Overall condition improvement in a rat model of nephrotic syndrome treated with CellCept nanoliposomes

Abstract

Purpose To investigate the effect of CellCept nanoliposomes on Adriamycin-induced nephrotic syndrome in rats. Methods To model nephrotic syndrome, rats were injected with 6.5 mg/kg of Adriamycin in the tail vein. The rats were randomly divided into three groups, including a control group, a free mycophenolate mofetil (MMF)-treated group, and a liposome-encapsulated MMF-treated group. Five weeks after the Adriamycin treatment, the free MMF-treated group received CellCept while the liposome-encapsulated MMF-treated group received the CellCept nanoliposomes for 2 weeks. The general condition of the animals was observed, which included urine volume over 24 h, urine protein levels, and serum biochemical indexes. Renal morphology was also observed. Results The level of urine protein over 24 h was increased in the control group, while plasma albumin (ALB) was decreased. The total cholesterol (TC) and triacylglycerol (TG) levels increased significantly (P < 0.05, P < 0.01). The pathological examination of the kidneys showed some abnormalities. In contrast, these parameters were improved significantly in the free mycophenolate mofetil (MMF)-treated and liposome-contained mycophenolate mofetil (MMF)-treated groups. Conclusion The CellCept nanoliposomes have a good therapeutic effect on Adriamycin-induced nephrotic syndrome in rats.

Yue-bi-tang attenuates adriamycin-induced nephropathy edema through decreasing renal microvascular permeability via inhibition of the Cav-1/ eNOS pathway

“…After 1 week of acclimatization, apart from the 10 rats randomized into the control group with saline (1.0 mL/100 g), we intravenously administered the rest with Adriamycin (ADR, 6.5 mg/kg dissolved in saline, Shenzhen Main Luck Pharmaceuticals Inc.) to establish a NS rat model ( Teng et al, 2017 ). In other words, a nephritis model was established by injecting Adriamycin in the caudal vein only once.…”

“…Full scan mode was adopted: scan range 80-1200 m/z. saline, Shenzhen Main Luck Pharmaceuticals Inc.) to establish a NS rat model (Teng et al, 2017). In other words, a nephritis model was established by injecting Adriamycin in the caudal vein only once.…”

A Tissue-Tended Mycophenolate-Modified Nanoparticle Alleviates Systemic Lupus Erythematosus in MRL/Lpr Mouse Model Mainly by Promoting Local M2-Like Macrophagocytes Polarization

“…Nanotechnology has been applied to protect and precisely deliver MMF/MPA to target tissues and cells to treat inflammatory diseases and has shown promise. [14][15][16][17][18][19][20] For example, a triglyceride mimetic prodrug of MPA has been developed for targeted delivery to mesenteric lymph nodes and successfully suppressed the immune reaction to an oral ovalbumin antigen challenge in mice. 17 In addition, MMF-loaded PEG-PLGA nanoparticles (NPs) have been perfused into a donor mouse heart prior to transplantation and successfully inhibited post-transplantation inflammation.…”