Ovarian contrast-enhanced ultrasonography and Doppler fluxometry in bitches during the postovulatory estrus and corpora lutea formation

Aires, Luiz Paulo Nogueira; Gasser, Beatriz; Silva, Priscila; Silva, Priscila Del Aguila da; Yamada, Diego Iwao; Carneiro, Rafael Kretzer; Lima, Bruna Bruna Bressianini; Padilha-Nakaghi, Luciana Cristina; Uscátegui, Ricardo Andrés Ramírez; Spada, Stefano; Russo, Marco; Feliciano, Marcus Antônio Rossi

doi:10.1016/j.theriogenology.2022.10.009

Cited by 4 publications

(6 citation statements)

References 39 publications

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“…Results showed that the enhancement, at first minimal during early proestrus, became more evident during the progressing of estrus until the post-ovulatory period. PPI, AUC, and MTT were significantly higher in the last evaluation, while TTP and pixels remained unchanged during all evaluations [82].…”

Section: Ceus and Ovariesmentioning

confidence: 85%

“…In conclusion, CEUS can help monitor the increase in vascularization during the follicular and post-ovulatory phases of bitches, even if these values are not closely related to increasing progesterone levels [82].…”

Section: Ceus and Ovariesmentioning

confidence: 94%

“…SonoVue ® was injected via IV (0.01 mL/kg), followed by 5 mL of saline solution (NaCl 0.9%). The authors studied the ovarian vascularization pattern during the follicular phase and the early luteal phase, comparing the results to the increasing progesterone levels [82]. The authors verified peak contrast intensity (PPI peak in % of pixels), time to peak (TTP), mean transit time (MTT), area under the curve (AUC), and the average number of pixels.…”

Section: Ceus and Ovariesmentioning

confidence: 97%

“…On the other hand, there is only one recent paper about CEUS and ovaries in veterinary medicine, by Nogueira Aires et al in 2022 [82]. SonoVue ® was injected via IV (0.01 mL/kg), followed by 5 mL of saline solution (NaCl 0.9%).…”

Section: Ceus and Ovariesmentioning

confidence: 99%

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Contrast-Enhanced Ultrasonography (CEUS) in Imaging of the Reproductive System in Dogs: A Literature Review

Sinagra

Orlandi²,

Caspanello

et al. 2023

Animals

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The use of contrast-enhanced ultrasound (CEUS) has been widely reported for reproductive imaging in humans and animals. This review aims to analyze the utility of CEUS in characterizing canine reproductive physiology and pathologies. In September 2022, a search for articles about CEUS in canine testicles, prostate, uterus, placenta, and mammary glands was conducted on PubMed and Scopus from 1990 to 2022, showing 36 total results. CEUS differentiated testicular abnormalities and neoplastic lesions, but it could not characterize tumors. In prostatic diseases, CEUS in dogs was widely studied in animal models for prostatic cancer treatment. In veterinary medicine, this diagnostic tool could distinguish prostatic adenocarcinomas. In ovaries, CEUS differentiated the follicular phases. In CEH-pyometra syndrome, it showed a different enhancement between endometrium and cysts, and highlighted angiogenesis. CEUS was shown to be safe in pregnant dogs and was able to assess normal and abnormal fetal–maternal blood flow and placental dysfunction. In normal mammary glands, CEUS showed vascularization only in diestrus, with differences between mammary glands. CEUS was not specific for neoplastic versus non-neoplastic masses and for benign tumors, except for complex carcinomas and neoplastic vascularization. Works on CEUS showed its usefulness in a wide spectrum of pathologies of this non-invasive, reliable diagnostic procedure.

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Section: Ceus and Ovariesmentioning

confidence: 85%

Section: Ceus and Ovariesmentioning

confidence: 94%

Section: Ceus and Ovariesmentioning

confidence: 97%

Section: Ceus and Ovariesmentioning

confidence: 99%

See 2 more Smart Citations

Contrast-Enhanced Ultrasonography (CEUS) in Imaging of the Reproductive System in Dogs: A Literature Review

Sinagra

Orlandi²,

Caspanello

et al. 2023

Animals

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show abstract

“…Recently, this technique has also found wide application in canine reproduction [ 25 , 26 , 27 ]. The application of CEUS for the evaluation of ovarian activity [ 28 ] and pregnancy [ 29 , 30 ], but also during pyometra [ 31 , 32 ], has been described in the bitch. For male dogs, there are several studies on both healthy [ 33 ] and pathological testes [ 34 , 35 , 36 ]; furthermore, some studies have been published on the prostate in healthy [ 37 , 38 , 39 ], neutered [ 40 ], and pathological animals [ 22 , 37 , 41 , 42 , 43 ].…”

Section: Introductionmentioning

confidence: 99%

Contrast-Enhanced Ultrasound (CEUS) Evaluation of Canine Prostatic Hyperplasia before and after Osaterone Acetate Therapy: Preliminary Results

Pettina,

Bucci,

Mazzetti

et al. 2024

Animals

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The prostate is the only sexual gland of the male dog, and dihydrotestosterone (DHT) regulates its growth. In intact dogs, constant DHT stimulation results in benign prostatic hyperplasia (BPH) that can be treated with osaterone acetate (OSA). This study describes the effects of OSA treatment, detected by contrast-enhanced ultrasonography (CEUS), highlighting prostatic vascularization with a contrast agent composed of gas microbubbles. Fifteen dogs (2–8 years) of different sizes and breeds (4–30 kg) diagnosed with BPH are involved in the study. Before treatment (D0), CPSE is measured (294.05 ± 115.97 ng/mL), and a B-mode ultrasound is performed (Vratio = 2.80 ± 1.85), confirming BPH. CEUS highlights the length of the wash-in (11.93 ± 2.08 s) and wash-out (42.20 ± 6.99 s) phases of the contrast agent in the prostate and the presence of cysts and parenchymal alteration. Dogs are treated with OSA (0.5 mg/kg for 7 days) and reassessed after 21 days (D1): CPSE and prostate volume are significantly (p < 0.001) reduced. The length of the wash-in (14.73 ± 2.54 s) and wash-out (51.13 ± 6.03 s) phases are significantly (p < 0.001) increased. The results confirm the effectiveness of the treatment, particularly the reduction in prostatic perfusion, confirmed by the increase in diffusion times of the contrast. Although preliminary, these findings are promising for the use of CEUS in monitoring dogs with BPH.

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Advantages and difficulties of ultrasound analysis to determine the fertile period in bitches

Holubiev,

Skliarov,

Pérez-Marín

et al. 2023

TAVM

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Definition the fertile period is one of the most important factors contributing to fertilization. Therefore, this analysis is important to solve the problem of infertility. The methods that available today do not provide the expected result, because the vast majority of unsuccessful mating result are related to the incorrect determination of the ovulation period. Therefore, it is important to assess the condition of the ovaries, and the prospect is ultrasound examination, which is an effective method of monitoring the reproductive organs. Ultrasound analysis is a non-invasive and effective method to establish the fertile period in bitches which makes it possible to track changes in the ovaries, detect follicles, their number, and determine the true (morphological) signs of ovulation. Clinicalvisual and hormonal studies which are based on the indirect determination of the optimal period of female insemination have the advantages in compare with vaginal cytology. Particularly, the use of ultrasound in real time allows to visualize the morphological changes of the ovaries and uterus -the irregular shape of the ovaries with large (0.6-1.2 cm) anechoic structures of a rounded or oval shape with a thin capsule, thickening of the endometrium (up to 0.5-0.8 cm) with a hypoechoic structure of the uterine horns. However, in our research we encountered certain difficulties, which turned out to be the same as other authors. Echography of the ovaries can seem a bit complicated, because the follicles that persist in them have a picture similar to that before and after ovulation. Some ovulated follicles do not always completely collapse (fall) during ovulation and are gradually replaced by luteal tissue while maintaining an echogenic picture on the days of the next ovulation. Non-ovulated follicles can complicate sonography interpretation. To diagnose ovulation, an ultrasound analysis should be performed twice a day, which is inconvenient for the owners. But if the ultrasound test is carried out less often than aforementioned number, there is a risk to miss the moment of ovulation, because the follicles before and after ovulation are very similar. An even more significant problem is the location of the ovaries in bitches -costovertebral angle surrounded by fat and an ovarian sac, which can create technical difficulties. In addition, this diagnostic approach may be more difficult in fat or very large dogs, as well as in dogs with thick skin (Shar Pei, Newfoundland).

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Ovarian contrast-enhanced ultrasonography and Doppler fluxometry in bitches during the postovulatory estrus and corpora lutea formation

Cited by 4 publications

References 39 publications

Contrast-Enhanced Ultrasonography (CEUS) in Imaging of the Reproductive System in Dogs: A Literature Review

Contrast-Enhanced Ultrasonography (CEUS) in Imaging of the Reproductive System in Dogs: A Literature Review

Contrast-Enhanced Ultrasound (CEUS) Evaluation of Canine Prostatic Hyperplasia before and after Osaterone Acetate Therapy: Preliminary Results

Advantages and difficulties of ultrasound analysis to determine the fertile period in bitches

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