Overexpression of GhDof1 improved salt and cold tolerance and seed oil content in Gossypium hirsutum

The role of gibberellin synthase geneGhGA2ox1in upland cotton (Gossypium hirsutumL.) responses to drought and salt stress

“…In this research, a higher level of chlorophyll, relative water, and free proline content was detected in transgenic plant under the drought and salt stress. A number of studies have shown that increase in free proline content of plant can enhance the tolerance to abiotic stress , and chlorophyll content and RWC of leaves in plants are closely related to the biosynthesis of GA . In this study, after drought and salt stress treatment, the loss of chlorophyll and RWC in transgenic plants was less than that in non‐transgenic plant, and the free proline accumulation in transgenic plants was higher than that in non‐transgenic plant.…”

Identification and analysis of oil candidate genes revealed the molecular basis of oil accumulation in Gossypium hirsutum L. for cottonseed biofuels development

“…Integration of KEGG, BLAST and RNA-seq analysis results, 19 credible candidate genes related to cottonseed oil accumulation were ultimately obtained (Additional le 5: Table S5). Of these, transcription factor HSL1 were found to regulate a series of reactions in oil synthesis in previous researches for oilseed crops [29,48]. This indicated that GhHSL1 may play a signi cant role for oil synthesis in developing cotton ovules.…”

“…In the past several decades, some genes and TFs in oil crops also have been veri ed to be associated with lipid synthesis based on the Arabidopsis thaliana oil-related genes through multi-omics analyses. For instance, phosphoenolpyruvate carboxylase (PEPC) in upland cotton for the formation of pyruvate [17]; acetyl-CoA carboxylase (ACCase) in rapeseed [18], cotton [19], and potato [20] participate in de novo FA synthesis; fatty acylthioesterase B (FATB) in soybean [21] and cotton [22], and β-ketoacyl-ACP synthase I (KASI) and long chain acyl-CoA synthetase 9 (LACS9) in soybean [23], and KASII [24], Stearyl-ACPdesaturase (SAD) [25], and GhWRI1 [26] in cotton are involved in FA synthesis; glycerol-3-phosphate dehydrogenase (GPDH) in rapeseed [27], glycerol-3-phosphate acyltransferase (GPAT) [28] and GhDof1 [29] in cotton, 2-lysophosphatidic acid acyltransferase (LPAAT) in cotton [30], and OLEs in soybean [31] are reported to be related to TAG synthesis. Recently, Liu et al [32] identi ed GmPDAT, GmAGT, GmACP4, GmZF351 and GmPgs1 ve soybean seed oil related genes using an innovative three-dimension network construction approach, as well as genome-wide association studies and multi-omics analyses.…”

GhCIPK6 Increases Salt Tolerance in Transgenic Upland Cotton by Involving in ROS Scavenging and MAPK Signaling Pathways

“…Plants initiate a series of adaptive mechanism and survival responses to abiotic stresses, and result in serial changes in gene expression [3][4][5][6]. These stress-response genes involve Ca 2+ -sensors [7,8], transcription factors [9,10], protein kinases [11][12][13], osmotic proteins [14][15][16], and hydroperoxidases [17,18], in which protein kinases play a particularly important role in plant response. A group of serine-threonine kinases, designated as CBLinteracting protein kinases (CIPKs), which interact speci cally with calcineurin B-like proteins (CBLs), has been characterized in plant genomes [19,20].…”