Östrogenartige wirkungen von bisphenol a auf vorderkiemenschnecken (Mollusca: Gastropoda: Prosobranchia)

Does bisphenol a induce superfeminization in Marisa cornuarietis? Part I: Intra- and inter-laboratory variability in test endpoints

“…Adult M. cornuarietis used for the experiments reported here showed generally higher reproductive outputs than those reported by Oehlmann et al (2000), Schulte-Oehlmann et al (2001), and Oehlmann et al (2006a), but which are consistent with other published reports (Haridi et al, Estimates of the growth rates in wet weight (g/day) and in diameter (mm/day) at 60 days post-hatch (dph) and of the wet weight and diameter at 60 dph at 25°C for all three laboratories and at 22°C for ABC only.…”

“…Values greater than 100% occurred in some egg masses as a result of eggs containing multiple embryos. ber of advantages over group housing (as used by Oehlmann et al, 2000Oehlmann et al, , 2006aSchulte-Oehlmann et al, 2001). First, it can be determined whether applied treatments affect all individuals similarly, or only a sensitive subgroup of the population.…”

“…Although much has been published on its biology and ecology, there are a number of problems with using M. cornuarietis in ecotoxicological testing that need to be addressed in order to confirm whether BPA has effects on this spe-cies at environmentally relevant concentrations. These problems include a lack of basic knowledge of the influence of husbandry conditions on the performance of M. cornuarietis and the variability of its life-history traits under laboratory conditions, no replication of BPA treatments in most of the published experiments with this species (i.e., Schulte-Oehlmann et al, 2001;Oehlmann et al, 2000, 2006a, associated with the application of inappropriate statistical techniques, that make claims of statistical significance highly questionable (Dietrich et al, 2006, cf., Oehlmann et al, 2006b.…”

“…Several recent papers by Oehlmann and colleagues have claimed that BPA induces superfeminization in the gastropod Marisa cornuarietis. These effects include formation of additional female organs, enlarged accessory sex glands, gross malformations of the pallial oviduct, and a stimulation of egg and egg mass production resulting in increased female mortality (Oehlmann et al, 2000(Oehlmann et al, , 2006aSchulte-Oehlmann et al, 2001). If these claims are valid, they can have an important influence on the risk assessment for this chemical, since, on the basis of the above results, M. cornuarietis would seem to be more sensitive than all other aquatic invertebrates so far tested.…”

Does bisphenol A induce superfeminization in Marisa cornuarietis? Part II: Toxicity test results and requirements for statistical power analyses

“…under laboratory conditions. In addition, the published studies claiming effects of BPA on this species (Oehlmann et al, 2000(Oehlmann et al, , 2006aSchulte-Oehlmann et al, 2001) have been hotly debated (Dietrich et al, 2006, Oehlmann et al, 2006b). The present study aims to contribute new data to this ongoing debate.…”