Overexpression of the genes coding ascorbate peroxidase from Brassica campestris enhances heat tolerance in transgenic Arabidopsis thaliana

Chiang, Chui-Liang; Chien, Hao-Lun; Chen, L. F. O.; Hsiung, Tung-Chuan; Chiang, Ming‐Chang; Chen, S. P.; Lin, Kuan‐Hung

doi:10.1007/s10535-015-0489-y

Cited by 28 publications

(18 citation statements)

References 42 publications

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“…Overexpression of APX and Cu/Zn SOD increased the drought resistance and recovery capacity from drought stress in Ipomoea batatas (Lu et al, 2015 ). Overexpressed Brassica campestris APX gene in transgenic Arabidopsis enhanced the heat tolerance via elimination of H 2 O 2 (Chiang et al, 2015b ). Therefore, increased APX activity in cells is an indicator of the presence of stress factors.…”

Section: Resultsmentioning

confidence: 99%

Identification and Comparative Analysis of H2O2-Scavenging Enzymes (Ascorbate Peroxidase and Glutathione Peroxidase) in Selected Plants Employing Bioinformatics Approaches

et al. 2016

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Among major reactive oxygen species (ROS), hydrogen peroxide (H2O2) exhibits dual roles in plant metabolism. Low levels of H2O2 modulate many biological/physiological processes in plants; whereas, its high level can cause damage to cell structures, having severe consequences. Thus, steady-state level of cellular H2O2 must be tightly regulated. Glutathione peroxidases (GPX) and ascorbate peroxidase (APX) are two major ROS-scavenging enzymes which catalyze the reduction of H2O2 in order to prevent potential H2O2-derived cellular damage. Employing bioinformatics approaches, this study presents a comparative evaluation of both GPX and APX in 18 different plant species, and provides valuable insights into the nature and complex regulation of these enzymes. Herein, (a) potential GPX and APX genes/proteins from 18 different plant species were identified, (b) their exon/intron organization were analyzed, (c) detailed information about their physicochemical properties were provided, (d) conserved motif signatures of GPX and APX were identified, (e) their phylogenetic trees and 3D models were constructed, (f) protein-protein interaction networks were generated, and finally (g) GPX and APX gene expression profiles were analyzed. Study outcomes enlightened GPX and APX as major H2O2-scavenging enzymes at their structural and functional levels, which could be used in future studies in the current direction.

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Section: Resultsmentioning

confidence: 99%

Identification and Comparative Analysis of H2O2-Scavenging Enzymes (Ascorbate Peroxidase and Glutathione Peroxidase) in Selected Plants Employing Bioinformatics Approaches

et al. 2016

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“…It has been suggested that heat stress-tolerant cultivars contain antioxidant enzymes with higher activities, the group of such enzymes including GST, APX and CAT (Asthir 2015). In addition, transgenic Arabidopsis overexpressing the broccoli CAT gene and the Chinese cabbage APX gene exhibits enhanced heat tolerance (Chiang et al 2014(Chiang et al , 2015. The antioxidant defence system is correlated with heat tolerance and other stress-resistance mechanisms of plants (Nguyen et al 2018).…”

Section: Improving Plant Tolerance To the High Temperatures Associatementioning

confidence: 99%

CRISPR/Cas-based genome editing to improve abiotic stress tolerance in plants

Hyun

2020

Bot Serb

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Climate change is affecting agriculture in a number of ways, such as changing water distribution, daily temperatures and salinity patterns. In this regard, plant breeding innovations and genetic engineering approaches to improve abiotic stress tolerance are necessary to avoid a decline in crop yields caused by climate change during the 21st century. In the last few years, genome editing using the CRISPR/Cas system has attracted attention as a powerful tool that can generate hereditary mutations. So far, only a few studies using the CRISPR/Cas system have been reported to improve abiotic stress tolerance, but they have clearly suggested its effective role for future applications in molecular breeding to improve abiotic stress tolerance. Accordingly, the CRISPR/Cas system application is introduced in this mini-review as a way to improve abiotic stress tolerance. Although editing efficiency and target discovery for plant CRISPR/Cas systems require further improvement, CRISPR/Cas systems will be the key approach to maintaining global food security during climate change.

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“…An OsAPX gene overexpressed in transgenic rice could enhance tolerance to chilling at the booting stage (Sato et al 2011). Overexpression of peanut APX2 and 3 genes in Arabidopsis has improved seed germination rate, and transgenic displayed tremendous heat tolerance compared to WT, through efficient elimination of cellular H 2 O 2 (Chiang et al 2015). Transgenic rice overexpressing a cytosolic GR of Brassica campestris showed tolerance towards intensive photo-oxidative stress (Kouril et al 2003).…”

Section: Enzymaticmentioning

confidence: 99%

Biotechnological Perspective of Reactive Oxygen Species (ROS)-Mediated Stress Tolerance in Plants

Chakradhar

Mahanty

Reddy

et al. 2017

Reactive Oxygen Species and Antioxidant Systems in Plants: Role and Regulation Under Abiotic Stress

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All environmental cues lead to develop secondary stress conditions like osmotic and oxidative stress conditions that reduces average crop yields by more than 50% every year. The univalent reduction of molecular oxygen (O 2) in metabolic reactions consequently produces superoxide anions (O 2 •À) and other reactive oxygen species (ROS) ubiquitously in all compartments of the cell that disturbs redox potential and causes threat to cellular organelles. The production of ROS further increases under stress conditions and especially in combination with high light intensity. Plants have evolved different strategies to minimize the accumulation of excess ROS like avoidance mechanisms such as physiological adaptation, efficient photosystems such as C4 or CAM metabolism and scavenging mechanisms through production of antioxidants and antioxidative enzymes. Ascorbate-glutathione pathway plays an important role in detoxifying excess ROS in plant cells, which includes superoxide dismutase (SOD) and ascorbate peroxidase (APX) in detoxifying O 2 •À radical and hydrogen peroxide (H 2 O 2

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Overexpression of the genes coding ascorbate peroxidase from Brassica campestris enhances heat tolerance in transgenic Arabidopsis thaliana

Cited by 28 publications

References 42 publications

Identification and Comparative Analysis of H2O2-Scavenging Enzymes (Ascorbate Peroxidase and Glutathione Peroxidase) in Selected Plants Employing Bioinformatics Approaches

Identification and Comparative Analysis of H2O2-Scavenging Enzymes (Ascorbate Peroxidase and Glutathione Peroxidase) in Selected Plants Employing Bioinformatics Approaches

CRISPR/Cas-based genome editing to improve abiotic stress tolerance in plants

Biotechnological Perspective of Reactive Oxygen Species (ROS)-Mediated Stress Tolerance in Plants

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