Out of sequence

Baum, Bobby

doi:10.1038/305090a0

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“…Recently several approaches have been pursued to automate part(s) of this process (3,4,5,6,7 and references therein) in an attempt to increase the throughput and free the researcher from this labor intensive "error prone" task. This has essentially removed certain sources of procedural, but not interpretive errors (8). Although automation has minimized these sources of error, the accuracy rates reported for the automated sequencing machines varies from 94 % to 99 % (3,4,5,6) and is dependent on the optimum sensitivity threshold of detection (7).…”

Section: Introductionmentioning

confidence: 99%

Sequence errors described in GenBank: a means to determine the accuracy of DNA sequence interpretation

Krawetz

1989

Nucl Acids Res

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The accuracy of nucleic acid sequence data interpretation was determined by assessing and quantifying the discrepancies reported in the GenBank database. This permitted the calculation of an Error Rate (ER) for nucleic acid sequence determination. If one assumes that most entries (TB, Total Bases) were independently verified or those without reported discrepancies were correct, the ER is 0.368 errors per 1000 bases. However, if one assumes that only those sequences with reported discrepancies (TBIQ, Total Bases from entries In Question) were verified and are thus correct, the ER is 2.887 errors per 1000 bases. This establishes the first set of limit boundaries of the ER for sequence interpretation and sequence errors within the GenBank database and provides the foundation for future assessments and the monitoring of sequence data accumulation. In addition, the ER measure provides a basis to evaluate the efficiency and merit of present and future automated nucleic acid sequencing technologies which will have a direct impact upon the final outcome of the "Human Genome Initiative".

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Section: Introductionmentioning

confidence: 99%

Sequence errors described in GenBank: a means to determine the accuracy of DNA sequence interpretation

Krawetz

1989

Nucl Acids Res

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Out of sequence

Cited by 1 publication

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Sequence errors described in GenBank: a means to determine the accuracy of DNA sequence interpretation

Sequence errors described in GenBank: a means to determine the accuracy of DNA sequence interpretation

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