Outbreak of CTX-M-15-Producing Enterotoxigenic Escherichia coli O159:H20 in the Republic of Korea in 2016

Kim, Jin Seok; Park, Jungsun; Shin, Eunkyung; Kim, Soojin; Oh, Sung Suck; Yang, Hyo-Jin; Kim, Dae‐Won; Oh, Kyunghwan; Kim, Yong‐Hoon; Kim, Min; Kwon, Mun Ju; Na, Kyoungin; Liu, Jin; Cho, Enhi; Kang, Byunghak; Kwak, Hyo‐Sun; Seong, Won Keun; Kim, Junyoung

doi:10.1128/aac.00339-17

Cited by 9 publications

(5 citation statements)

References 17 publications

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“…Analysis of the Illumina WGS assemblies also revealed a further plasmid-located bla CTX–M–15 for the traveler strain BS74R-A ( Table 2 ). Interestingly, this 95 kb IncK plasmid was almost identical (99% coverage and 98% identity) to a plasmid from an enterotoxigenic Ec that caused an outbreak in Korea in 2016 (data not shown) ( Kim et al, 2017 ).…”

Section: Resultsmentioning

confidence: 97%

“…For these reasons, and to better understand this overall global issue, the WGS methodological approaches used in this study will be essential when surveying other countries. Moreover, in future studies, other potential sources (e.g., water and wildlife) that could play a major role in the dissemination of AMR should be explored ( Kim et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

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Antimicrobial-Resistant Escherichia coli Strains and Their Plasmids in People, Poultry, and Chicken Meat in Laos

et al. 2021

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Antimicrobial resistant (AMR) Enterobacterales are widely distributed among the healthy population of the Indochinese peninsula, including Laos. However, the local reservoir of these pathogens are currently not known and possible sources such as agricultural settings and food have rarely been analyzed. In this work, we investigated the extended-spectrum cephalosporin- (ESC-) and colistin-resistant Escherichia coli strains (CST-R-Ec) isolated from the gut of local people, feces of poultry, and from chicken meat (60 samples each group) in Laos. Whole-genome sequencing (WGS) analysis based on both short- and long-read sequencing approaches were implemented. The following prevalence of ESC-R-Ec and CST-R-Ec were recorded, respectively: local people (70 and 15%), poultry (20 and 23.3%), and chicken meat (21.7 and 13.3%). Core-genome analysis, coupled with sequence type (ST)/core-genome ST (cgST) definitions, indicated that no common AMR-Ec clones were spreading among the different settings. ESC-R-Ec mostly possessed blaCTX–M–15 and blaCTX–M–55 associated to ISEcp1 or IS26. The majority of CST-R-Ec carried mcr-1 on IncX4, IncI2, IncP1, and IncHI1 plasmids similar or identical to those described worldwide; strains with chromosomal mcr-1 or possessing plasmid-mediated mcr-3 were also found. These results indicate a high prevalence of AMR-Ec in the local population, poultry, and chicken meat. While we did not observe the same clones among the three settings, most of the blaCTX–Ms and mcr-1/-3 were associated with mobile-genetic elements, indicating that horizontal gene transfer may play an important role in the dissemination of AMR-Ec in Laos. More studies should be planned to better understand the extent and dynamics of this phenomenon.

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Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Antimicrobial-Resistant Escherichia coli Strains and Their Plasmids in People, Poultry, and Chicken Meat in Laos

et al. 2021

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“…The bla CTX-M-15 gene is located between 9,141 and 10,016 bp on the plasmid, with an orf477 sequence downstream, but none of the transposable elements frequently associated with bla CTX-M were observed in the upstream region. The overall genetic structure of the selected ETEC O25 isolates ( Δbla TEM - bla CTX-M-15 - orf477 ) has been described to be similar to that of pET20160881 (IncK, GenBank accession number KY554955; Figure 4 ) and pSH4469 (IncI1, GenBank accession number KY406378; Figure 4 ; Kim et al, 2014a , 2017 ). The plasmid backbone shares high identity (99.8 and 98.7%, respectively) with these plasmids.…”

Section: Discussionmentioning

confidence: 92%

Co-infection With Chromosomally-Located blaCTX-M-14 and Plasmid-Encoding blaCTX-M-15 in Pathogenic Escherichia coli in the Republic of Korea

et al. 2020

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The emergence of third-generation cephalosporin resistance in Escherichia coli is increasing at an alarming rate in many countries. Thus, the aim of this study was to analyze co-infecting bla CTX-M -producing pathogenic E. coli isolates linked to three school outbreaks. Among 66 E. coli isolates, 44 were identified as ETEC O25, an ETEC isolate serotype was O2, and the other 21 were confirmed as EAEC O44. Interestingly, six patients were co-infected with EAEC O44 and ETEC O25. For these isolates, molecular analysis [antibiotic susceptibility testing, identification of the β-lactamase gene, multilocus sequence typing (MLST), and pulsed-field gel electrophoresis (PFGE)] was performed for further characterization. In addition, the transmission capacity of bla CTX-M genes was examined by conjugation experiments. Whole-genome sequencing (WGS) was performed on representative EAEC O44 and ETEC O25 isolates associated with co-infection and single-infection. All isolates were resistant to cefotaxime and ceftriaxone. All EAEC isolates carried the bla CTX-M-14 gene and all ETEC isolates the bla CTX-M-15 gene, as detected by multiplex PCR and sequencing analysis. Sequence type and PFGE results indicated three different patterns depending on the O serotype. WGS results of representative isolates revealed that the ETEC O25 strains harbored bla CTX-M-15 located on IncK plasmids associated with the Δ bla TEM - bla CTX-M-15 - orf477 transposon. The representative EAEC O44 isolates carried bla CTX-M-14 on the chromosome, which was surrounded by the IS Ecp1 - bla CTX-M-14 - IS903 transposon. To the best of our knowledge, this is the first report of co-infection with chromosomally located bla CTX-M-14 and plasmid-encoding bla CTX-M-15 in pathogenic E. coli . Our findings indicate that resistance genes in clinical isolates can spread through concurrent combinations of chromosomes and plasmids.

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“…Downstream to bla CTX–M– 15 we identified a 169 bp fragment of bla TEM– 1 B , the passenger gene of Tn 2 ( Siguier et al, 2006 ; Bailey et al, 2011 ). This bla CTX–M– 15 context, including the bla TEM– 1 B fragment, has only been described once in the literature and was present in ETEC O159:H20 strains responsible for the first ESBL-associated foodborne outbreak in South Korea in 2016 ( Kim et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Multidrug-Resistant Lineage of Enterotoxigenic Escherichia coli ST182 With Serotype O169:H41 in Airline Waste

et al. 2021

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Enterotoxigenic Escherichia coli (ETEC) is the primary aetiologic agent of traveller’s diarrhoea and a significant cause of diarrhoeal disease and death in developing countries. ETEC O169:H41 strains are known to cause both traveller’s diarrhoea and foodborne outbreaks in developed countries and are cause for concern. Here, whole-genome sequencing (WGS) was used to assemble 46 O169:H41 (ST182) E. coli draft genomes derived from two airplane waste samples sourced from a German international airport. The ST182 genomes were compared with all 84 publicly available, geographically diverse ST182 genomes to construct a core genome-based phylogenetic tree. ST182 isolates were all phylogroup E, the majority serotype O169:H41 (n = 121, 93%) and formed five major clades. The airplane waste isolates differed by an average of 15 core SNPs (range 0–45) but their accessory genome content was diverse. While uncommon in other ST182 genomes, all airplane-derived ST182 isolates carried: (i) extended-spectrum β-lactamase gene blaCTX–M–15 notably lacking the typical adjacent ISEcp1; (ii) qnrS1 and the S83L mutation in gyrA, both conferring resistance to fluoroquinolones; and (iii) a class 1 integron structure (IS26-intI1Δ648-dfrA17-aadA5-qacEΔ1-sul1-ORF-srpC-padR-IS6100-mphR-mrx-mphA-IS26) identified previously in major extraintestinal pathogenic E. coli STs but not in ETEC. ST182 isolates carried ETEC-specific virulence factors STp + CS6. Adhesin/invasin tia was identified in 89% of aircraft ST182 isolates (vs 23%) and was located on a putative genomic island within a hotspot region for various insertions including PAI I536 and plasmid-associated transposons. The most common plasmid replicons in this collection were IncFII (100%; F2:A-:B-) and IncB/O/K/Z (89%). Our data suggest that potentially through travel, E. coli ST182 are evolving a multidrug-resistant profile through the acquisition of class 1 integrons and different plasmids.

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Outbreak of CTX-M-15-Producing Enterotoxigenic Escherichia coli O159:H20 in the Republic of Korea in 2016

Cited by 9 publications

References 17 publications

Antimicrobial-Resistant Escherichia coli Strains and Their Plasmids in People, Poultry, and Chicken Meat in Laos

Antimicrobial-Resistant Escherichia coli Strains and Their Plasmids in People, Poultry, and Chicken Meat in Laos

Co-infection With Chromosomally-Located blaCTX-M-14 and Plasmid-Encoding blaCTX-M-15 in Pathogenic Escherichia coli in the Republic of Korea

Multidrug-Resistant Lineage of Enterotoxigenic Escherichia coli ST182 With Serotype O169:H41 in Airline Waste

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