“…In recent years, integration of different techniques is gradually taking up multi-dimensional shape in developing new methodological approaches to deal with complex biological systems. Cryo-EM, having received a great attention nowadays for determining 3D structures of intrinsically dynamic macromolecular assemblies ( Lawson et al., 2020 ; Chen and He, 2020 ), mostly populates new integrative approaches ( Figure 1 ). One such integrative structural biology technique is the correlative light electron microscopy method (CLEM), where the tomography approach of cryo-EM is combined with light microscopy ( Koning et al., 2018 ).…”