Outer membrane protein K as a subunit vaccine against V. anguillarum

Current growth in aquaculture production is parallel with the increasing number of disease outbreaks, which negatively affect the production, profitability, and sustainability of the global aquaculture industry. Vibriosis is among the most common diseases leading to massive mortality of cultured shrimp, fish, and shellfish in Asia. High incidence of vibriosis can occur in hatchery and grow-out facilities, but juveniles are more susceptible to the disease. Various factors, particularly the source of fish, environmental factors (including water quality and farm management), and the virulence factors of Vibrio, influence the occurrence of the disease . Affected fish show weariness, with necrosis of skin and appendages, leading to body malformation, slow growth, internal organ liquefaction, blindness, muscle opacity, and mortality. A combination of control measures, particularly a disease-free source of fish, biosecurity of the farm, improved water quality, and other preventive measures (e.g., vaccination) might be able to control the infection. Although some control measures are expensive and less practical, vaccination is effective, relatively cheap, and easily implemented. In this review, the latest knowledge on the pathogenesis and control of vibriosis, including vaccination, is discussed. 4INA-SALWANY ET AL.

show abstract

“…; Hamod et al. ; Nehlah et al. ), anti‐idiotypic (Qin and Yan ), and toxoid vaccines (Sudhagar et al.…”

Section: Potential Control and Prevention Measures Against Vibriosismentioning

confidence: 99%

Vibriosis in Fish: A Review on Disease Development and Prevention

et al. 2018

View full text Add to dashboard Cite

show abstract

“…OMP (A. hydrophila, V. harveyi, Stenotrophomonas maltophilia & V.alginolyticus) were tested in aquaculture animals such as rohu (Yadav et al, 2018) , channel catfish (Abdelhamed et al, 2017;Wang et al, 2016) , goldfish (Carassius auratus) (Divya et al, 2015) , turbot (Scophthalmus maximus) (Wang et al, 2011) and tiger shrimp (Maftuch et al, 2013) showed stimulation towards immune responses (up-regulation of immune-related genes, non-specific parameters and specific antibodies) , reduced bacterial load and mortality. Recombinant OMPs (Flavobacterium columnare, V. anguillarum, Piscirickettisia salmonis & A. hydrophila) were successfully synthesized and tested in grass carp (Luo et al, 2016) , rohu (Hamod et al, 2012) , coho salmon (Oncorhynchus kisutch) (Kuzyk et al, 2001) and common carp (Maiti et al, 2012) . Development of polyvalent vaccine with OMPs from A. hydrophila (Ni et al, 2010) , V. parahaemolyticus (Li et al, 2010) and V. alginolyticus (Nehlah et al, 2016) has been successful.…”

Section: Cell Surface Proteinsmentioning

confidence: 99%

Postbiotics Applications as Infectious Disease Control Agent in Aquaculture

et al. 2020

View full text Add to dashboard Cite

Aquaculture is developing so fast that infectious disease outbreak happens regularly. Antibiotic treatment results in development of antibiotic resistance pathogens, thus cause urgent action in searching of other alternative treatment method. Postbiotic was one of the explored strategies among various proposed alternatives. Due to its benefits in agriculture industry, it may be useful in aquaculture industry. Although many reviews were reported on other alternative strategies, the review on postbiotic in aquaculture is limited. This mini review provides an overview of different postbiotics as aquaculture disease control agents. Peptides and exopolysaccharides have antimicrobial properties against bacterial pathogens. Then, short chain fatty acids have both antimicrobial activities against bacterial pathogens and immunostimulating effects to aquatic organism. Vitamins, peptidoglycan and lipopolysaccharide are reported as immunostimulants. Finally, cell surface proteins and teichoic acid can act as vaccine.

show abstract

“…Immunogenicity of porins is attributed to various mechanisms such as inhibition of phagocytosis by activating the adenylate cyclase system [26], production of vast array of cytokines [27] and activating the complement system of host [28]. In agreement to this, OMPs from different bacterial strains such as outer membrane protein F (OprF) and outer membrane protein I (OprI) of P. aeruginosa [29], and OmpK of V. anguillarum [30] and Vibrio harveyi [31], have been reported to be highly immunogenic and capable of conferring protection against challenge with the corresponding bacteria.…”

Section: Discussionmentioning

confidence: 98%

Recombinant outer membrane protein C of Aeromonas hydrophila elicits mixed immune response and generates agglutinating antibodies

et al. 2016

View full text Add to dashboard Cite

Aeromonas hydrophila is a gram-negative fish pathogenic bacterium, also responsible for causing opportunistic pathological conditions in humans. It causes a number of diseases in fish due to which the fish industry incurs huge economic losses annually. Due to problems of antibiotic resistance, and the rapidity with which the infection spreads among fishes, vaccination remains the most effective strategy to combat this infection in fish populations. Among various virulence factors associated with bacterial virulence, outer membrane proteins have been widely evaluated for their vaccine potential owing to their surface exposure and related role in pathogenicity. In the present study, we have investigated the immunogenic potential of a non-specific porin, outer membrane protein C (OmpC) whose expression is regulated by the two-component regulatory system and plays a major role in the survival of A. hydrophila under different osmolaric conditions. The full-length gene (~1 kb) encoding OmpC of A. hydrophila was cloned, characterized and expressed in E. coli. High yield (~112 mg/L at shake flask level) of the recombinant OmpC (rOmpC) (~40 kDa) of A. hydrophila was obtained upon purification from inclusion bodies using Ni(2+)-NTA affinity chromatography. Immunization with purified rOmpC in murine model generated high endpoint (>1:40,000) titers. IgG isotyping, ELISA and ELISPOT assay indicated mixed immune response with a TH2 bias. Also, the anti-rOmpC antibodies were able to agglutinate A. hydrophila in vitro and exhibited specific cross-reactivity with different Aeromonas strains, which will facilitate easy detection of different Aeromonas isolates in infected samples. Taken together, these data clearly indicate that rOmpC could serve as an effective vaccine against different strains of Aeromonas, a highly heterogenous group of bacteria.

show abstract

Outer membrane protein K as a subunit vaccine against V. anguillarum

Cited by 40 publications

References 16 publications

Vibriosis in Fish: A Review on Disease Development and Prevention

Vibriosis in Fish: A Review on Disease Development and Prevention

Postbiotics Applications as Infectious Disease Control Agent in Aquaculture

Recombinant outer membrane protein C of Aeromonas hydrophila elicits mixed immune response and generates agglutinating antibodies

Contact Info

Product

Resources

About