Outbreaks of vibriosis associated with Vibrio mimicus in freshwater catfish in China

Geng, Yi; Liu, D.; Han, Shunshun; Zhou, Yan; Wang, K.Y.; Huang, Xiaoli; Chen, D.F.; Peng, Xi; Lai, Weiming

doi:10.1016/j.aquaculture.2014.05.053

Cited by 70 publications

(41 citation statements)

References 14 publications

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“…It is a foodborne pathogen [2], which can cause gastroenteritis, diarrhea and food poisoning [3][4][5]. V. mimicus infecting was also common in aquaculture (can infect shrimp, crab, and fish) [6][7][8][9][10][11]. In recent years, V. mimicus is an emerging pathogen in some species of Siluriformes.…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, V. mimicus is an emerging pathogen in some species of Siluriformes. The epidemiological features of V. mimicus showed short disease duration and high mortality rate, which eventually leads to substantial economic loss in Siluriformes farmhouses [9,10]. Since 2011, large-scale V. mimicus infectious outbreaks have occurred continuously in Siluriformes farms in China.…”

Section: Introductionmentioning

confidence: 99%

“…Since 2011, large-scale V. mimicus infectious outbreaks have occurred continuously in Siluriformes farms in China. V. mimicus strain SCCF01, which is a highly virulent strain isolated from yellow catfish (Pelteobagrus fulvidraco) in China, causes almost 100% mortality in yellow catfish [9,12].…”

Section: Introductionmentioning

confidence: 99%

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Complete genome analysis of Vibrio mimicus strain SCCF01, a highly virulent isolate from the freshwater catfish

Wang

Geng

et al. 2019

Virulence

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2020)Complete genome analysis of Vibriomimicus strain SCCF01, a highly virulent isolate from the freshwater catfish, Virulence, 11:1, 23-31, ABSTRACT Vibrio mimicus is a foodborne pathogen, which is widely distributed in the aquatic environment. Moreover, it is often involved in aquatic animal diseases. In recent years, V. mimicus is an emerging pathogen in some species of Siluriformes. The strain SCCF01 was isolated from yellow catfish (Pelteobagrus fulvidraco). In this study, we aimed to perform genomic analysis of V. mimicus strain SCCF01 to identify genetic features and evolutionary relationships. Information on gene function and classification was obtained by functional annotation, and circular graph of strain SCCF01 genome, which was created by Circos v0.64. Information on virulence genes (adhesion, flagellum system, exotoxin, and secretory system, etc.) was obtained by virulence genes annotation. Genome element prediction showed that most of the mobile elements were distributed in chromosome I. Therefore, chromosome I of SCCF01 genome has more plasticity than chromosome II and might be larger in size. Genomic linear relationship between the strain of V. mimicus and strain SCCF01 was analyzed by linear pairwise comparison but was unable to determine the relationship. Gene family analysis predicted that the evolutionary direction of strain SCCF01 was: clinical strain → environmental strain → SCCF01 strain. Phylogenetic analysis showed that the strain SCCF01 was more closely related to environmental strains. According to gene family analysis and phylogenetic analysis, we speculated that strain SCCF01 has probably diverged from environmental strains. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Complete genome analysis of Vibrio mimicus strain SCCF01, a highly virulent isolate from the freshwater catfish

Wang

Geng

et al. 2019

Virulence

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“…Environmental pollution and water quality deterioration in the aquatic environment might be incriminated as primary stress factors that promoted the invasion of secondary bacteria and yeast, such as Pseudomonas fluorescens and Candida albicans (Eissa et al 2013), and multiple zoonotic parasites (as reviewed by Mahmoud et al 2014). Several infectious diseases, such as streptococcosis, vibriosis, photobacteriosis, branchiomycosis, saprolegniasis, and many others, have been reported in disastrous cases of mass mortalities in cultured and wild fish populations worldwide (Al-Harbi 1994;Perera et al 1994;Paperna and Smirnova 1997;Yuasa et al 1999;Glibert et al 2002;Hussein and Hatai 2002;Gaikowski et al 2003;Villamil et al 2003;Zorrilla et al 2003;Mohamed et al 2004;Evans et al 2006b;Ebrahim 2011;Geng et al 2014;Abu-Elala et al 2015).…”

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confidence: 99%

Eutrophication, Ammonia Intoxication, and Infectious Diseases: Interdisciplinary Factors of Mass Mortalities in Cultured Nile Tilapia

et al. 2016

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The present study was designed to assess the possible causes of the mass mortalities of Nile Tilapia Oreochromis niloticus at El-Behera Governorate, Egypt, in relationship to environmental and microbiotic factors. Water samples were collected from fish farms at different locations and from Lake Edku to analyze water temperature, water pH, salinity, biological oxygen demand, dissolved oxygen, total ammonia nitrogen, and un-ionized ammonia. A number of moribund and freshly dead fish were sampled and submitted to our laboratory for microbiological, molecular, and histopathological examination. Water analysis of the fish farms revealed noticeable increases in the previously mentioned physicochemical parameters. Clinical examinations of moribund fish showed severe gill rot and massive external and internal hemorrhages. Ordinary and molecular laboratory findings confirmed the presence of Branchiomyces sp. in gill tissue and mixed bacterial fish pathogens (Streptococcus agalactiae, Vibrio alginolyticus, V. parahaemolyticus, Pseudomonas anguilliseptica, and P. aeruginosa) in visceral organs. The histopathological and transmission electron microscopic examinations revealed severe necrosis of gill filaments and blockage of branchial blood vessels and lamellar capillaries with Branchiomyces sp. hyphae and spores mixed with different shapes of bacteria. Severe inflammations were detected in liver, kidney, heart, and brain tissues. Ultimately, we can conclude that the syndrome of mass fish kills in this area is a consequence of ecological damage to the aquatic environment, which is mainly related to natural and anthropogenic factors, as well as to the presence of infectious agents. Received September 30, 2015; accepted April 12, 2016.

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“…mimicus ) is a common pathogen found in aquaculture that can cause severe ascites disease in fish [1–2]. The controlling methods for ascites disease in fish at present are still mainly oral antibiotics and topical chemical disinfectants.…”

Section: Introductionmentioning

confidence: 99%

Screening a Phage Display Library for Two Novel OmpU-Binding Peptides with Adhesion Antagonistic Activity against Vibrio mimicus

et al. 2016

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Vibrio mimicus is a pathogen that causes ascites disease in fish. We have previously demonstrated that the outer membrane protein U (OmpU) is an important adhesin in V. mimicus. Here eight specific OmpU-binding phage clones, which presented three different OmpU-binding peptides (designated P1, P2, P3), were screened from a commercially available phage displayed 12-mer peptide library using rOmpU protein as target. Then, synthetic OmpU-binding peptides were measured for their adhesion antagonistic activity and binding affinity via adhesion inhibition test and non-competitive ELISA, respectively. The results showed that after co-incubated with the mixture of rOmpU and P3, visible green fluorescence could be observed on the epithelioma papulosum cyprinidi (EPC) cells surface; while the EPC cells co-incubated with the mixture of rOmpU and P1/P2 exhibited little green fluorescence. The average adhesion number of V. mimicus 04–14 isolate before and after treatment with peptide was 21.4 ± 1.5, 20.8 ± 0.8 (irrelevant peptide), 20.2 ± 0.5 (P3), 5.1 ± 0.7 (P1) and 3.4 ± 0.8 (P2), respectively. There was a significant decrease in the adhesive level of 04–14 isolate treated with P1/ P2 compared to the untreated isolate (p<0.01). The affinity constants of P1 and P2 were (6.17 ± 0.19) × 108 L/mol and (1.24 ± 0.56) × 109 L/mol, respectively. Furthermore, protective effects of P1 and P2 on grass carps challenged with V. mimicus were preliminary detected. It was found there was delayed death of fish in the groups treated with P1/P2, and the survival rate of challenged fish improved with the increase of the dose of adhesion antagonistic peptide. Taken together, two novel OmpU-binding peptides, which possessed adhesion antagonistic activity, high affinity and a certain degree of antibacterial activity against V. mimicus, were screened and identified.

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Outbreaks of vibriosis associated with Vibrio mimicus in freshwater catfish in China

Cited by 70 publications

References 14 publications

Complete genome analysis of Vibrio mimicus strain SCCF01, a highly virulent isolate from the freshwater catfish

Complete genome analysis of Vibrio mimicus strain SCCF01, a highly virulent isolate from the freshwater catfish

Eutrophication, Ammonia Intoxication, and Infectious Diseases: Interdisciplinary Factors of Mass Mortalities in Cultured Nile Tilapia

Screening a Phage Display Library for Two Novel OmpU-Binding Peptides with Adhesion Antagonistic Activity against Vibrio mimicus

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