Over-expression of Osmotin Induces Proline Accumulation and Confers Tolerance to Osmotic Stress in Transgenic Tobacco

Barthakur, Sharmistha; Babu, Vidhu Sankar; Bansa, Kailash C.

doi:10.1007/bf03263103

Cited by 86 publications

(64 citation statements)

References 19 publications

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“…Osmotin and osmotin-like proteins can also be activated by microbial infections and by various abiotic stresses (Velazhahan et al 1999). This has led to studies on the transformation and overexpression of osmotin and osmotin-like genes in a number of plants, and drought-, salt-, cold-and disease-tolerant crops, such as tobacco (Barthakur et al 2001), tomato (Sarad et al 2004, strawberry (Zhang and Shih 2007;Husaini and Abdin 2008), wheat (Noori and Sokhansanj 2008) and cotton (Parkhi et al 2009), have been obtained. As osmotin is a PR-5 protein, its overexpression has been found to delay the development of disease symptoms with Phytophthora infestans causing late blight disease of potato (Liu et al 1994).…”

Section: Discussionmentioning

confidence: 98%

“…Osmotin and osmotin-like genes have a dual function in osmotic stress and plant defense (Konowicz et al 1992;La Rosa et al 1992;Nelson et al 1992;Zhu et al 1995), which has led to studies aimed at transforming and overexpressing osmotin in plants of agronomical importance. Tobacco osmotin has been shown to confer tolerance to NaCl and drought stress in transgenic tobacco (Barthakur et al 2001), strawberry (Husaini and Abdin 2008), wheat (Noori and Sokhansanj 2008) and cotton (Parkhi et al 2009). As a PR protein, tobacco osmotin is able to inhibit the growth of Candida albicans, Neurospora crassa and Trichoderma reesei (Vigers et al 1991).…”

Section: Introductionmentioning

confidence: 98%

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High-efficiency transformation and selective tolerance against biotic and abiotic stress in mulberry, Morus indica cv. K2, by constitutive and inducible expression of tobacco osmotin

et al. 2010

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Osmotin and osmotin-like proteins are stress proteins belonging to the plant PR-5 group of proteins induced in several plant species in response to various types of biotic and abiotic stresses. We report here the overexpression of tobacco osmotin in transgenic mulberry plants under the control of a constitutive promoter (CaMV 35S) as well as a stress-inducible rd29A promoter. Southern analysis of the transgenic plants revealed the stable integration of the introduced genes in the transformants. Real-time PCR analysis provided evidence for the expression of osmotin in the transgenic plants under both the constitutive and stress-inducible promoters. Transgenic plants with the stress-inducible promoter were observed to better tolerate salt and drought stress than those with the constitutive promoter. Transgenic plants when subjected to simulated salinity and drought stress conditions showed better cellular membrane stability (CMS) and photosynthetic yield than non-transgenic plants under conditions of both salinity and drought stress. Proline levels were very high in transgenic plants with the constitutive promoter relative to those with the stress-inducible promoter. Fungal challenge undertaken with three fungal species known to cause serious losses to mulberry cultivation, namely, Fusarium pallidoroseum, Colletotrichum gloeosporioides and Colletotrichum dematium, revealed that transgenic plants with osmotin under control of the constitutive promoter had a better resistance than those with osmotin under the control of the stress-inducible promoter. Evaluation in next generation was undertaken by studying bud break in transgenic and non-transgenic plants under simulated drought (2% polyethylene glycol) and salt stress (200 mM NaCl) conditions. The axillary buds of the selected transgenic lines had a better bud break percentage under stressed conditions than buds from non-transgenic mulberry lines. A biotic assay with Bombyx mori indicated that osmotin protein had no undesirable effect on silkworm rearing and feeding. We therefore conclude that 35S transgenic plants are better suited for both abiotic stress also biotic challenges (fungal), while the rd29A transgenic plants are more responsive to drought.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 98%

High-efficiency transformation and selective tolerance against biotic and abiotic stress in mulberry, Morus indica cv. K2, by constitutive and inducible expression of tobacco osmotin

et al. 2010

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“…The osmotin gene is induced by fungal and viral protein in addition to osmotic stress as a result of dehydration, high salt and cold stress (LaRosa et al 1992). Transgenic tobacco plants overexpressing osmotin gene showed retarded leaf senescence and improved seed germination under salt stress (Barthakur et al 2001(Barthakur et al , 2002. Transgenic rice containing the coding region of a thaumatin-like protein (TLP-D34), a PR-5 protein conferred enhanced fungal protection and osmotic adjustment in rice (Datta et al 2000).…”

Section: Protection Factors Of Macromoleculesmentioning

confidence: 97%

Transgenic plants with improved dehydration-stress tolerance: Progress and future prospects

Cherian¹,

Reddy²,

Ferreira³

2006

Biologia plant.

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This review summarizes the recent progress made towards the development of transgenic plants with improved tolerance to water stress and salinity. Of the various strategies employed, emphasis has been given to the genes engineered for the biosynthesis of osmoprotectants and osmolytes. This review also briefly discusses the importance of the use of specific stress inducible promoters and the future prospects of transgenic plants with improved agronomic traits.

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“…The overexpression of TBOSM has been shown to confer resistance to osmotic stress (Barthakur et al 2001), salt tolerance, drought stress (Goel et al 2010), and fungal development in plants (Li et al 1998). TBOSM has been successfully expressed in solanaceous plants (Barthakur et al 2001, Goel et al 2010, Li et al 1998, Sharma et al 2015; however, there are few if any reports of transgenic expression of osmotin in monocotyledonous plants. The expression of TBOSM or an osmotin-like PR protein in sugarcane may assist to improve the fungal tolerance of the crop.…”

Section: Disease Resistancementioning

confidence: 99%

Sugarcane biotechnology: tapping unlimited potential

Bhowmik

Brinin

Williams

2016

Sugarcane‐Based Biofuels and Bioproducts

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Over-expression of Osmotin Induces Proline Accumulation and Confers Tolerance to Osmotic Stress in Transgenic Tobacco

Cited by 86 publications

References 19 publications

High-efficiency transformation and selective tolerance against biotic and abiotic stress in mulberry, Morus indica cv. K2, by constitutive and inducible expression of tobacco osmotin

High-efficiency transformation and selective tolerance against biotic and abiotic stress in mulberry, Morus indica cv. K2, by constitutive and inducible expression of tobacco osmotin

Transgenic plants with improved dehydration-stress tolerance: Progress and future prospects

Sugarcane biotechnology: tapping unlimited potential

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