Outbreak Caused byblaOXA-72-ProducingAcinetobacter baumanniiST417 Detected in Clinical and Environmental Isolates

Tamayo-Legorreta, Elsa; Turrubiartes-Martínez, Edgar; Garza–Ramos, Ulises; Niño-Moreno, Perla; Barrios, H.; Sánchez-Pérez, Alejandro; Reyna-Flores, Fernando; Tovar-Oviedo, Juana; Magaña-Aquino, Martín; Cevallos, Miguel A.; Silva-Sánchez, Jesús

doi:10.1089/mdr.2015.0157

Cited by 8 publications

(7 citation statements)

References 27 publications

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“…With respect to the A. baumannii populations in Mexico, we are adamant that our study could be of paramount importance for future studies tackling the population genomics of this species in this country. Clearly this is not the first study analyzing A. baumannii isolates from Mexico; in fact, over the last decade there have been several studies (Ares et al, 2013 ; Morfin-Otero et al, 2013 ; Alcantar-Curiel et al, 2014 ; Bocanegra-Ibarias et al, 2015 ; Cornejo-Juarez et al, 2015 ; Gonzalez-Villoria et al, 2016 ; Tamayo-Legorreta et al, 2016 ), which have been very useful from a clinical point of view, as they focused on the molecular epidemiology (Morfin-Otero et al, 2013 ; Gonzalez-Villoria et al, 2016 ; Tamayo-Legorreta et al, 2016 ) or the antibiotic resistance profiles of hospital isolates (Ares et al, 2013 ; Alcantar-Curiel et al, 2014 ; Bocanegra-Ibarias et al, 2015 ). However, none of those studies have analyzed the genomic diversity of the isolates in question and, to the best of our knowledge, this is the first study that has addressed the genetic variation within a population of A. baumannii isolates in Mexico at a genome level.…”

Section: Discussionmentioning

confidence: 93%

Rapid Gene Turnover as a Significant Source of Genetic Variation in a Recently Seeded Population of a Healthcare-Associated Pathogen

et al. 2017

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Genome sequencing has been useful to gain an understanding of bacterial evolution. It has been used for studying the phylogeography and/or the impact of mutation and recombination on bacterial populations. However, it has rarely been used to study gene turnover at microevolutionary scales. Here, we sequenced Mexican strains of the human pathogen Acinetobacter baumannii sampled from the same locale over a 3 year period to obtain insights into the microevolutionary dynamics of gene content variability. We found that the Mexican A. baumannii population was recently founded and has been emerging due to a rapid clonal expansion. Furthermore, we noticed that on average the Mexican strains differed from each other by over 300 genes and, notably, this gene content variation has accrued more frequently and faster than the accumulation of mutations. Moreover, due to its rapid pace, gene content variation reflects the phylogeny only at very short periods of time. Additionally, we found that the external branches of the phylogeny had almost 100 more genes than the internal branches. All in all, these results show that rapid gene turnover has been of paramount importance in producing genetic variation within this population and demonstrate the utility of genome sequencing to study alternative forms of genetic variation.

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Section: Discussionmentioning

confidence: 93%

Rapid Gene Turnover as a Significant Source of Genetic Variation in a Recently Seeded Population of a Healthcare-Associated Pathogen

et al. 2017

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“…ST219 infection was observed in a hospital in Japan [10]. The clonal spread of ST417 occurred in one hospital in Mexico, and some novel STs, such as ST758, ST762 and ST777 were also reported [5].…”

Section: Discussionmentioning

confidence: 97%

“…More recently, some researches revealed that Acinetobacter baumannii ST369 was implicated in the outbreak occurred in hospitals of China and Korean, but it was not the dominant ST [5,25,26].…”

Section: Discussionmentioning

confidence: 99%

“…Although carbapenems were effective antibiotics for the treatment of Acinetobacter baumannii infection, the efficacy of carbapenems has been reducing with the emergence of multidrug resistant (MDR) strains, especially carbapenem-resistant strains in recent years [2,4]. The bla OXA−type resistance genes, including bla OXA−23−like and bla OXA−72 genes, are mainly responsible for this event due to their ability of hydrolyzing carbapenems, which further facilitates the spread of Acinetobacter baumannii worldwide and increases the challenge for infection control [5][6][7]. To date, outbreaks of bla OXA−type -producing MDR Acinetobacter baumannii have been reported throughout the world, and different sequence type (ST) have been observed in various outbreaks throughout Asia [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

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Extensively Drug Resistant Acinetobacter baumannii ST369 Infection in Emergency Intensive Care Unit in Shandong Province, China

Jiang¹,

L²,

S³

et al. 2020

Preprint

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Background: Acinetobacter baumannii is a significant nosocomial infectious pathogen worldwide. The aim of this study is to characterize the molecular epidemiology of Acinetobacter baumannii isolated from the clinical infection, providing the epidemiology data for prevention and control. Four patients hospitalized in EICU on January 31st, 2014, and then Acinetobacter baumannii infection was observed.Antimicrobial resistance and resistance genes were analyzed by antimicrobial susceptibility testing and PCR sequencing. Pulse field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used to analyze these strains' clonal relatedness.Results: Sixteen strains were recovered, of which 4 strains were isolated from 4 patients, and others were from environment in EICU, such as air, phone and ventilator. All strains belonged to clonal pulsotype A and ST369. Sixteen antibiotics were used to perform the susceptibility testing, and all strains were extensively drug resistant (XDR) Acinetobacter baumannii, they were only susceptible to tigecycline and polymyxin B, but resistant to others, including carbapenems and aminoglycoside antibiotics. Furthermore, all strains carried blaOXA-23-like carbapenemases gene with ISAba1 insertion sequence in the upstream, aminoglycoside resistance genes ant(3″)-I, 16S rRNA methylase gene armA and disinfectant resistant gene qacE△1, which were mainly responsible for the spread of antimicrobial resistance. Fortunately, enhanced control measures were immediately implemented after this infection, and new strains were no longer detected for consecutive three months.Conclusions: molecular epidemiology of blaOXA-23-like carbapenemase-producing Acinetobacter baumannii ST369 in EICU of a hospital was characterized. Routine monitoring should be strengthen to prevent outbreaks of this disease.

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“…Today, the most formidable persister with clinical relevance is a new arrival to the general lists, Acinetobacter baumannii , which was placed number one on the WHO list of “priority one–critical” bacteria [8]. It is followed by long-challenging Pseudomonas aeruginosa, which is also extremely abundant and has enormous genetic flexibility and adaptability.…”

Section: Antimicrobial Drug Resistance (Amr) Crisis: Can Medicine Stimentioning

confidence: 99%

Bacteriophages: A Therapy Concept against Multi-Drug–Resistant Bacteria

Rohde

Wittmann

Kutter

2018

Surgical Infections

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Bacteriophages (phages) are viruses that kill bacteria specifically but cannot infect other kinds of organisms. They have attracted new attention since the increasing antibiotic resistance developed into a global crisis. Phage therapy, a 100-year-old form of antibacterial treatment in medicine, is gaining momentum because phages represent a therapy concept without such negative side effects as toxicity; phages are the only therapeutic agent that regulates itself at the sites of infection and decays when the infectious bacteria have been killed. Nature is an almost infinite phage resource: New ones can be isolated for most kinds of problem bacteria as needed; bacteria and their phages constantly co-evolve. This is important as new pathogenic bacterial variants evolve and new challenging situations arise. In human therapy, “cocktails” of multiple phages may reduce the probability of selecting bacteria that developed resistance to a certain phage. Antibiotic agents can be applied together with phages in many circumstances; the two often function synergistically. Phages cannot be expected to replace antibiotic agents in our medical arsenal, but can be used where antibiotic agents fail. The selected phages, however, must be obligately virulent, well-characterized, and highly purified before application. Countless patients and their physicians are waiting for re-establishing phage therapy as a flexible, tailored medicine; infrastructures should be built in all countries urgently: The 2015 World Health Organization assembly resolution 68.7.3. called for national action plans by May 2017 to combat the antimicrobial drug resistance crisis. This article discusses the therapeutic potential of phages and describes challenges and recent developments.

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Outbreak Caused bybla_OXA-72-ProducingAcinetobacter baumanniiST417 Detected in Clinical and Environmental Isolates

Cited by 8 publications

References 27 publications

Rapid Gene Turnover as a Significant Source of Genetic Variation in a Recently Seeded Population of a Healthcare-Associated Pathogen

Rapid Gene Turnover as a Significant Source of Genetic Variation in a Recently Seeded Population of a Healthcare-Associated Pathogen

Extensively Drug Resistant Acinetobacter baumannii ST369 Infection in Emergency Intensive Care Unit in Shandong Province, China

Bacteriophages: A Therapy Concept against Multi-Drug–Resistant Bacteria

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