Ouabain-sensitive (Na+ + K+)-ATPase activity expressed in mouse L cells by transfection with DNA encoding the alpha-subunit of an avian sodium pump.

Takeyasu, Kunio; Tamkun, Michael M.; Renaud, K J; Fambrough, Douglas M.

doi:10.1016/s0021-9258(18)68932-x

Cited by 304 publications

(28 citation statements)

References 44 publications

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“…To assess whether the localization of mutant pumps was different from WT pumps, we conducted immunofluorescence studies using Xpress-tagged (Invitrogen) WT, WT-OR, and mutant OR ␣3 constructs transiently transfected into HEK293 cells and found no remarkable differences (data not shown). In accordance with other studies (Takeyasu et al, 1988) in which exogenous ␣ subunit is expressed without additional exogenous ␤ subunit, much of the nascent a3 appears in ER and Golgi, but some was observed at the plasma membrane for all mutant and WT constructs. Qualitatively, this indicates that none of the mutations disrupted the maturation of the Na ϩ /K ϩ -ATPase.…”

Section: Department Ofsupporting

confidence: 92%

Mutations in the Na+/K+-ATPase α3 Gene ATP1A3 Are Associated with Rapid-Onset Dystonia Parkinsonism

Aguiar

Sweadner

Penniston

et al. 2004

Neuron

471

343

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Rapid-onset dystonia-parkinsonism (RDP, DYT12) is a distinctive autosomal-dominant movement disorder with variable expressivity and reduced penetrance characterized by abrupt onset of dystonia, usually accompanied by signs of parkinsonism. The sudden onset of symptoms over hours to a few weeks, often associated with physical or emotional stress, suggests a trigger initiating a nervous system insult resulting in permanent neurologic disability. We report the finding of six missense mutations in the gene for the Na+/K+ -ATPase alpha3 subunit (ATP1A3) in seven unrelated families with RDP. Functional studies and structural analysis of the protein suggest that these mutations impair enzyme activity or stability. This finding implicates the Na+/K+ pump, a crucial protein responsible for the electrochemical gradient across the cell membrane, in dystonia and parkinsonism.

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Section: Department Ofsupporting

confidence: 92%

Mutations in the Na+/K+-ATPase α3 Gene ATP1A3 Are Associated with Rapid-Onset Dystonia Parkinsonism

Aguiar

Sweadner

Penniston

et al. 2004

Neuron

471

343

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“…(2007). Sections were double labelled with mouse and rabbit antibodies against NKA (rabbit polyclonal NAK121) (Wilson et al ., 2007) and a5 mouse monoclonal (Takeyasu et al ., 1988), NKCC (T4 mouse monoclonal (Lytle et al ., 1995) and CFTR (C‐term mouse monoclonal; R&D Systems). Alexa Fluor secondary antibodies (goat anti‐rabbit 488 and goat anti‐mouse 555) were used (Thermo Scientific).…”

Section: Methodsmentioning

confidence: 99%

Freshening effect on the osmotic response of the Antarctic spiny plunderfish Harpagifer antarcticus

Vargas‐Chacoff

Dann

Paschke

et al. 2021

Journal of Fish Biology

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Global warming is having a significant impact around the world, modifying environmental conditions in many areas, including in zones that have been thermally stable for thousands of years, such as Antarctica. Stenothermal sedentary intertidal fish species may suffer due to warming, notably if this causes water freshening from increased freshwater inputs. Acute decreases in salinity, from 33 down to 5, were used to assess osmotic responses to environmental salinity fluctuations in Antarctic spiny plunderfish Harpagifer antarcticus, in particular to evaluate if H. antarcticus is able to cope with freshening and to describe osmoregulatory responses at different levels (haematological variables, muscle water content, gene expression, NKA activity). H. antarcticus were acclimated to a range of salinities (33 as control, 20, 15, 10 and 5) for 1 week. At 5, plasma osmolality and calcium concentration were both at their lowest, while plasma cortisol and percentage muscle water content were at their highest. At the same salinity, gill and intestine Na + -K + -ATPase (NKA) activities were at their lowest and highest, respectively. In kidney, NKA activity was highest at intermediate salinities (15 and 10). The salinity-dependent NKA mRNA expression patterns differed depending on the tissue. Marked changes were also observed in the expression of genes coding membrane proteins associated with ion and water transport, such as NKCC2, CFTR and AQP8, and in the expression of mRNA for the regulatory hormone prolactin (PRL) and its receptor (PRLr). Our results demonstrate that freshening causes osmotic imbalances in H. antarcticus, apparently due to reduced capacity of both transport and regulatory mechanisms of key organs to maintain homeostasis. This has implications for fish species that have evolved in stable environmental conditions in the Antarctic, now threatened by climate change.

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“…Immunolocalization of the Na + ,K + -ATPase was performed through immunofluorescence light microscopy using a mouse monoclonal antibody IgGα 5 raised against the α-subunit of the chicken Na + ,K + -ATPase (Takeyasu et al 1988) obtained from the Development Studies Hybridoma Bank, developed under the auspices of the NICHD, and maintained by the University of Iowa. In crustaceans, this antiserum has previously been used to quantify the expression of the α subunit after a dilute transfer in Carcinus maenas (Lucu and Flik 1999) and to localize the Na + ,K + -ATPase in the branchial cavity of Homarus gammarus (Lignot and Charmantier 1999; Lignot et al 2001) and in the gills (Lignot et al 2005) and antennal glands (Khodabandeh et al 2005b) of Astacus leptodactylus.…”

Section: Methodsmentioning

confidence: 99%

Ultrastructural Studies and Na⁺,K⁺-ATPase Immunolocalization in the Antennal Urinary Glands of the Lobster Homarus gammarus (Crustacea, Decapoda)

Khodabandeh

Charmantier

Charmantier‐Daures

2005

J Histochem Cytochem.

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S U M M A R YUnlike in crustacean freshwater species, the structure and ultrastructure of the excretory antennal gland is poorly documented in marine species. The general organization and ultrastructure of the cells and the localization of Na ϩ ,K ϩ -ATPase were examined in the antennal gland of the adult lobster Homarus gammarus . Each gland is composed of a centrally located coelomosac surrounded ventrally by a labyrinth divided into two parts (I and II) and dorsally by a voluminous bladder. There is no differentiated nephridal tubule between them. The labyrinth and bladder cells have in common a number of ultrastructural cytological features, including basal membrane infoldings associated with mitochondria, apical microvilli, and cytoplasmic extrusions, and a cytoplasm packed with numerous vacuoles, vesicles, lysosome-like bodies, and swollen mitochondria. Each type of cell also presents distinctive characters. Na ϩ ,K ϩ -ATPase was detected through immunofluorescence in the basal part of the cells of the labyrinth and in the bladder cells with an increasing immunostaining from labyrinth I to the bladder.

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Ouabain-sensitive (Na+ + K+)-ATPase activity expressed in mouse L cells by transfection with DNA encoding the alpha-subunit of an avian sodium pump.

Cited by 304 publications

References 44 publications

Mutations in the Na+/K+-ATPase α3 Gene ATP1A3 Are Associated with Rapid-Onset Dystonia Parkinsonism

Mutations in the Na+/K+-ATPase α3 Gene ATP1A3 Are Associated with Rapid-Onset Dystonia Parkinsonism

Freshening effect on the osmotic response of the Antarctic spiny plunderfish Harpagifer antarcticus

Ultrastructural Studies and Na⁺,K⁺-ATPase Immunolocalization in the Antennal Urinary Glands of the Lobster Homarus gammarus (Crustacea, Decapoda)

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Ouabain-sensitive (Na+ + K+)-ATPase activity expressed in mouse L cells by transfection with DNA encoding the alpha-subunit of an avian sodium pump.

Cited by 304 publications

References 44 publications

Mutations in the Na+/K+-ATPase α3 Gene ATP1A3 Are Associated with Rapid-Onset Dystonia Parkinsonism

Mutations in the Na+/K+-ATPase α3 Gene ATP1A3 Are Associated with Rapid-Onset Dystonia Parkinsonism

Freshening effect on the osmotic response of the Antarctic spiny plunderfish Harpagifer antarcticus

Ultrastructural Studies and Na+,K+-ATPase Immunolocalization in the Antennal Urinary Glands of the Lobster Homarus gammarus (Crustacea, Decapoda)

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Ultrastructural Studies and Na⁺,K⁺-ATPase Immunolocalization in the Antennal Urinary Glands of the Lobster Homarus gammarus (Crustacea, Decapoda)