Ovariectomy-associated changes in interradicular septum and in tibia metaphysis in different observation periods in rats

Esteves, Carina M.; Moraes, Renata Mendonça; Gomes, Filipa; Marcondes, Meryenn Souza; Lima, Gabriela Moreira; Anbinder, Ana Lia

doi:10.1016/j.prp.2014.11.015

Cited by 15 publications

(14 citation statements)

References 37 publications

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“…The ovaries could not be located and the uteruses were very small, which indicated that OVX mice had their ovaries removed. As expected, OVX 8 , OVX 12 and OVX 16 were also heavier than mice in the SHAM group, although there was not a significant difference between OVX 16 and SHAM groups (P<0.01, P<0.05 and P=0.057, respectively; Table III). …”

Section: Agesupporting

confidence: 74%

Section: Agementioning

confidence: 90%

“…In general, microarchitectural deterioration was severe in OVX 8 , intermediate in OVX 12 and lowest in OVX 16 . This was highlighted by significant differences between OVX and SHAM across the parameters examined in OVX 8 , with the exception of Tb.Th and SMI. For trabecular vBMD, Tb.Sp and Conn.D in the distal femur, the response to OVX depended on the age, as determined by two-way analysis of variance analyses between treatment and age (P treatment*age =0.016, P treatment*age <0.001, P treatment*age =0.035, respectively).…”

Section: Agementioning

confidence: 90%

“…The ovariectomy (OVX) rat is the most commonly used animal model for evaluating the mechanisms underlying postmenopausal osteoporosis and therapeutic strategies for treating this disease (6)(7)(8)(9). However, compared with rats, mice may be a more effective model as they have a more easily manipulated genome and lower drug doses are required for treatment.…”

Section: Introductionmentioning

confidence: 99%

“…Mice were used in three different age groups (8,12 and 16 weeks old) to evaluate the extent of bone loss in response to OVX by micro-computed tomography (µCT). To further evaluate bone mass changes of C57BL/6J mice in response to OVX and estrogen replacement, the inbred strain (BALB/c) and outbred strains (ICR and Kunming) of mice were used in this study, and OVX was performed in those mice at 8 weeks of age.…”

Section: Introductionmentioning

confidence: 99%

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Age-dependent variations of cancellous bone in response to ovariectomy in C57BL/6J mice

et al. 2018

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Abstract. The ovariectomized (OVX) mouse model has been widely accepted to be suitable for the study of postmenopausal osteoporosis. However, whether C57BL/6J mice, a commonly used genetic background mouse strain, is an appropriate model for postmenopausal osteoporosis remains controversial. The present study investigated the effect of the OVX model on alterations in bone density and microarchitecture in C57BL/6J female mice of different ages. C57BL/6J mice were divided into 8-, 12-and 16-week-old groups (OVX 8 , OVX 12 and OVX 16 ) from the beginning of OVX. At 8 weeks post-surgery, the mice were anesthetized and micro-computed tomography was used to analyze the bone density and microarchitecture. The results revealed that OVX-induced loss of cancellous bone was greatest in OVX 8 , moderate in OVX 12 , and only a weak bone loss was observed in the OVX 16 group when compared with the SHAM 16 control group. In addition, the effect of genetic backgrounds in response to the OVX model were examined. Several other strains of mice, including inbred (BALB/c) and outbred (ICR and Kunming), were used in the present study, all of which were subjected to OVX at 8 weeks of age. The present findings revealed that the highest rate of bone loss was detected in C57BL/6J female mice. In addition, treatment with estrogen (17β-estradiol, 30 µg/kg five times per week) led to a significant increase in bone density in C57BL/6J mice compared with the other strains of mice. Therefore, these results may provide novel insights into the age-and strain-associated effect of OVX on regulating turnover of bone in female mice. The present findings also suggest 8-week-old C57BL/6J mice as an animal model for postmenopausal osteoporosis and preclinical testing of potential therapies for this disease.

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