Overexpression of the StP5CS gene promotes nodulation and nitrogen fixation in vegetable soybean under drought stress

Abstract

The StP5CS (GenBank accession number: JN606861) T 6 homozygous transgenic lines (HTLs) of vegetable soybean [Glycine max (L.) Merrill] were grown using vermiculite pot culture to determine whether StP5CS overexpression would enhance nodulation and symbiotic nitrogen fixation (SNF) in two T 6 HTLs (17W-1, 17W-2) under drought conditions. The growth performance, nodule development and seed weight of T 6 HTLs were significantly better than those of wild type (WT) plants. The proline levels in various tissues of T 6 HTLs were higher than WT plants. The concentrations of total ureide, total N, leghemoglobin (Lb) and the activity of glutamine synthetase (GS, EC 6.3.1.2) in the T 6 HTLs were significantly increased. Moreover, the relative expression levels of five key nodulation-and SNF-associated genes (i.e., GmLba, GmGS1β1 and GmGS1β2) were significantly higher in T 6 HTLs. In conclusion, overexpression of StP5CS enhances nodulation and SNF in transgenic vegetable soybean under drought stress conditions.

Promoter of Vegetable Soybean GmTIP1;6 Responds to Diverse Abiotic Stresses and Hormone Signals in Transgenic Arabidopsis

“…Drought, salt, and cold stresses severely affected the yield and quality of vegetable soybeans [ 6 , 7 , 8 ]. Currently, researches on the abiotic stress response mechanism of vegetable soybean are extremely inadequate [ 9 , 10 , 11 , 12 ]. It is promising to discover genes responsive to environmental factors and develop cultivars tolerant to abiotic stresses for vegetable soybean, especially given the impacts of global climate changes.…”