Overexpression of a Malus baccata MYB Transcription Factor Gene MbMYB4 Increases Cold and Drought Tolerance in Arabidopsis thaliana

Yao, Chunya; Li, Xingguo; Li, Yingmei; Gao, Yang; Liu, Wanda; Shao, Bangtao; Zhong, Jiliang; Huang, Pengfei; Han, Deguo

doi:10.3390/ijms23031794

Cited by 43 publications

(19 citation statements)

References 55 publications

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“…In the whole life cycle of plants, they are often affected by various environmental factors that are not conducive to growth, such as saline alkali conditions, drought, high temperature, iron deficiency, cold and lack of nutrition [ 1 , 2 , 3 , 4 , 5 , 6 , 7 ]. In order to survive under various adverse conditions and carry out normal life activities, plants will produce different adaptation mechanisms [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Overexpression of MxbHLH18 Increased Iron and High Salinity Stress Tolerance in Arabidopsis thaliana

Liang

Yao

et al. 2022

IJMS

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In the life cycle of apple, it will suffer a variety of abiotic stresses, such as iron stress and salt stress. bHLH transcription factors (TFs) play an indispensable role in the response of plants to stress. In this study, a new bHLH gene named MxbHLH18 was separated from Malus xiaojinensis. According to the results of subcellular localization, MxbHLH18 was localized in the nucleus. Salt stress and iron stress affected the expression of MxbHLH18 in Malus xiaojinensis seedlings to a large extent. Due to the introduction of MxbHLH18, the resistance of Arabidopsis thaliana to salt, high iron and low iron was significantly enhanced. Under the environmental conditions of high iron and low iron, the overexpression of MxbHLH18 increased many physiological indexes of transgenic Arabidopsis compared to wild type (WT), such as root length, fresh weight and iron content. The high level expression of MxbHLH18 in transformed Arabidopsis thaliana can not only increased the content of chlorophyll and proline, as well as increasing the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT); it also reduced the content of malondialdehyde (MDA), which was more obvious under high salt conditions. In addition, the relative conductivity, H2O2 content and O2− content in transgenic Arabidopsis decreased under salt stress. Meanwhile, MxbHLH18 can also regulate the expression of downstream genes associated with salt stress (AtCBF1/2/3, AtKIN1 and AtCOR15a/b) and iron stress (AtIRT1, AtFRO2, AtNAS2, ATACT2, AtZIF1 and AtOPT3). Therefore, MxbHLH18 can actively promote the adaptability of plants to the growth environment of salt and low and/or iron.

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Section: Introductionmentioning

confidence: 99%

Overexpression of MxbHLH18 Increased Iron and High Salinity Stress Tolerance in Arabidopsis thaliana

Liang

Yao

et al. 2022

IJMS

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“…The transcription factor is mainly responsible for sensing and transducing early responses to salt stress in plants, such as DREB (dehydration responsive element binding factors), bZIP (basic leucine zipper), MYB (v-myb avian myeloblastosis viral oncogene homolog), and so forth. As one of the most important transcription factor families in plants, MYB transcription factors are widely involved in abiotic stress resistance, such as salt stress, drought stress and so on ( Yu et al, 2017 ; Yao et al, 2022 ). Some studies have confirmed that overexpression of MYB gene can improve the salt tolerance of transgenic plants ( Shen et al, 2018 ; Liu et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of Long Non-coding RNA in Tomato Roots Under Salt Stress

Wang

et al. 2022

Front. Plant Sci.

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As one of the most important vegetable crops in the world, the production of tomatoes was restricted by salt stress. Therefore, it is of great interest to analyze the salt stress tolerance genes. As the non-coding RNAs (ncRNAs) with a length of more than 200 nucleotides, long non-coding RNAs (lncRNAs) lack the ability of protein-coding, but they can play crucial roles in plant development and response to abiotic stresses by regulating gene expression. Nevertheless, there are few studies on the roles of salt-induced lncRNAs in tomatoes. Therefore, we selected wild tomato Solanum pennellii (S. pennellii) and cultivated tomato M82 to be materials. By high-throughput sequencing, 1,044 putative lncRNAs were identified here. Among them, 154 and 137 lncRNAs were differentially expressed in M82 and S. pennellii, respectively. Through functional analysis of target genes of differentially expressed lncRNAs (DE-lncRNAs), some genes were found to respond positively to salt stress by participating in abscisic acid (ABA) signaling pathway, brassinosteroid (BR) signaling pathway, ethylene (ETH) signaling pathway, and anti-oxidation process. We also construct a salt-induced lncRNA-mRNA co-expression network to dissect the putative mechanisms of high salt tolerance in S. pennellii. We analyze the function of salt-induced lncRNAs in tomato roots at the genome-wide levels for the first time. These results will contribute to understanding the molecular mechanisms of salt tolerance in tomatoes from the perspective of lncRNAs.

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“…Among the other species, GaMYB85 of Gossypium aridum , homolog with AtMYB85 from S8, reportedly play an important role in the drought tolerance of plants [ 34 ]. R2R3-MYB gene MdoMYB121 from Malus domestica , MbMYB4 from M. baccata, and PlMYB108 (S20) from Paeonia lactiflflora were involved in increasing drought tolerance [ 35 , 36 , 37 ]. Notably, these functional genes were detected in S8 and S20.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification Analysis of the R2R3-MYB Transcription Factor Family in Cymbidium sinense for Insights into Drought Stress Responses

Zhu

Wang

et al. 2023

IJMS

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Cymbidium sinense represents a distinctive Orchidaceae plant that is more tolerant than other terrestrial orchids. Studies have shown that many members of the MYB transcription factor (TF) family, especially the R2R3-MYB subfamily, are responsive to drought stress. This study identified 103 CsMYBs; phylogenetic analysis classified these genes into 22 subgroups with Arabidopsis thaliana. Structural analysis showed that most CsMYB genes contained the same motifs, three exons and two introns, and showed a helix-turn-helix 3D structure in each R repeat. However, the members of subgroup 22 contained only one exon and no intron. Collinear analysis revealed that C. sinense had more orthologous R2R3-MYB genes with wheat than A. thaliana and rice. Ka/Ks ratios indicated that most CsMYB genes were under purifying negative selection pressure. Cis-acting elements analysis revealed that drought-related elements were mainly focused on subgroups 4, 8, 18, 20, 21, and 22, and Mol015419 (S20) contained the most. The transcriptome analysis results showed that expression patterns of most CsMYB genes were upregulated in leaves in response to slight drought stress and downregulated in roots. Among them, members in S8 and S20 significantly responded to drought stress in C. sinense. In addition, S14 and S17 also participated in these responses, and nine genes were selected for the real-time reverse transcription quantitative PCR (RT-qPCR) experiment. The results were roughly consistent with the transcriptome. Our results, thus, provide an important contribution to understanding the role of CsMYBs in stress-related metabolic processes.

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Overexpression of a Malus baccata MYB Transcription Factor Gene MbMYB4 Increases Cold and Drought Tolerance in Arabidopsis thaliana

Cited by 43 publications

References 55 publications

Overexpression of MxbHLH18 Increased Iron and High Salinity Stress Tolerance in Arabidopsis thaliana

Overexpression of MxbHLH18 Increased Iron and High Salinity Stress Tolerance in Arabidopsis thaliana

Identification and Characterization of Long Non-coding RNA in Tomato Roots Under Salt Stress

Genome-Wide Identification Analysis of the R2R3-MYB Transcription Factor Family in Cymbidium sinense for Insights into Drought Stress Responses

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