Overexpression of AtOxR gene improves abiotic stresses tolerance and vitamin C content in Arabidopsis thaliana

Abstract

BackgroundAbiotic stresses are serious threats to plant growth, productivity and result in crop loss worldwide, reducing average yields of most major crops. Although abiotic stresses might elicit different plant responses, most induce the accumulation of reactive oxygen species (ROS) in plant cells leads to oxidative damage. L-ascorbic acid (AsA, vitamin C) is known as an antioxidant and H2O2-scavenger that defends plants against abiotic stresses. In addition, vitamin C is also an important component of human nutrition that has to be obtained from different foods. Therefore, increasing the vitamin C content is important for improving abiotic stresses tolerance and nutrition quality in crops production.ResultsHere, we show that the expression of AtOxR gene is response to multiple abiotic stresses (salt, osmotic, metal ion, and H2O2 treatment) in both the leaves and roots of Arabidopsis. AtOxR protein was localized to the Endoplasmic Reticulum (ER) in yeast and Arabidopsis cells by co-localization analysis with ER specific dye. AtOxR-overexpressing transgenic Arabidopsis plants enhance the tolerance to abiotic stresses. Overexpression of AtOxR gene resulted in AsA accumulation and decreased H2O2 content in transgenic plants.ConclusionsIn this study, our results show that AtOxR responds to multiple abiotic stresses. Overexpressing AtOxR improves tolerance to abiotic stresses and increase vitamin C content in Arabidopsis thaliana. AtOxR will be useful for the improvement of important crop plants through moleculer breeding.Electronic supplementary materialThe online version of this article (doi:10.1186/s12896-016-0299-0) contains supplementary material, which is available to authorized users.

Kiwifruit MYBS1‐like and GBF3 transcription factors influence l‐ascorbic acid biosynthesis by activating transcription of GDP‐L‐galactose phosphorylase 3

“…In plants, l ‐ascorbic acid (AsA; vitamin C) is a major antioxidant with roles in photosynthesis, respiration, cell division, growth regulation, integrating phytohormone signalling and ageing (Sanmartin et al ., 2003; Barth et al ., 2006; Kotchoni et al ., 2009; Smirnoff, 2011; Hanxia et al ., 2015; Bu et al ., 2016; Y. Yu et al ., 2019; Zhang et al ., 2020; Bulley et al ., 2021). As an essential micronutrient that humans have lost the ability to produce, AsA is required for key metabolic functions in humans and must be obtained from the diet, mainly from fruits and vegetables.…”

A Biostimulant Obtained from the Seaweed Ascophyllum nodosum Protects Arabidopsis thaliana from Severe Oxidative Stress

“…In addition, higher levels of AtOXR, which encodes transmembrane OXR protein, was observed in SF-primed plants ( Table 1). Overexpression of AtOXR in Arabidopsis results in ascorbate accumulation, concomitant with an increased tolerance to oxidative stress [63]. Notably, expression of 1-myo-inositol-1-phosphate synthase encoding MIPS1 was elevated in SF-primed plants.…”

Mining Beneficial Genes for Salt Tolerance From a Core Collection of Rice Landraces at the Seedling Stage Through Genome-Wide Association Mapping

“…In addition, we also found that the homologous gene AT5G57345 in Arabidopsis , a single copy gene, is localized to ER and expressed in the whole plant and induced expression in response to abiotic stress. Although the function of AT5G57345 is unclear, overexpression can lead to increased tolerance to abiotic stress and increased ascorbic acid content ( Bu et al, 2016 ). In addition, we also found that the expression level of LOC_Os06g47970 in S125 was much higher than that in S87.…”