Ouabain impairs cell migration, and invasion and alters gene expression of human osteosarcoma <scp>U</scp>‐2 <scp>OS</scp> cells

Shih, Yu‐Lueng; Au, Man Kuan; Liu, Ko Lin; Yeh, Ming‐Yang; Lee, Ching Hsiao; Lee, Mei Hui; Lu, Hsu Feng; Yang, Jinghong; Wu, Rick Sai Chuen; Chung, Jing Gung

doi:10.1002/tox.22453

Cited by 17 publications

(28 citation statements)

References 47 publications

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“…When natural products affect cancer cells at high concentrations, this can be explained by their potency to induce apoptosis 21 . However, low cytotoxic concentrations of compounds are commonly chosen for migration studies 38–40 . As for POMx, at low cytotoxic concentrations it also shows wound healing inhibition in breast cancer stem cells 16 .…”

Section: Discussionmentioning

confidence: 99%

Pomegranate extract inhibits migration and invasion of oral cancer cells by downregulating matrix metalloproteinase‐2/9 and epithelial‐mesenchymal transition

Peng

Hsiao

Lan

et al. 2020

Environmental Toxicology

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Discovering drug candidates for the modulation of metastasis is of great importance in inhibiting oral cancer malignancy. Although most pomegranate extract applications aim at the antiproliferation of cancer cells, its antimetastatic effects remain unclear, especially for oral cancer cells. The aim of this study is to evaluate the change of two main metastasis characters, migration and invasion of oral cancer cells. Further, we want to explore the molecular mechanisms of action of pomegranate extract (POMx) at low cytotoxic concentration. We found that POMx ranged from 0 to 50 μg/mL showing low cytotoxicity to oral cancer cells. In the case of oral cancer HSC-3 and Ca9-22 cells, POMx inhibits wound healing migration, transwell migration, and matrix gel invasion.Mechanistically, POMx downregulates matrix metalloproteinase (MMP)-2 and MMP-9 Sheng-Yao Peng and Chien-Chou Hsiao contributed equally to this work.

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Section: Discussionmentioning

confidence: 99%

Pomegranate extract inhibits migration and invasion of oral cancer cells by downregulating matrix metalloproteinase‐2/9 and epithelial‐mesenchymal transition

Peng

Hsiao

Lan

et al. 2020

Environmental Toxicology

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“…In vitro scratch wound healing assay for cell mobility. Scratch wound healing assay was used to examine cell mobility characteristics as previously described (28). Briefly, U-2 OS cells (1×10 5 cells/well) were grown in a 12-well plate until they reached a confluent monolayer.…”

Section: Methodsmentioning

confidence: 99%

“…Image J software was used to quantify the relative wound size. Cell mobility inhibition (%)=new scratch width/original scratch width ×100% as previously described (28,29).…”

Section: Methodsmentioning

confidence: 99%

“…harvested and loaded onto 10% polyacrylamide gels and copolymerized with 0.2% gelatin. Gel was soaked twice in 2.5% Triton X-100 in dH 2 O at 25˚C for 30 min as previously described (28,30). Then, gel was incubated in zymogen developing buffer (Sigma Aldrich), containing 50 mM Tris (pH 7.5), 200 mM NaCl, 5 mM CaCl 2 , 1 μM ZnCl 2 and 0.02% Brij 35, overnight at 37˚C.…”

Section: Methodsmentioning

confidence: 99%

“…Cell migration and invasion in vitro were examined by using Collagen and Matrigel assay system as previously described (28,30). Briefly, U-2 OS cells (5×10 4 cells/well) in serum-free McCoy's 5A culture medium containing different concentrations of fisetin (0, 2.5, 5 and 10 μM) were placed in the upper chamber (transwell insert) (8 μm pore size; Millipore, Temecula, CA, USA) which was coated with 50 μl collagen (for cell migration examination) overnight.…”

Section: Cell Migration and Invasion Analyzed With The Transwell Assaymentioning

confidence: 99%

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Fisetin Suppresses Human Osteosarcoma U-2 OS Cell Migration and InvasionviaAffecting FAK, uPA and NF-ĸB Signaling PathwayIn Vitro

Chen

Peng

Lai

et al. 2019

In Vivo

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Background/Aim: Evidence has indicated that fisetin induces cytotoxic effects in human cancer cell lines, including the inhibition of cell migration and invasion, however, the exact molecular mechanism of action of fisetin in human osteosarcoma cells remains unclear. Materials and Methods: The anti-metastatic mechanisms of fisetin in human osteosarcoma U-2 OS cells were investigated in vitro. Results: Fisetin reduced the viability of cells at different concentrations (2.5, 5 and 10 μM) as measured by flow cytometric assay. Fisetin suppressed cell mobility, migration and invasion of U-2 OS cells, as shown by wound healing assay and transwell filter chambers, respectively. The gelatin zymography assay showed that fisetin inhibited MMP-2 activity in U-2 OS cells. Results from western blotting indicated that fisetin reduced the levels

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In vitro antitumoral effects of the steroid ouabain on human thyroid papillary carcinoma cell lines

Teixeira

Passos

Haddad

et al. 2021

Environmental Toxicology

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Ouabain is a steroid described as a compound extracted from plants that is capable of binding to Na+, K+‐ATPase, inhibiting ion transport and triggering cell signaling pathways. Due to its positive ionotropic effect, ouabain was used for more than 200 years for the treatment of cardiac dysfunctions. Numerous antitumor effects of ouabain have been described so far; however, its role on thyroid cancer is still poorly understood. Therefore, the aim of the present work was to evaluate the effect of ouabain on the biology of human papillary thyroid cancer cells. For this, three human thyroid cell lines were used: NTHY‐ori, a non‐tumor lineage, BCPAP and TPC‐1, both derived from papillary carcinomas. Cells were cultured in the presence or absence of ouabain. Subsequently, we evaluated its effects on the viability, cell death, cell cycle, and migratory ability of these cell lines. We also investigated the impact of ouabain in IL‐6/IL‐6R and epithelial to mesenchymal transition markers expression. Our results indicate that ouabain (10−7 M), decreased the number of NTHY‐ori, TPC‐1 and BCPAP viable cells and induced cell cycle arrest after in vitro culture, but did not appear to promote cell death. In TPC‐1 cells ouabain also inhibited cell migration; increased IL‐6/IL‐6R expression and IL‐6 secretion; and diminished vimentin and SNAIL‐1 expression. Collectively, our results indicate that ouabain has an antitumoral role on human papillary thyroid carcinomas in vitro. Even though additional studies are necessary, our work contributes to the discussion of the possibility of new clinical trials of ouabain.

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Ouabain impairs cell migration, and invasion and alters gene expression of human osteosarcoma U‐2 OS cells

Cited by 17 publications

References 47 publications

Pomegranate extract inhibits migration and invasion of oral cancer cells by downregulating matrix metalloproteinase‐2/9 and epithelial‐mesenchymal transition

Pomegranate extract inhibits migration and invasion of oral cancer cells by downregulating matrix metalloproteinase‐2/9 and epithelial‐mesenchymal transition

Fisetin Suppresses Human Osteosarcoma U-2 OS Cell Migration and InvasionviaAffecting FAK, uPA and NF-ĸB Signaling PathwayIn Vitro

In vitro antitumoral effects of the steroid ouabain on human thyroid papillary carcinoma cell lines

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