Otolith fluorescent and thermal marking of elongate loach (Leptobotia elongata) at early life stages

Yang, Kun; Zeng, Rukui; Gan, Weixiong; Deng, Longjun; Song, Zhaobin

doi:10.1007/s10641-016-0509-6

“…Among these techniques, otolith marking is a feasible method that allows long-term identification of small fish ( Caraguel et al, 2015 ; Crook et al, 2009 ). Otolith marks can be achieved by fluorescent marking ( Yang et al, 2016 ), thermal marking ( Volk, Schroder & Grimm, 1999 ) and isotopic marking ( Woodcock et al, 2011 ). The most popular marking protocol is to use fluorochromes, which can form chelate complexes with calcium ions that are built into skeletal and otolith structures ( Poczyczyński et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Mass marking of juvenileSchizothorax wangchiachii(Fang) with alizarin red S and evaluation of stock enhancement in the Jinping area of the Yalong River

Yang

¹

,

Li

²

,

Liu

³

et al. 2017

PeerJ

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Schizothorax wangchiachii is a key fish species in the stock enhancement program of the Yalong River hydropower project, China. Alizarin red S (ARS) was used to mark large numbers of juvenile S. wangchiachii in the Jinping Hatchery and later used to evaluate stock enhancement in the Jinping area of the Yalong River. In a small-scale pilot study, 7,000 juveniles of the 2014 cohort were successfully marked by immersion in ARS solution, and no mortality was recorded during the marking process. The ARS mark in the fish otoliths remained visible 20 months later. In the large-scale marking study, approximately 600,000 juveniles of the 2015 cohort were successfully marked. Mortalities of both marked and unmarked juveniles were very low and did not differ significantly. Total length, wet mass and condition factor did not differ significantly between unmarked and marked individuals after three months. On 24 July 2015, about 840,000 Jinping Hatchery-produced young S. wangchiachii, including 400,000 marked individuals, were released at two sites in the Jinping area. Recapture surveys showed that (1) marked and unmarked S. wangchiachii did not differ significantly in total length, wet mass and condition factor; (2) stocked individuals became an important part of recruitment of the 2015 cohort; (3) instantaneous growth rate of marked individuals tended to slightly increase; and (4) most stocked individuals were distributed along a 10–15 km stretch near the release sites. These results suggest that the ARS method is a cost-efficient way to mass mark juvenile S. wangchiachii and that releasing juveniles is an effective means of stock recruitment.

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“…Among these techniques, otolith marking is a feasible method that allows long-term identification of small fish (Caraguel et al, 2014;Crook et al, 2009). Otolith marks can be achieved by fluorescent marking (Yang et al, 2016), thermal marking (Volk et al, 1999) and isotopic marking (Woodcock et al, 2011). The most popular marking protocol is to use fluorochromes, which can form chelate complexes with calcium ions that are built into skeletal and otolith structures (Poczyczyński et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…The most popular marking protocol is to use fluorochromes, which can form chelate complexes with calcium ions that are built into skeletal and otolith structures (Poczyczyński et al, 2011). Fluorescent marks on the otolith are visible under a specific inducing laser because the calcium-fluorochrome complexes emit fluorescent light (Bashey, 2004;Taylor et al, 2005;Yang et al, 2016). Fluorochromes commonly used for otolith marking are alizarin red S (ARS), alizarin complexone, oxytetracycline hydrochloride and calcein.…”

Section: Introductionmentioning

confidence: 99%

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Peer Review #2 of "Mass marking of juvenile Schizothorax wangchiachii (Fang) with alizarin red S and evaluation of stock enhancement in the Jinping area of the Yalong River (v0.1)"

2017

0

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“…The most popular marking protocol is to use fluorochromes, which can form chelate complexes with calcium ions that are built into skeletal and otolith structures (Poczyczyński et al, 2011). Fluorescent marks on the otolith are visible under a specific inducing laser because the calcium-fluorochrome complexes emit fluorescent light (Bashey, 2004;Taylor et al, 2005;Yang et al, 2016). Fluorochromes commonly used for otolith marking are alizarin red S (ARS), alizarin complexone, oxytetracycline hydrochloride and calcein.…”

Section: Introductionmentioning

confidence: 99%

Peer Review #1 of "Mass marking of juvenile Schizothorax wangchiachii (Fang) with alizarin red S and evaluation of stock enhancement in the Jinping area of the Yalong River (v0.1)"

Cottingham¹

2017

0

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Otolith fluorescent and thermal marking of elongate loach (Leptobotia elongata) at early life stages

Cited by 6 publications

References 25 publications

Mass marking of juvenileSchizothorax wangchiachii(Fang) with alizarin red S and evaluation of stock enhancement in the Jinping area of the Yalong River

Mass marking of juvenileSchizothorax wangchiachii(Fang) with alizarin red S and evaluation of stock enhancement in the Jinping area of the Yalong River

Peer Review #2 of "Mass marking of juvenile Schizothorax wangchiachii (Fang) with alizarin red S and evaluation of stock enhancement in the Jinping area of the Yalong River (v0.1)"

Peer Review #1 of "Mass marking of juvenile Schizothorax wangchiachii (Fang) with alizarin red S and evaluation of stock enhancement in the Jinping area of the Yalong River (v0.1)"

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