Ovarian activity and oocyte quality associated with the biochemical profile of serum and follicular fluid from Girolando dairy cows postpartum

Alves, Bênner Geraldo; Alves, Kele Amaral; Lucio, A. C.; Martins, Muller Carrara; Silva, Thiago H.; Braga, Lucas S.; Silva, Thiago V.; Viu, M. A. O.; Beletti, Marcelo Emı́lio; Jacomini, José Octávio; Santos, Ricarda Maria dos; Gambarini, M. L.

doi:10.1016/j.anireprosci.2014.02.019

Cited by 34 publications

(31 citation statements)

References 42 publications

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“…Similarly to this work, the concentration of sodium in the serum (or blood's plasma) was higher in the ovarian follicles fl uid in cows and buffalos, as noted in other works [6,7,22]. However, it was determined that sodium concentration higher in cystic fl uid than in the pre-ovulatory follicles fl uid might be caused by disorders of follicular fl uid production.…”

Section: Discussionsupporting

confidence: 82%

“…In ovarian follicles it also participates in processes connected with meiotic maturation of oocytes, in gonadotropin regulation of steroidogenesis and ovulation, as well as in atresia of follicles [1,28]. On the other hand, it has been proven that there is a positive correlation between calcium concentration in the ovarian fl uid and the number of degenerated oocytes in dairy cows [22]. It cannot be ruled out that the concentration of calcium higher in the cystic fl uid than in pre-ovulatory follicles fl uid presented in this work might be connected with the distorted local regulation of this macroelement during the follicle development.…”

Section: Discussionmentioning

confidence: 99%

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Macro-Elements Composition of Cystic and Follicular Fluid in the Ovaries and their Relationship to Peripheral Blood Concentration in Sows / Odnos Sastava Makroelemenata Folikularne I Cistične Tečnosti Jajnika I Njihove Koncentracije U Perifernoj Krvi Krmača

Stankiewicz

2015

Acta Veterinaria

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The aim of this work is the comparison of sodium, calcium, magnesium and phosphorus concentration in pre-ovulatory follicles and follicular cysts and their concentration in the serum of sows. The research was conducted on sows (Polish Large White x Polish Landrace crossbred). Sodium concentration in cystic fl uid was higher than in the follicular fl uid (P<0.05). Sodium concentration in the serum of cysts-bearing sows was higher than in non-cysts-bearing sows (P<0.01). Differences were also observed between cysts and serum of cysts-bearing sows (P<0.01), as well as follicles and serum of non-cysts-bearing sows (P<0.01). Calcium concentration in the serum of non-cystsbearing sows was higher than in the case of cysts-bearing sows (P<0.01). Differences were also between calcium concentration in the cysts and its concentration in the serum of cysts-bearing sows (P<0.05). Magnesium concentration in the serum of cysts-bearing sows was lower than in non-cysts-bearing sows (P<0.01). Differences in magnesium concentration were also between cysts and serum of cysts-bearing sows (P<0.01), as well as between follicles and serum of non-cysts-bearing sows (P<0.05). Phosphorus concentration in the cysts was lower than in the follicles (P<0.05). It was also lower in the serum of non-cysts-bearing sows than in the follicles (P<0.05). The concentration of sodium, magnesium and phosphorus in the serum was positively correlated with the concentration of the mentioned macro-elements in ovarian structures (r = 0.66, P<0.01; r = 0.49, and r = 0.48, P<0.05, respectively). A negative correlation was found between the concentration of serum calcium and the ovarian structures (r = -0.47, P<0.05).The differences in the concentrations of macro-elements suggest that the ionic composition may be one of the factors associated with the processes occurring in the ovarian structures of pigs.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Macro-Elements Composition of Cystic and Follicular Fluid in the Ovaries and their Relationship to Peripheral Blood Concentration in Sows / Odnos Sastava Makroelemenata Folikularne I Cistične Tečnosti Jajnika I Njihove Koncentracije U Perifernoj Krvi Krmača

Stankiewicz

2015

Acta Veterinaria

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“…Summer heat stress (HS) is a major factor in decreased reproductive performance in dairy cattle, possibly by affecting the metabolism capacity of ovarian follicles (Alves et al 2014;Roth 2008). The current study offers the first thorough insight into the transcriptome analysis of primary bovine GCs during heat stress using DGE technology.…”

Section: Discussionmentioning

confidence: 99%

“…Biological process analyzed (c) of regulated genes (up and down genes) that changed fold ≥2 folds by Gene KEGG analysis (Rispoli et al 2013), and thermal stress could induced sheep granulosa cell apoptosis (Fu et al 2014). Heat stress could induce metabolic changes, which compromise the number of ovarian follicles and the follicular environment, thus resulting in morphologically damaged oocytes (Alves et al 2014). In the ovary, GCs are responsible for establishing the follicular microenvironment; any factors that impair GCs may disrupt oocytes (Guerrero-Netro et al 2015;Rawan et al 2015).…”

Section: Discussionmentioning

confidence: 99%

The effect of heat stress on gene expression, synthesis of steroids, and apoptosis in bovine granulosa cells

Luo

et al. 2016

Cell Stress and Chaperones

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Summer heat stress (HS) is a major contributing factor in low fertility in lactating dairy cows in hot environments. Heat stress inhibits ovarian follicular development leading to diminished reproductive efficiency of dairy cows during summer. Ovarian follicle development is a complex process. During follicle development, granulosa cells (GCs) replicate, secrete hormones, and support the growth of the oocyte. To obtain an overview of the effects of heat stress on GCs, digital gene expression profiling was employed to screen and identify differentially expressed genes (DEGs; false discovery rate (FDR) ≤ 0.001, fold change ≥2) of cultured GCs during heat stress. A total of 1211 DEGs including 175 upregulated and 1036 downregulated ones were identified, of which DEGs can be classified into Gene Ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The results suggested that heat stress triggers a dramatic and complex program of altered gene expression in GCs. We hypothesized that heat stress could induce the apoptosis and dysfunction of GCs. Real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to evaluate the expression of steroidogenic genes (steroidogenic acute regulatory protein (Star), cytochrome P-450 (CYP11A1), CYP19A1, and steroidogenic factor 1 (SF-1)) and apoptosis-related genes (caspase-3, BCL-2, and BAX). Radio immunoassay (RIA) was used to analyze the level of 17β-estradiol (E 2 ) and progesterone (P 4 ). We also assessed the apoptosis of GCs by flow cytometry. Our data suggested that heat stress induced GC apoptosis through the BAX/BCL-2 pathway and reduced the steroidogenic gene messenger RNA (mRNA) expression and E 2 synthesis. These results suggest that the decreased function of GCs may cause ovarian dysfunction and offer an improved understanding of the molecular mechanism responsible for the low fertility in cattle in summer.

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“…; Alves et al . ). However, no difference was observed between Hol and JB in the maturation rate after IVM when only oocytes with good morphology (GI or GII) were used for the IVM.…”

Section: Discussionmentioning

confidence: 97%

Different thermotolerances in in vitro‐produced embryos derived from different maternal and paternal genetic backgrounds

Sakatani

Yamanaka

Balboula

et al. 2017

Animal Science Journal

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The present study evaluated the effects of genetic backgrounds on the developmental competence and thermotolerance of bovine in vitro-produced (IVP) embryos. First, Holstein (Hol) and Japanese Black (JB) oocytes were fertilized with sperm from Hol, JB and a thermotolerant breed (Brahman), and in vitro development was evaluated when the embryos were exposed to heat shock on Day 2 (Day 0 = day of fertilization). Sperm genetic backgrounds affected the developmental competence in controls (P < 0.05). Second, the effect of sperm pre-incubation for 4 h on subsequent in vitro fertilization was assessed using different sperm genetic backgrounds. The pre-incubation of sperm did not decrease the embryonic development regardless of the breed of the sperm. A milder heat shock (40.0°C) effect on parthenotes (Hol and JB) and IVP embryos were evaluated. JB parthenotes showed developmental arrest after Day 4, and the rate of development to the blastocyst stage decreased by heat shock, but not in Hol parthenotes. Heat shock decreased developmental competence after cleavage of IVP embryos regardless of genetic background. The thermotolerance of IVP embryos would be controlled by both maternal and paternal factors but genetic involvement was still unclear. Further evaluation is needed to reveal the genetic contribution to thermotolerance.

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Ovarian activity and oocyte quality associated with the biochemical profile of serum and follicular fluid from Girolando dairy cows postpartum

Cited by 34 publications

References 42 publications

Macro-Elements Composition of Cystic and Follicular Fluid in the Ovaries and their Relationship to Peripheral Blood Concentration in Sows / Odnos Sastava Makroelemenata Folikularne I Cistične Tečnosti Jajnika I Njihove Koncentracije U Perifernoj Krvi Krmača

Macro-Elements Composition of Cystic and Follicular Fluid in the Ovaries and their Relationship to Peripheral Blood Concentration in Sows / Odnos Sastava Makroelemenata Folikularne I Cistične Tečnosti Jajnika I Njihove Koncentracije U Perifernoj Krvi Krmača

The effect of heat stress on gene expression, synthesis of steroids, and apoptosis in bovine granulosa cells

Different thermotolerances in in vitro‐produced embryos derived from different maternal and paternal genetic backgrounds

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