Outer Membrane Protein STM3031 (Ail/OmpX-Like Protein) Plays a Key Role in the Ceftriaxone Resistance of
            <i>Salmonella enterica</i>
            Serovar Typhimurium

Hu, Wen; Lin, Jing-Fang; Lin, Ying-Hsiu; Chang, Hsin-Yu

doi:10.1128/aac.00079-09

Cited by 26 publications

(25 citation statements)

References 28 publications

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“…S. enterica serovar Typhimurium strain, R200(⌬stm3031) was generated from strain R200. All these strains have been described previously (18). Strains R200(⌬stm1530), R200(⌬cpxAR), and R200(⌬baeSR) were generated from strain R200.…”

Section: Methodsmentioning

confidence: 99%

“…Outer membrane fraction preparation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for protein analysis were performed as described previously (17,18). The detection of STM3031 protein or STM1530 protein was by Western blotting as described previously (18).…”

Section: Methodsmentioning

confidence: 99%

“…As part of this efflux pump, TolC is an outer membrane protein and has been suggested to cooperate with a number of transporters, such as AcrD and MdtABC, to expel drugs from bacteria (2,24,26). In a previous study, we showed that significant increases in the levels of outer membrane protein STM3031 and putative outer membrane protein STM1530 were present in the ceftriaxone-resistant strain R200 (18). Of these proteins, STM3031 has been suggested to play a key role in ceftriaxone resistance, possibly by reducing permeability via a decreased OmpD level and by enhancing export via an increase in AcrD efflux pump activity (18).…”

mentioning

confidence: 99%

“…In a previous study, we showed that significant increases in the levels of outer membrane protein STM3031 and putative outer membrane protein STM1530 were present in the ceftriaxone-resistant strain R200 (18). Of these proteins, STM3031 has been suggested to play a key role in ceftriaxone resistance, possibly by reducing permeability via a decreased OmpD level and by enhancing export via an increase in AcrD efflux pump activity (18). Furthermore, the expression of this protein is influenced by baeR, the regulator gene of a two-component system of S. enterica serovar Typhimurium (17).…”

mentioning

confidence: 99%

“…Using the various Salmonella strains, the separation of the bacterial cells into cytoplasmic, periplasmic, inner membrane, and outer membrane fractions was carried out as previously described (18).…”

mentioning

confidence: 99%

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The Expression Levels of Outer Membrane Proteins STM1530 and OmpD, Which Are Influenced by the CpxAR and BaeSR Two-Component Systems, Play Important Roles in the Ceftriaxone Resistance of Salmonella enterica Serovar Typhimurium

Hu¹,

Chen²,

Zhang³

et al. 2011

Antimicrob Agents Chemother

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Significant increases in STM3031, STM1530, and AcrD protein levels and significant decreases in OmpC and OmpD protein levels are present when the ceftriaxone-resistant Salmonella enterica serovar Typhimurium R200 strain is compared with the ceftriaxone-susceptible strain 01-4. AcrD is known to be involved in drug export, and STM3031 seems to play a key role in ceftriaxone resistance. Here, we examine the roles of STM1530, OmpC, and OmpD in ceftriaxone resistance. An ompD gene deletion mutant showed 4-fold higher ceftriaxone resistance than 01-4. An ompC gene deletion mutant showed 4-fold higher cephalothin and erythromycin resistance than 01-4, but there was no effect on ceftriaxone resistance. However, a stm1530 deletion mutant did show >64-fold lower ceftriaxone resistance than R200. Moreover, the STM3031 protein was significantly decreased in R200(⌬stm1530) compared to R200. STM3031 expression has been shown to be influenced by the two-component system regulator gene baeR. CpxR seems to modulate BaeR. A cpxA-cpxR gene deletion mutant showed >2,048-fold lower ceftriaxone resistance than R200. The outer membrane protein profile of R200(⌬cpxAR) showed significant decreases in STM3031 and STM1530 compared to R200, while OmpD had returned to the level found in 01-4. Furthermore, the stm3031, stm1530, and ompD mRNA levels were correlated with their protein expression levels in these strains, while decreases in the mRNA levels of the efflux pump acrB, acrD, and acrF genes were found in R200(⌬cpxAR). Findings similar to those for R200(⌬cpxAR) were found for R200(⌬baeSR). These results, together with those for STM3031 and the fact that STM1530 is an outer membrane protein, suggest that STM1530 and OmpD are influenced by the CpxAR and BaeSR two-component systems and that this contributes to S. enterica serovar Typhimurium ceftriaxone resistance.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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The Expression Levels of Outer Membrane Proteins STM1530 and OmpD, Which Are Influenced by the CpxAR and BaeSR Two-Component Systems, Play Important Roles in the Ceftriaxone Resistance of Salmonella enterica Serovar Typhimurium

Hu¹,

Chen²,

Zhang³

et al. 2011

Antimicrob Agents Chemother

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Chromosomally encoded and plasmid-mediated polymyxins resistance in Acinetobacter baumannii: a huge public health threat

Lima

Alves

Cruz

et al. 2018

Eur J Clin Microbiol Infect Dis

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Acinetobacter baumannii is an opportunistic pathogen associated with nosocomial and community infections of great clinical relevance. Its ability to rapidly develop resistance to antimicrobials, especially carbapenems, has re-boosted the prescription and use of polymyxins. However, the emergence of strains resistant to these antimicrobials is becoming a critical issue in several regions of the world because very few of currently available antibiotics are effective in these cases. This review summarizes the most up-to-date knowledge about chromosomally encoded and plasmid-mediated polymyxins resistance in A. baumannii. Different mechanisms are employed by A. baumannii to overcome the antibacterial effects of polymyxins. Modification of the outer membrane through phosphoethanolamine addition, loss of lipopolysaccharide, symmetric rupture, metabolic changes affecting osmoprotective amino acids, and overexpression of efflux pumps are involved in this process. Several genetic elements modulate these mechanisms, but only three of them have been described so far in A. baumannii clinical isolates such as mutations in pmrCAB, lpxACD, and lpsB. Elucidation of genotypic profiles and resistance mechanisms are necessary for control and fight against resistance to polymyxins in A. baumannii, thereby protecting this class for future treatment.

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Molecular identification and differential proteomics of drug resistant Salmonella Typhi

Safi

Bendixen

Rahman

et al. 2023

Diagnostic Microbiology and Infectious Disease

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Outer Membrane Protein STM3031 (Ail/OmpX-Like Protein) Plays a Key Role in the Ceftriaxone Resistance of Salmonella enterica Serovar Typhimurium

Cited by 26 publications

References 28 publications

The Expression Levels of Outer Membrane Proteins STM1530 and OmpD, Which Are Influenced by the CpxAR and BaeSR Two-Component Systems, Play Important Roles in the Ceftriaxone Resistance of Salmonella enterica Serovar Typhimurium

The Expression Levels of Outer Membrane Proteins STM1530 and OmpD, Which Are Influenced by the CpxAR and BaeSR Two-Component Systems, Play Important Roles in the Ceftriaxone Resistance of Salmonella enterica Serovar Typhimurium

Chromosomally encoded and plasmid-mediated polymyxins resistance in Acinetobacter baumannii: a huge public health threat

Molecular identification and differential proteomics of drug resistant Salmonella Typhi

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