Overexpressed CISD2 has prognostic value in human gastric cancer and promotes gastric cancer cell proliferation and tumorigenesis <i>via</i> AKT signaling pathway

Wang, Lan; Ouyang, Fei; Liu, Xiaobo; Wu, Shu; Wang, Hongmei; Xu, Yuandong; Wang, Bin; Zhu, Jinrong; Xu, Xuehu; Zhang, Liang

doi:10.18632/oncotarget.6302

Cited by 47 publications

(41 citation statements)

References 32 publications

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“…Enhanced expression of the 2Fe-2S protein NAF-1 is associated with the onset and progression of multiple cancer types (10,11,(14)(15)(16)(17)(18)(19). To determine whether elevated NAF-1 levels play a direct role in tumor development, we stably overexpressed NAF-1 in MDA-MB-231 cells [NAF-1(+)] and generated xenograft tumors in nude mice.…”

Section: Naf-1 Overexpression Results In a Dramatic Augmentation In Xmentioning

confidence: 99%

“…Taken together, our findings suggest that any drastic change in the lability of the NAF-1 cluster would impair its function in maintaining the levels of iron and ROS in mitochondria under control. Altering NAF-1 cluster lability via different drugs could therefore be used to treat different cancers that depend on NAF-1 overexpression for their proliferation (10,11,(14)(15)(16)(17)(18)(19).…”

Section: Discussionmentioning

confidence: 99%

“…NAF-1 in gastric (16) cancer cells significantly enhanced cellular proliferation. Moreover, patients with gastric and liver cancer with high NAF-1 expression displayed a shorter survival and higher recurrence rate compared with patients with low NAF-1 expression (16,18). Taken together, these studies point to a potential key role for the NEET proteins NAF-1 and mNT in promoting the proliferation of different epithelial cancers.…”

mentioning

confidence: 99%

“…Enhanced expression of NAF-1 or mNT is associated with different cancers, including breast (10,11,14), prostate (15), gastric (16), cervical (17), liver (18), and laryngeal cancer (19). Silencing of mNT or NAF-1 expression in breast (10,11) or gastric (16) cancer cells significantly inhibited cellular proliferation and tumorigenicity, whereas overexpression of mNT in breast (14) or Significance Elevated expression of the iron-sulfur (Fe-S) protein nutrientdeprivation autophagy factor-1 (NAF-1) is associated with the progression of multiple cancer types.…”

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confidence: 99%

“…Silencing of mNT or NAF-1 expression in breast (10,11) or gastric (16) cancer cells significantly inhibited cellular proliferation and tumorigenicity, whereas overexpression of mNT in breast (14) or Significance Elevated expression of the iron-sulfur (Fe-S) protein nutrientdeprivation autophagy factor-1 (NAF-1) is associated with the progression of multiple cancer types. Here we demonstrate that the lability of the Fe-S cluster of NAF-1 plays a key role in promoting breast cancer cell proliferation, tumor growth, and resistance of cancer cells to oxidative stress.…”

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confidence: 99%

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Breast cancer tumorigenicity is dependent on high expression levels of NAF-1 and the lability of its Fe-S clusters

Darash-Yahana

Pozniak

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

127

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Iron-sulfur (Fe-S) proteins are thought to play an important role in cancer cells mediating redox reactions, DNA replication, and telomere maintenance. Nutrient-deprivation autophagy factor-1 (NAF-1) is a 2Fe-2S protein associated with the progression of multiple cancer types. It is unique among Fe-S proteins because of its 3Cys-1His cluster coordination structure that allows it to be relatively stable, as well as to transfer its clusters to apo-acceptor proteins. Here, we report that overexpression of NAF-1 in xenograft breast cancer tumors results in a dramatic augmentation in tumor size and aggressiveness and that NAF-1 overexpression enhances the tolerance of cancer cells to oxidative stress. Remarkably, overexpression of a NAF-1 mutant with a single point mutation that stabilizes the NAF-1 cluster, NAF-1(H114C), in xenograft breast cancer tumors results in a dramatic decrease in tumor size that is accompanied by enhanced mitochondrial iron and reactive oxygen accumulation and reduced cellular tolerance to oxidative stress. Furthermore, treating breast cancer cells with pioglitazone that stabilizes the 3Cys-1His cluster of NAF-1 results in a similar effect on mitochondrial iron and reactive oxygen species accumulation. Taken together, our findings point to a key role for the unique 3Cys-1His cluster of NAF-1 in promoting rapid tumor growth through cellular resistance to oxidative stress. Cluster transfer reactions mediated by the overexpressed NAF-1 protein are therefore critical for inducing oxidative stress tolerance in cancer cells, leading to rapid tumor growth, and drugs that stabilize the NAF-1 cluster could be used as part of a treatment strategy for cancers that display high NAF-1 expression.Fe-S | ROS | NEET | cancer | NAF-1

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Section: Naf-1 Overexpression Results In a Dramatic Augmentation In Xmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Breast cancer tumorigenicity is dependent on high expression levels of NAF-1 and the lability of its Fe-S clusters

Darash-Yahana

Pozniak

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

127

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Circular RNA circ‐PRKCI functions as a competitive endogenous RNA to regulate AKT3 expression by sponging miR‐3680‐3p in esophageal squamous cell carcinoma

Shi¹,

Shan²,

et al. 2019

J of Cellular Biochemistry

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Circular RNA (circRNA) is a new noncoding RNAs and plays an important role in many pathological processes. Recently, studies have shown that circular RNA_PRKCI (circ-PRKCI) regulates cell proliferation and cell migration of tumor cells. Esophageal carcinoma is a highly malignant digestive tract tumor, which is divided into esophageal adenocarcinoma and esophageal squamous cell carcinoma. In this study, we studied whether circ-PRKCI might influence cell proliferation and cell migration in esophageal squamous cell carcinoma.Quantitative reverse transcription PCR was performed to detect the relative expression of circ-PRKCI in five cases of esophageal squamous cell carcinoma and five cases of paired adjacent normal tissues. RNA immunoprecipitation assay and Luciferase assay confirm the direct interaction between miR-3680-3p and AKT3 or circ-PRKCI. Ethynyldeoxyuridine assays and cell counting Kit-8 were performed to evaluate the effect of miR-3680-3p or circ-PRKCI on cell proliferation, transwell assays were also performed to detect migration in vitro.We found circ-PRKCI is obviously upregulated in esophageal squamous cell carcinoma and upregulation of circ-PRKCI stimulated cell migration and proliferation of ESCC cells. In the mechanism, we confirm that circ-PRKCI, as a molecular sponge of miR-3680-3p, upregulates the expression of AKT. In conclusion, our current studies have been revealing that circ-PRKCI/miR-3680-3p/AKT3 regulatory network plays an important role in esophageal squamous cell carcinoma and that provide new insights into the pathogenesis of esophageal squamous cell carcinoma. K E Y W O R D S cell migration, cell proliferation, circular RNA_PRKCI (circ-PRKCI), esophageal squamous cell carcinoma, miR-3680-3p

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Multi‐omics analysis reveals that ferroptosis‐related gene CISD2 is a prognostic biomarker of head and neck squamous cell carcinoma

Wang

Huang

et al. 2023

The Journal of Gene Medicine

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BackgroundHead and neck squamous cell carcinoma (HNSCC) is a prevalent malignancy, with high mortality rate and unavailability of accurate therapies. However, its early prevention remains a challenge. In the purview of predictive, preventive, and personalized medicine (PPPM), it is paramount to identify novel and powerful biomarkers. CISD2 is a crucial regulator of iron homeostasis and reactive oxygen species (ROS). Recent studies showed that the NEET protein (NAF‐1) encoded by CISD2 is involved in regulating the proliferation and metastasis of tumor cells. Nevertheless, the prognostic value and immunological correlations of CISD2 remain unclear.MethodsBioinformatics analyses conducted utilizing data from comprehensive databases The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO). All statistical evaluations were executed employing R software.ResultsOur investigation of biological function, enrichment pathway, and immune correlation revealed a discernable linkage between CISD2 and the immune response. Moreover, we found that the suppression of CISD2 is associated with immune cell infiltration and various immune signatures.ConclusionsThe present study successfully revealed the potential prognostic and biological function of CISD2 in HNSCC. High expression of CISD2 are linked to gender, race, grade, etc., can notably enhance the early detection, prognosis, and prediction for individuals afflicted with HNSCC.

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Overexpressed CISD2 has prognostic value in human gastric cancer and promotes gastric cancer cell proliferation and tumorigenesis via AKT signaling pathway

Cited by 47 publications

References 32 publications

Breast cancer tumorigenicity is dependent on high expression levels of NAF-1 and the lability of its Fe-S clusters

Breast cancer tumorigenicity is dependent on high expression levels of NAF-1 and the lability of its Fe-S clusters

Circular RNA circ‐PRKCI functions as a competitive endogenous RNA to regulate AKT3 expression by sponging miR‐3680‐3p in esophageal squamous cell carcinoma

Multi‐omics analysis reveals that ferroptosis‐related gene CISD2 is a prognostic biomarker of head and neck squamous cell carcinoma

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