Ovarian response and embryo gene expression patterns after nonsuperovulatory gonadotropin stimulation in primiparous rabbits does

Álvarez, María Arias; García-García, R.M.; Rebollar, Paloma García; Gutiérrez-Adán, A.; López‐Béjar, Manel; Lorenzo, P.L.

doi:10.1016/j.theriogenology.2012.09.019

Cited by 15 publications

(13 citation statements)

References 52 publications

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“…PLAC8 expression has been reported in many species, such as zebrafish (3), mice (1), bovines (4)(5)(6)(7)(8)(9), and rabbits (16). However, little is known regarding PLAC8 expression in human oocytes and embryos.…”

Section: Discussionmentioning

confidence: 99%

Expression of placenta-specific 8 in human oocytes, embryos, and models of in vitro implantation

Liu

Wang

et al. 2016

Fertility and Sterility

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Section: Discussionmentioning

confidence: 99%

Expression of placenta-specific 8 in human oocytes, embryos, and models of in vitro implantation

Liu

Wang

et al. 2016

Fertility and Sterility

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“…Embryos were washed twice in PBS-PVP solution (1 μg/mL polyvinylpyrrolidone in PBS), snap frozen and stored at -80°C for subsequent RNA extraction. Poly (A) RNA was isolated using the Dynabeads ® mRNA DIRECT ™ Micro Kit (Thermo Fisher Scientific inc., Waltham, Massachusetts, USA) following the manufacturer’s instructions with minor modifications, as previously described [ 45 , 46 ]. Reverse transcription was conducted using the High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific inc.), according to manufacturer’s instructions, and relative levels of each transcript were calculated by normalization to the abundance of β-actin as the internal control.…”

Section: Methodsmentioning

confidence: 99%

Effects of Two Types of Melatonin-Loaded Nanocapsules with Distinct Supramolecular Structures: Polymeric (NC) and Lipid-Core Nanocapsules (LNC) on Bovine Embryo Culture Model

et al. 2016

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Melatonin has been used as a supplement in culture medium to improve the efficiency of in vitro produced mammalian embryos. Through its ability to scavenge toxic oxygen derivatives and regulate cellular mRNA levels for antioxidant enzymes, this molecule has been shown to play a protective role against damage by free radicals, to which in vitro cultured embryos are exposed during early development. In vivo and in vitro studies have been performed showing that the use of nanocapsules as active substances carriers increases stability, bioavailability and biodistribution of drugs, such as melatonin, to the cells and tissues, improving their antioxidant properties. These properties can be modulated through the manipulation of formula composition, especially in relation to the supramolecular structures of the nanocapsule core and the surface area that greatly influences drug release mechanisms in biological environments. This study aimed to evaluate the effects of two types of melatonin-loaded nanocapsules with distinct supramolecular structures, polymeric (NC) and lipid-core (LNC) nanocapsules, on in vitro cultured bovine embryos. Embryonic development, apoptosis, reactive oxygen species (ROS) production, and mRNA levels of genes involved in cell apoptosis, ROS and cell pluripotency were evaluated after supplementation of culture medium with non-encapsulated melatonin (Mel), melatonin-loaded polymeric nanocapsules (Mel-NC) and melatonin-loaded lipid-core nanocapsules (Mel-LNC) at 10−6, 10−9, and 10−12 M drug concentrations. The highest hatching rate was observed in embryos treated with 10−9 M Mel-LNC. When compared to Mel and Mel-NC treatments at the same concentration (10−9 M), Mel-LNC increased embryo cell number, decreased cell apoptosis and ROS levels, down-regulated mRNA levels of BAX, CASP3, and SHC1 genes, and up-regulated mRNA levels of CAT and SOD2 genes. These findings indicate that nanoencapsulation with LNC increases the protective effects of melatonin against oxidative stress and cell apoptosis during in vitro embryo culture in bovine species.

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“…; Arias‐Alvarez et al. ) are improved. Recent study has shown that eCG did not to modify embryo gene expression patterns related to glucose intake, proliferation and differentiation, apoptotic‐induced pathways, capacity of implantation and foetal growth, but embryos from eCG‐treated mothers showed a lower antioxidant defence after the maternal eCG treatment (Arias‐Alvarez et al.…”

Section: Reproductive Strategiesmentioning

confidence: 99%

“…), oocyte cytoplasmic maturation (Arias‐Alvarez et al. ), ovulation rate and embryo development (Theau‐Clément et al. ; Arias‐Alvarez et al.…”

Section: Reproductive Strategiesmentioning

confidence: 99%

“…Recent study has shown that eCG did not to modify embryo gene expression patterns related to glucose intake, proliferation and differentiation, apoptotic‐induced pathways, capacity of implantation and foetal growth, but embryos from eCG‐treated mothers showed a lower antioxidant defence after the maternal eCG treatment (Arias‐Alvarez et al. ). Such oxidative events may be implicated in the reduced prolificacy and fertility rates observed in females when eCG administration is used repeated and consecutive times.…”

Section: Reproductive Strategiesmentioning

confidence: 99%

See 1 more Smart Citation

Reproductive and Nutritional Management on Ovarian Response and Embryo Quality on Rabbit Does

Lorenzo

García-García

Arias-Álvarez

et al. 2014

Reprod Domestic Animals

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Contents Rabbit does in modern rabbitries are under intensive reproductive rhythms. Females are high milk producers with high energetic expenses due to the extensive overlap between lactation and gestation. This situation leads to a negative energy balance with a mobilization of body fat especially in primiparous rabbit does. Poor body condition and poor health status severely affect the reproductive features (fertility rate and lifespan of the doe as well as ovarian physiology). This paper reviews some reproductive and nutritional approaches used in the last years to improve the reproductive performance of rabbit females, mainly focusing on the influence on ovarian response and embryo quality and with emphasis on epigenetic modifications in pre‐implantation embryos and offspring consequences.

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Ovarian response and embryo gene expression patterns after nonsuperovulatory gonadotropin stimulation in primiparous rabbits does

Cited by 15 publications

References 52 publications

Expression of placenta-specific 8 in human oocytes, embryos, and models of in vitro implantation

Expression of placenta-specific 8 in human oocytes, embryos, and models of in vitro implantation

Effects of Two Types of Melatonin-Loaded Nanocapsules with Distinct Supramolecular Structures: Polymeric (NC) and Lipid-Core Nanocapsules (LNC) on Bovine Embryo Culture Model

Reproductive and Nutritional Management on Ovarian Response and Embryo Quality on Rabbit Does

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