Overexpression of SmSCR1 Promotes Tanshinone Accumulation and Hairy Root Growth in Salvia miltiorrhiza

Zhou, Wei; Wang, Shuai; Shen, Yilan; Liu, Yunhui; Maoz, Itay; Gao, Xiankui; Chen, Chengan; Liu, Tingyao; Wang, Can; Kai, Guoyin

doi:10.3389/fpls.2022.860033

Cited by 3 publications

(2 citation statements)

References 45 publications

(72 reference statements)

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“…In addition, there are several approaches for increasing the yield of specialized metabolites, including metabolic engineering of tanshinones [ 154 , 155 , 156 ], phenolic acids [ 155 , 157 ], flavonoids [ 158 ], and diterpenoids [ 159 ] or varying the cultivation conditions [ 155 , 157 ].…”

Section: Perspectives Of Large-scale Synthesis Of Anti-sars-cov-2 Com...mentioning

confidence: 99%

The Main Protease of SARS-CoV-2 as a Target for Phytochemicals against Coronavirus

Issa

Сокорнова

Zhidkin

et al. 2022

Plants

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In late December 2019, the first cases of COVID-19 emerged as an outbreak in Wuhan, China that later spread vastly around the world, evolving into a pandemic and one of the worst global health crises in modern history. The causative agent was identified as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Although several vaccines were authorized for emergency use, constantly emerging new viral mutants and limited treatment options for COVID-19 drastically highlighted the need for developing an efficient treatment for this disease. One of the most important viral components to target for this purpose is the main protease of the coronavirus (Mpro). This enzyme is an excellent target for a potential drug, as it is essential for viral replication and has no closely related homologues in humans, making its inhibitors unlikely to be toxic. Our review describes a variety of approaches that could be applied in search of potential inhibitors among plant-derived compounds, including virtual in silico screening (a data-driven approach), which could be structure-based or fragment-guided, the classical approach of high-throughput screening, and antiviral activity cell-based assays. We will focus on several classes of compounds reported to be potential inhibitors of Mpro, including phenols and polyphenols, alkaloids, and terpenoids.

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Section: Perspectives Of Large-scale Synthesis Of Anti-sars-cov-2 Com...mentioning

confidence: 99%

The Main Protease of SARS-CoV-2 as a Target for Phytochemicals against Coronavirus

Issa

Сокорнова

Zhidkin

et al. 2022

Plants

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show abstract

“…DELLA protein increases anthocyanin synthesis by inhibiting PAP1 protein [ 19 ]. In medicinal plants, the overexpression of SmSCR1 in Salvia miltiorrhiza resulted in significantly higher accumulation of tanshinone than that in the control group [ 20 ]. In Dendrobium officinale , eight GRAS genes were upregulated in different tissues after heat and salt stress [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Functional Study of PgGRAS68-01 Gene Involved in the Regulation of Ginsenoside Biosynthesis in Panax ginseng

Liu

Wang

Yun

et al. 2023

IJMS

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Ginseng (Panax ginseng C. A. Meyer) is a perennial herb from the genus Panax in the family Araliaceae. It is famous in China and abroad. The biosynthesis of ginsenosides is controlled by structural genes and regulated by transcription factors. GRAS transcription factors are widely found in plants. They can be used as tools to modify plant metabolic pathways by interacting with promoters or regulatory elements of target genes to regulate the expression of target genes, thereby activating the synergistic interaction of multiple genes in metabolic pathways and effectively improving the accumulation of secondary metabolites. However, there are no reports on the involvement of the GRAS gene family in ginsenoside biosynthesis. In this study, the GRAS gene family was located on chromosome 24 pairs in ginseng. Tandem replication and fragment replication also played a key role in the expansion of the GRAS gene family. The PgGRAS68-01 gene closely related to ginsenoside biosynthesis was screened out, and the sequence and expression pattern of the gene were analyzed. The results showed that the expression of PgGRAS68-01 gene was spatio-temporal specific. The full-length sequence of PgGRAS68-01 gene was cloned, and the overexpression vector pBI121-PgGRAS68-01 was constructed. The ginseng seedlings were transformed by Agrobacterium rhifaciens-mediated method. The saponin content in the single root of positive hair root was detected, and the inhibitory role of PgGRAS68-01 in ginsenoside synthesis is reported.

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Progress and prospect: Biosynthesis of plant natural products based on plant chassis

Lin,

Yin,

Zeng

et al. 2023

Biotechnology Advances

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Overexpression of SmSCR1 Promotes Tanshinone Accumulation and Hairy Root Growth in Salvia miltiorrhiza

Cited by 3 publications

References 45 publications

The Main Protease of SARS-CoV-2 as a Target for Phytochemicals against Coronavirus

The Main Protease of SARS-CoV-2 as a Target for Phytochemicals against Coronavirus

Functional Study of PgGRAS68-01 Gene Involved in the Regulation of Ginsenoside Biosynthesis in Panax ginseng

Progress and prospect: Biosynthesis of plant natural products based on plant chassis

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