Otimização de um protocolo para micropropagação da oliveira Ascolano 315

Villa, Fabíola; Pasqual, Moacir; Freitas, Gustavo de Faria

doi:10.1590/s0034-737x2010000400015

Cited by 4 publications

(4 citation statements)

References 15 publications

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“…Similar observation was also reported in olive cultivar 'MGSASC315' by VILLA et al (2010). The same variety (MGSASC315) included in our study the also perform well with 100ml L -1 of coconut water in agreement with the conclusion made by VILLA et al (2010). These authors also showed the positive effect of the coconut water in growth and development of the seedling during in vitro culture.…”

Section: Resultssupporting

confidence: 92%

“…The strong and positive coefficient of determination presented in this work showed the general trend indicating the increase in coconut water dose from 0 to 100ml L -1 is positively associated with the increase in shoot length, number of leaves and number of internodes which indirectly leads us to conclude about the positive effect of coconut water in the seedling development and growth. Similar observation was also reported in olive cultivar 'MGSASC315' by VILLA et al (2010). The same variety (MGSASC315) included in our study the also perform well with 100ml L -1 of coconut water in agreement with the conclusion made by VILLA et al (2010).…”

Section: Resultssupporting

confidence: 92%

“…TREVISAN et al (2005) also demonstrated the advantage of coconut water for stem elongation and plant development in passion fruit. VILLA et al (2010) working with the olive cultivar 'Ascolano 315' reported that 25ml L -1 of coconut water associated with 500µg L -1 of BAP generated plantlets with higher shoot length and heavier fresh biomass than in plantlets cultivated without these compounds.…”

Section: Introductionmentioning

confidence: 99%

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Effect of coconut water on growth of olive embryos cultured in vitro

et al. 2013

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The experiment was carried out to determine the appropriate dose of coconut water as supplement for in vitro cultivation of zygotic embryos from 19 olive genotypes. The isolated embryos of the olive seeds were immersed on culture medium containing 0 (control), 25, 50, and 100mL L-1 of fresh and sterile coconut water and kept for 45 days under controlled environment. The percentage of germination, shoot length, number of roots, number of leaves and number of internodes were measured for all 19 olive genotypes. The ANOVA of the parameters evaluated showed significant genotypes x doses of coconut water interaction for shoot length, number of leaves and number of internodes and the dose of 100mL L-1 produced the best results overall as indicated by the means of measured parameters. However, the study showed the importance of determining the appropriate dose of coconut water for each genotype under consideration as shown by significant genotype x dose of coconut water interaction effect.

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Section: Resultssupporting

confidence: 92%

Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

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Effect of coconut water on growth of olive embryos cultured in vitro

et al. 2013

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“…The same pattern was seen in in vitro cultivated olive embryos, where CW supplementation enhanced the number of leaves but did not affect shoot length or other features (de Souza et al, 2013). The discrepancies in plantlet responses could be related to the CW treatment's reaction being relatively species-specific (Villa et al, 2010).…”

Section: Shoot Multiplication Of M Pudica In Response To Different Pl...supporting

confidence: 52%

Revisiting In vitro Micropropagation Protocols of Mimosa pudica for Enhanced Seed Germination, Shoot Multiplication, and Root Initiation

Baharuddin¹,

Roslan²,

Ramli³

et al. 2023

JTAS

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Mimosa pudica is a medicinal plant worthy of therapeutic properties. It is often overlooked as one of the weed species, and the potential was underappreciated despite its abundance in nature, particularly in tropical climate countries. Considering the aptitude of this species, the micropropagation protocol of M. pudica was revisited and enhanced. The seed surface sterilization and germination were assessed, followed by shoot multiplication rate and root initiation efficiency. Seeds of M. pudica were best surface sterilized with 35% of Clorox and recorded the highest germination rate at 65.55% in media of three-quarter strength Murashige and Skoog (MS) combined with 30 g of sucrose. The explants were harvested and subcultured into multiplication media fortified with 6-benzylaminopurine, kinetin, and coconut water. The highest number of new shoots and leaves were obtained at 3 shoots and 3.27 cm in media of MS combined with 17.6 μm kinetin. Half-strength MS media devoid of hormone was recommended for root induction, resulting in the 5 healthy roots at 2.06 cm after 4 weeks of culture. Overall, the developed micropropagation protocol of M. pudica could facilitate its large-scale cultivation, indicating its potential as a medicinal crop for the extraction of bioactive compounds.

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Rooting Enhancers in the Production of Bougainvillea Seedlings (Bougainvillea Sp.)

Rodrigues

Chagas

Santos

et al. 2020

Int J Innov Educ Res

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This work aimed to evaluate the effect of different rooting enhancers on the development of Bougainvillea cuttings (Bougainvillea spetabilis Willd), aiming to accelerate rhizogenesis for seedling production. Semi-hardwood cuttings standardized in fifteen centimeters in length and diameter of approximately one centimeter were used. After collected, one third of the basal part of cuttings was immersed according to the following treatments: water (control); commercial product (RADIMAXi 20® at concentrations: Ca 25.6%, S 1.8%, Zn 2.5%, Co 1.5%); coconut water (Cocos nucifera L.); Tiririca extract (Cyperus rotundus L.) and Lentil extract (Lensculinaris Medik), for about two seconds. After immersion, cuttings were planted in polyethylene bags filled with 50% of Carolina Soil® commercial substrate and 50% of washed sand, packed under screen (50%) with a micro sprinkler irrigation system. The experimental design used was completely randomized, with five treatments and ten replicates. Results were submitted to analysis of variance and the means of treatments to Student's t test at 5% probability. Evaluations were carried out at 45 days after installation and variables analyzed were: number of sprouts per cutting, length of the largest sprout, percentage of rooted cuttings and non-rooted live cuttings, length of the largest root, fresh and dry matter of roots, fresh and dry matter of shoots. Treatments that showed statistical difference were lentil extract, with the highest number of sprouts and the highest percentage of rooted cuttings and non-rooted live cuttings, and treatment with coconut water obtained the highest result in relation to variable length of the largest sprout, standing out as a promising sustainable alternative in the vegetative production of Bougainvillea seedlings.

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Otimização de um protocolo para micropropagação da oliveira Ascolano 315

Cited by 4 publications

References 15 publications

Effect of coconut water on growth of olive embryos cultured in vitro

Effect of coconut water on growth of olive embryos cultured in vitro

Revisiting In vitro Micropropagation Protocols of Mimosa pudica for Enhanced Seed Germination, Shoot Multiplication, and Root Initiation

Rooting Enhancers in the Production of Bougainvillea Seedlings (Bougainvillea Sp.)

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