data on OVTX content from field studies (e.g., to look at how content depends on bloom phases and biotic or abiotic factors) are scarcer (Pfannkuchen et al, 2012;Accoroni et al, 2017;Ninčević Gladan et al, 2019).…”

Section: Different Time Windows Of Blooms Can Be Observed In the Northwestmentioning

confidence: 99%

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Toxin content of Ostreopsis cf. ovata depends on bloom phases, depth and macroalgal substrate in the NW Mediterranean Sea

et al. 2020

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Over the last fifteen years, blooms of the genus Ostreopsis have been reported more frequently and at higher abundances in the Mediterranean area. Ostreopsis cf. ovata is known to produce ovatoxins (OVTXs), structural analogues of palytoxin, which is one of the most potent non-polymeric toxins. However, the production of OVTXs is poorly characterized in situ. The present study focuses on toxin content and profile according to the bloom phase during summer 2017 in Villefranche-sur-Mer, France (NW Mediterranean Sea), depth (from 0.5 to 5 m) and three different macroalgal substrates of this epiphytic dinoflagellate (Padina pavonica, Dictyota spp. and Halopteris scoparia). Ovatoxin quantification of all samples was performed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The bloom started at the end of June and declined in mid-July, showing the typical seasonal pattern of the NW Mediterranean Sea area. The peak was observed on the 10 July with 1.8 × 106 cells/g FW and 1.7 × 104 cells/L for benthic and planktonic cells, respectively. Total toxin content of cells, collected using artificial substrates, increased during the exponential and stationary growth phases. After reaching a maximum concentration of 9.2 pg/cell on 18 July, toxin concentration decreased and remained stable from 25 July until the end of monitoring. A decreasing trend of the abundance and of the associated total toxin content was noted with depth. Finally, the decreasing order of maximal epiphytic concentration of O. cf. ovata was: Dictyota spp. (8.3 × 105 cells/g FW), H. scoparia (3.1 × 105 cells/g FW) and P. pavonica (1.6 × 105 cells/g FW). Interestingly, the highest OVTX quota was obtained in cells present on Halopteris scoparia, then on Dictyota spp. and Padina pavonica. This suggests that the nature of the macroalgal substrate influences both growth and toxin production of O. cf. ovata and further work will be required to understand the underlying mechanisms (e.g., competition for nutrition, pH or allelopathic interaction). However, the toxin profiles (i.e., the proportion of each ovatoxin analogue) were not affected by any of the studied parameters (bloom phase, depth, macroalgae or artificial substrates). Highlights► Ostreopsis cf. ovata bloomed from late June to August 2017 at Villefranche-sur-Mer, France, Mediterranean Sea. ► Ovatoxins contents were higher during the exponential and stationary growth phases. ► Highest abundance was on Dictyota spp. but highest toxin quota on Halopteris scoparia. ► Ovatoxin profile was not affected by the bloom phase, the depth or the macroalgae.

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Section: Toxin Profilessupporting

confidence: 88%

Section: Different Time Windows Of Blooms Can Be Observed In the Northwestmentioning

confidence: 99%

Section: Different Time Windows Of Blooms Can Be Observed In the Northwestmentioning

confidence: 99%

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Toxin content of Ostreopsis cf. ovata depends on bloom phases, depth and macroalgal substrate in the NW Mediterranean Sea

et al. 2020

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“…cf. ovata (Pistocchi et al ., 2011; Pezzolesi et al ., 2012; Scalco et al ., 2012; Accoroni and Totti, 2016; Carnicer et al ., 2016). Thus, being less adapted to environmental conditions of the Bay of Biscay than O .…”

Section: Discussionmentioning

confidence: 99%

Current distribution and potential expansion of the harmful benthic dinoflagellateOstreopsiscf.siamensistowards the warming waters of the Bay of Biscay,North‐EastAtlantic

Drouet

Jauzein

Herviot-Heath

et al. 2021

Environmental Microbiology

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In a future scenario of increasing temperatures in North-Atlantic waters, the risk associated with the expansion of the harmful, benthic dinoflagellate Ostreopsis cf. siamensis has to be evaluated and monitored. Microscopy observations and spatiotemporal surveys of environmental DNA (eDNA) were associated with Lagrangian particle dispersal simulations to: (i) establish the current colonization of the species in the Bay of Biscay, (ii) assess the spatial connectivity among sampling zones that explain this distribution, and (iii) identify the sentinel zones to monitor future expansion. Throughout a sampling campaign carried out in August to September 2018, microscope analysis showed that the species develops in the south-east of the bay where optimal temperatures foster blooms. Quantitative PCR analyses revealed its presence across almost the whole bay to the western English Channel. An eDNA timeseries collected on plastic samplers showed that the species occurs in the bay from April to September. Due to the water circulation, colonization of the whole bay from the southern blooming zones is explained by inter-site connectivity. Key areas in the middle of the bay permit continuous dispersal connectivity towards the north. These key areas are proposed as sentinel zones to monitor O. cf. siamensis invasions towards the presumably warming water of the North-East Atlantic.

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“…Only O. heptagona is easily distinguishable under a light microscope because the 2′′′′ plate narrows toward the centre of the hypotheca. Moreover, cell sizes overlap among spe-cies, and considerable infraspecific variability in cell diameter has been observed both in field samples and in cultures (Aligizaki and Nikolaidis 2006, David et al 2013, Carnicer et al 2016b.…”

Section: Introductionmentioning

confidence: 99%

Ostreopsis cf. ovata and Ostreopsis lenticularis (Dinophyceae: Gonyaulacales) in the Galapagos Marine Reserve

et al. 2020

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The genus of benthic dinoflagellates Ostreopsis is of particular interest because some species negatively impact human health and coastal marine ecosystems. Ostreopsis populations from a remote area, such as the Galapagos Marine Reserve with its unique biodiversity, can provide significant data. Samples of epibionthic dinoflagellates were collected from two islands (Santa Cruz and Santa Fé) in 2017. Species of the genera Gambierdiscus, Amphidinium, Coolia and Ostreopsis were found. Ostreopsis strains were isolated to characterize their morphology, molecular biology and toxicity. Three different morphotypes of Ostreopsis based on dorsoventral and width diameters (n=369) were distinguished. The small cell morphotype was dominant in ten samples, with abundances of up to 33405 cells g-1 fresh weight of macroalgae. A total of 16 strains were isolated from field samples with subsequent polymerase chain reaction amplifications of rDNA, 5.8S rDNA and internal transcribed space regions; 13 strains (small cell morphotype) clustered in the O. cf. ovata Atlantic/Indian/Pacific clade; and 3 strains (large cell morphotype) clustered in the Ostreopsis lenticularis genotype from the type locality. The strains proved to be non-toxic. The presence of these genera/species represents a potential threat to marine ecosystems, and it is thus important to consider benthic species in the surveillance of harmful algae blooms in the reserve.

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Ostreopsis cf. ovata from western Mediterranean Sea: Physiological responses under different temperature and salinity conditions

Cited by 28 publications

References 54 publications

Toxin content of Ostreopsis cf. ovata depends on bloom phases, depth and macroalgal substrate in the NW Mediterranean Sea

Toxin content of Ostreopsis cf. ovata depends on bloom phases, depth and macroalgal substrate in the NW Mediterranean Sea

Current distribution and potential expansion of the harmful benthic dinoflagellateOstreopsiscf.siamensistowards the warming waters of the Bay of Biscay,North‐EastAtlantic

<em>Ostreopsis</em> cf. <em>ovata</em> and <em>Ostreopsis lenticularis</em> (Dinophyceae: Gonyaulacales) in the Galapagos Marine Reserve

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