Overexpression of long non‑coding RNA colon cancer‑associated transcript 2 is associated with advanced tumor progression and poor prognosis in patients with colorectal cancer

Zhang, Junling; Jiang, Yong; Zhu, Jing; Wu, Tao; Ma, Jiachi; Du, Chuang; Chen, Shanwen; Li, Tengyu; Han, Jinsheng; Wang, Xin

doi:10.3892/ol.2017.7049

Cited by 20 publications

(22 citation statements)

References 27 publications

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“…In addition, colon cancer patients with high miR-937 expression showed shorter overall survival than those patients with low miR-937 expression. Multivariate Cox regression analysis results also showed lymph node metastasis, vascular invasion, and TNM stage were independent prognostic factors in colon cancer, which is consistent with previous studies [28, 29]. Importantly, multivariate Cox regression analysis results showed miR-937 was an independent prognostic factor in colon cancer.…”

Section: Discussionsupporting

confidence: 90%

MicroRNA-937 is overexpressed and predicts poor prognosis in patients with colon cancer

Liu

Wang

et al. 2019

Diagn Pathol

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Background: Colon cancer is a heterogeneous tumor and a leading cause of cancer-related mortality. MicroRNA (miRNA) has been proposed as the biomarker in cancers. The aim of this study was to investigate the clinical significance and potential functional role of miR-937 in colon cancer. Methods: In the present study, reverse transcription-quantitative polymerase chain reaction (qRT-PCR) was conducted to examine the expression levels of miR-937 in colon cancer tissues and cell lines. Kaplan-Meier curve and Cox regression analyses were used to determine the prognostic impact of miR-937 on survival. Cell Counting Kit-8 and Transwell assays were performed to examine cell proliferation, migration, and invasion, respectively. Results: miR-937 was significantly upregulated in colon cancer tissues and cell lines. Clinical analysis results showed that miR-937 expression was associated with lymph node metastasis and TNM stage. Patients with high miR-937 expression predicted a shorter overall survival rate. Functionally, overexpression of miR-937 promoted cell proliferation, migration, and invasion, while inhibition of miR-937 inhibited these cellular behaviors in vitro. Conclusions: These results suggested that miR-937 may act as a prognostic biomarker and a potential target for therapeutic strategy, as well as promote proliferation, migration, and invasion of colon cancer.

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Section: Discussionsupporting

confidence: 90%

MicroRNA-937 is overexpressed and predicts poor prognosis in patients with colon cancer

Liu

Wang

et al. 2019

Diagn Pathol

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“…cells, and ZNFX1-AS1 knockdown inhibited cell proliferation. In addition, previous studies have indicated that increased lncRNA-CCAT2 expression may serve as a potential biomarker for CRC diagnosis, and may also serve as an independent predictor of prognosis (36,37). In the present study, CCAT1 expression was significantly upregulated in CRC tissue.…”

Section: Discussionsupporting

confidence: 62%

Long non‑coding RNA CCAT1 promotes colorectal cancer cell migration, invasiveness and viability by upregulating VEGF via negative modulation of microRNA‑218

Zou

et al. 2020

Exp Ther Med

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Increasing evidence has demonstrated that long non-coding (lnc) RNA is aberrantly expressed in numerous types of cancer. Colorectal cancer is a common malignancy; however, the role and mechanism underlying the influence of lncRNA-colon cancer associated transcript 1 (CCAT1) in colorectal cancer is yet to be elucidated. The present study revealed that CCAT1 is highly expressed in colorectal cancer tissues. Bioinformatics analysis and a dual-luciferase reporter gene assay indicated that CCAT1 and microRNA (miR)-218 had complementary binding sites. Furthermore, reverse transcription-quantitative PCR revealed that miR-218 was downregulated in colorectal cancer tissues compared with paired adjacent healthy tissues. To investigate the biological effects of CCAT1 on colorectal cancer cells, MTT and Transwell assays were performed. The results revealed that when compared with the control group, CCAT1-short hairpin (sh)RNA significantly inhibited colorectal cancer cell (SW480) viability and decreased migration and invasiveness. In addition, CCAT1-shRNA significantly reduced vascular endothelial growth factor (VEGF) expression in SW480 cells; however, these effects were partially rescued by an miR-218 inhibitor. Furthermore, it was revealed that the CCAT1-plasmid significantly promoted the viability of SW480 cells, increased cell migration and invasiveness, and significantly increased VEGF expression. However, these effects were also partially rescued by with a miR-218 mimic. Taken together, the present results identified that the CCAT1/miR-218 axis serves a key role in the regulation of colorectal cancer progression, which may be used as potential therapeutic target for the treatment of colorectal cancer.

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“…CCAT2 is a 1752 bp lncRNA located on chromosomal region 8q24.21 [288,289,290]. CCAT2 is originally considered as an oncogenic lncRNA in CRC, which is significantly upregulated in CRC tissues and cell lines [279,291,292,293]. In addition, the expression level of CCAT2 in microsatellite-stable (MSS) CRC tissue samples is 10-folds higher than that in microsatellite-unstable (MSI-H) tumors and adjacent normal colon mucosa [291].…”

Section: Lncrnas In Colorectal Cancer (Crc)mentioning

confidence: 99%

“…CCAT2 has also been demonstrated to be associated with other cancers, including GC, BC, NSCLC, and ESCC [288]. A study clarified that a high level of CCAT2 expression in CRC is associated with poor cell differentiation, deep tumor infiltration, increased lymph node metastasis, advanced TNM stage, and short DFS and OS [279,292]. The serum level of CCAT2 expression in CRC patients is suggested to be significantly increased compared with that in controls [294].…”

Section: Lncrnas In Colorectal Cancer (Crc)mentioning

confidence: 99%

Long Non-Coding RNA in the Pathogenesis of Cancers

Chi

Wang

et al. 2019

Cells

643

452

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The incidence and mortality rate of cancer has been quickly increasing in the past decades. At present, cancer has become the leading cause of death worldwide. Most of the cancers cannot be effectively diagnosed at the early stage. Although there are multiple therapeutic treatments, including surgery, radiotherapy, chemotherapy, and targeted drugs, their effectiveness is still limited. The overall survival rate of malignant cancers is still low. It is necessary to further study the mechanisms for malignant cancers, and explore new biomarkers and targets that are more sensitive and effective for early diagnosis, treatment, and prognosis of cancers than traditional biomarkers and methods. Long non-coding RNAs (lncRNAs) are a class of RNA transcripts with a length greater than 200 nucleotides. Generally, lncRNAs are not capable of encoding proteins or peptides. LncRNAs exert diverse biological functions by regulating gene expressions and functions at transcriptional, translational, and post-translational levels. In the past decade, it has been demonstrated that the dysregulated lncRNA profile is widely involved in the pathogenesis of many diseases, including cancer, metabolic disorders, and cardiovascular diseases. In particular, lncRNAs have been revealed to play an important role in tumor growth and metastasis. Many lncRNAs have been shown to be potential biomarkers and targets for the diagnosis and treatment of cancers. This review aims to briefly discuss the latest findings regarding the roles and mechanisms of some important lncRNAs in the pathogenesis of certain malignant cancers, including lung, breast, liver, and colorectal cancers, as well as hematological malignancies and neuroblastoma.

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Overexpression of long non‑coding RNA colon cancer‑associated transcript 2 is associated with advanced tumor progression and poor prognosis in patients with colorectal cancer

Cited by 20 publications

References 27 publications

MicroRNA-937 is overexpressed and predicts poor prognosis in patients with colon cancer

MicroRNA-937 is overexpressed and predicts poor prognosis in patients with colon cancer

Long non‑coding RNA CCAT1 promotes colorectal cancer cell migration, invasiveness and viability by upregulating VEGF via negative modulation of microRNA‑218

Long Non-Coding RNA in the Pathogenesis of Cancers

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