Ovariectomy fails to augment bone resorption and marrow B lymphopoiesis in granulocyte colony-stimulating factor transgenic mice

Oda, Takashi; Wada, Takuro; Kuwabara, Hiromichi; Sawada, Norimasa; Yamashita, Toshihiko; Kokai, Yasuo

doi:10.1007/s00776-004-0851-y

Cited by 15 publications

(4 citation statements)

References 35 publications

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“…G-CSF also mobilizes hematopoietic precursor cells from bone marrow (62) and increases the number of circulating osteoclast precursors (63), which are likely related to increased osteoclast activity. Accordingly, G-CSF overexpression in mice inhibited the ability of osteoblasts to respond to BMP and increased bone resorption (64, 65). …”

Section: Introductionmentioning

confidence: 99%

Osteoimmunology: cytokines and the skeletal system

Lee

Kim²,

Choi

et al. 2008

BMB Reports

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It has become clear that complex interactions underlie the relationship between the skeletal and immune systems. This is particularly true for the development of immune cells in the bone marrow as well as the functions of bone cells in skeletal homeostasis and pathologies. Because these two disciplines developed independently, investigators with an interest in either often do not fully appreciate the influence of the other system on the functions of the tissue that they are studying. With these issues in mind, this review will focus on several key areas that are mediated by crosstalk between the bone and immune systems. A more complete appreciation of the interactions between immune and bone cells should lead to better therapeutic strategies for diseases that affect either or both systems. [BMB reports 2008; 41(7): 495-510]

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Section: Introductionmentioning

confidence: 99%

Osteoimmunology: cytokines and the skeletal system

Lee

Kim²,

Choi

et al. 2008

BMB Reports

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“…This increase in B lymphopoiesis ultimately translates into an elevation in mature bone marrow B cells [Garcia-Perez et al, 2006]. Furthermore, ovariectomy fails to augment bone marrow B lymphopoiesis concurrent with a failure to stimulate bone resorption in granulocyte colony-stimulating factor (G-CSF) transgenic mice [Oda et al, 2005], while the lymphopoietic cytokine IL-7 when administered to mice in vivo, induces B lymphopoiesis and osteoclastic bone destruction mimicking the osteoporosis associated with estrogen deficiency [Miyaura et al, 1997]. Such studies have led to the suggestion that stimulated B lymphopoiesis is a key player in ovariectomy-induced bone resorption [Onoe et al, 2000].…”

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confidence: 99%

Ovariectomy‐induced bone loss occurs independently of B cells

Yang

et al. 2006

J of Cellular Biochemistry

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Estrogen withdrawal is associated with a significant expansion in B cell precursor and mature B cell populations. However, despite significant circumstantial evidence the role of B lineage cells in ovariectomy-induced bone loss in vivo is unclear. In vitro studies have demonstrated that mature B cells have the potential to both positively and negatively impact osteoclastogenesis by virtue of their capacity to secrete pro-osteoclastogenic cytokines including receptor activator of NFkappaB ligand (RANKL), as well as anti-osteoclastogenic cytokines such as osteoprotegerin (OPG) and transforming growth factor beta (TGFbeta). Although several studies have suggested that expansion of the B lineage following ovariectomy may play a key role in the etiology of ovariectomy-induced bone loss, in vivo studies to directly test this notion have yet to be conducted. In this study, we performed ovariectomy on microMT(-/-) mice which are specifically deficient in mature B cells. Analysis of bone mineral density (BMD) by dual-energy X-ray absorptiometry (DXA) and micro-computed tomography (CT) demonstrate that mature B cell-deficient mice undergo an identical loss of bone mass relative to wild-type (WT) control mice. Our data demonstrate that mature B cells are not central mediators of ovariectomy-induced bone loss in vivo.

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“…Eight-week-old female C57BL/6 mice were ovariectomized (OVX) or sham-operated under general anesthesia as previously described [13]. Mice were sacrificed 4 weeks after surgery.…”

Section: Ovariectomymentioning

confidence: 99%

Reveromycin A Inhibits Bone Loss in Ovariectomized Mice

Ozasa¹,

Wada²,

Yamashita³

et al. 2019

J Surg Res

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Background and objectives: Reveromycin A inhibits bone metastasis of lung cancer cells in natural killer celldepleted immunodeficient mice with increasing apoptosis of osteoclasts. This knowledge suggests that reveromycin A is a potent inhibitor for osteoclasts in vivo. To determine the activity of bone loss in acute estrogen deficiency, we studied ovariectomized mice treated with reveromycin A.Materials and Methods: Eight-week-old female C57BL/6 mice were either ovariectomized or sham-operated.Reveromycin A was continuously administered for the first 2 weeks (Group RA) or the last 2 weeks (Group RP) using a subcutaneous mini osmotic pump. Mice were sacrificed 4 weeks after surgery. Inhibition was detected by images as well as serum carboxyterminal telopeptide of type I collagen (CTX).Results: BMD reduction in the RA group was significantly inhibited and was comparable with that in the sham group. In contrast, the RP group failed to inhibit BMD reduction. The plasma CTX level at the 28 th day of the experiment was significantly increased in the RP and OVX groups compared with that in the sham group, while the increase was inhibited in the RA group. Morphometric analysis revealed that absorption of trabecular bone was strongly inhibited in the RA group. Conclusion:The inhibitory effects of reveromycin A administered immediately after ovariectomy was the most effective at the early phase of bone loss than at the later stage. A new reagent that inhibits bone loss in acute estrogen deficiency might be a new tool to experimentally study osteoporosis and may have clinical implications.

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Ovariectomy fails to augment bone resorption and marrow B lymphopoiesis in granulocyte colony-stimulating factor transgenic mice

Cited by 15 publications

References 35 publications

Osteoimmunology: cytokines and the skeletal system

Osteoimmunology: cytokines and the skeletal system

Ovariectomy‐induced bone loss occurs independently of B cells

Reveromycin A Inhibits Bone Loss in Ovariectomized Mice

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