2021
DOI: 10.1002/ar.24689
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Outer and inner mitochondrial membrane proteins TOMM40 and TIMM50 are intensively concentrated and localized at Purkinje and pyramidal neurons in the New Zealand white rabbit brain

Abstract: Mitochondria are involved in a variety of developmental processes and neurodegenerative diseases. The translocase complexes of the outer and inner mitochondrial membranes (TOM and TIM) are protein complexes involved in transporting protein precursors across mitochondrial membranes. Although rabbits are important animal models for neurodegenerative diseases, the expression of TOM and TIM complexes has yet to be examined in the rabbit brain. In the present study, we quantitatively evaluated the protein expressio… Show more

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Cited by 4 publications
(3 citation statements)
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“…Paraffin sections of 4 μm in thickness representing all parts of the digestive tract of the moorhen were prepared for DAB (3, 3'-diaminobenzidine) immunohistochemistry as previously described [ 29 , 30 ]. Briefly, sections were dewaxed in xylene, washed in phosphate-buffered saline (PBS), and boiled in sodium citrate (pH = 6) inside a microwave for 20 min.…”
Section: Methodsmentioning
confidence: 99%
“…Paraffin sections of 4 μm in thickness representing all parts of the digestive tract of the moorhen were prepared for DAB (3, 3'-diaminobenzidine) immunohistochemistry as previously described [ 29 , 30 ]. Briefly, sections were dewaxed in xylene, washed in phosphate-buffered saline (PBS), and boiled in sodium citrate (pH = 6) inside a microwave for 20 min.…”
Section: Methodsmentioning
confidence: 99%
“…The immunohistochemical study was performed on dewaxed paraffin sections as previously stated (Abdellatif, 2021a(Abdellatif, , 2021bFarouk et al, 2022). Briefly, the antigenic sites were recovered via heating the sections in citrate buffer (pH = 6).…”
Section: Histological and Immunohistochemical Analysesmentioning
confidence: 99%
“…Following their dewaxing and rehydration, tissue sections selected for the immunohistochemical procedure were prepared as previously described (15,16). Briefly, antigenic epitopes were retrieved via boiling the sections in citrate buffer (pH = 6) using a microwave at 750 watts for 20 minutes.…”
Section: Immunohistochemistrymentioning
confidence: 99%